In vitro induction of anti-DNP antibody by immunogenic RNA from rabbit liver

Duke, Linda J.; Harshman, Sidney

doi:10.1016/0019-2791(71)90506-4

Cited by 12 publications

(1 citation statement)

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“…Assays were made essentially by the method ot' Yamada and Yamada (28) as modified by Duke and Harshman (5). In brief', spleen cells and SRBC were mixed with 0.7% agarose, and the mixture was allowed to drop onto a petri plate surface from a 5-ml pipette that was calibrated to deliver 16 drops/ml, each drop containing 106 spleen cells.…”

Section: Methodsmentioning

confidence: 99%

Preliminary Characterization of “Immunogenic” Ribonucleic Acid Derived from Rat Peritoneal Exudate Cells

Archer

Wust

1973

Infect Immun

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An "immunogenic" ribonucleic acid (Im-RNA) has been extracted from peritoneal exudate (PE) cells of rats that were immunized with sheep erythrocytes (SRBC). Following multiple phenol extractions and deoxyribonuclease treatment, the material obtained from PE cells was eluted from diethylaminoethyl-cellulose at 0.55 M NaCl concentration and partially purified in this procedure by a factor of 7to 10-fold. After column chromatography, Im-RNA was found to be free of antigen based on results using 51Cr-labeled SRBC or '4C-dinitrophenol coupled to methylated bovine serum albumin as antigens. The Im-RNA showed a biphasic hyperchromicity curve when heated. The first phase, from 30 C to 90 C was gradual, accounting for 15.2% hyperchromicity suggestive of transfer RNA melting. No loss in immunogenic activity was observed when the Im-RNA was heated to 90 C. The second phase, from 90 C to 102 C, accounting for 15.2% further hyperchromicity, had a calculated Tm of 96 C. Heating above 90 C resulted in an irreversible loss of immunogenic activity. These results strongly suggested that the RNA fraction contained a highly ordered secondary structure such as might be found with double-stranded nucleic acid. The nature and function of the Im-RNA is discussed.

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Section: Methodsmentioning

confidence: 99%