In vitro induction of cytotoxicity and DNA strand breaks in CHO cells exposed to cypermethrin, pendimethalin and dichlorvos

Assessment of Genotoxic Effects of Pendimethalin in Chinese Hamster Over Cells by the Single Cell Gel Electrophoresis (Comet) Assay

“…Patel et al 8 were evaluated the cytotoxic effects of PM in CHO cells after application of PM for 3 h at 1, 10 , 100, 1000 and 10000 mM concentrations by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method, then they determined the DNA damage by comet assay. The results of this study show that, the IC 50 value of PM is close to the 10000 mM.…”

“…The 90% cell viability after 3 h of exposure of PM is determined in 10-100 mM. Patel et al 8 also report over 90% of cell survival by trypan blue staining method in CHO cells which are exposed to 0.01, 0.1, 1, 10 and 100 mM concentrations of PM for 3 h. The DNA damage is investigated by comet assay in terms of the arbitrary units, % tail DNA and tail length. PM is found to cause a significant concentration dependent increase in DNA damage in the studied variables, at the concentrations of 0.1 to 100 mM (p<0.05).…”

“…PM achieved, concentration dependent (0.1-100 mM) induction in DNA damage evaluated by Comet assay in CHO cells. 8 PM decreased the root bundle length and increased the mitotic index and the percentage of chromosome aberrations dose dependently in maize and onion. 10 It has been reported to be a contaminant for the environment and found highly toxic for fish and aquatic invertebrates.…”

“…6 It also shows cytotoxic effect in FRTL 5 (rat thyroid) 7 and Chinese hamster over (CHO) cell line. 8 Dimitrov et al 9 (2006) has shown that PM significantly increased the chromosomal aberrations at 489 mg/kg dose in mouse bone marrow. In addition, it induced the micronucleus frequency in plant cell and mouse bone marrow polychromatic erythrocytes.…”

Metabolism of Pyrethroids by Mosquito Cytochrome P450 Enzymes: Impact on Vector Control

“…This can be accomplished because other than targeting on sodium channels of nervous system, pyrethroids possess toxic effects on cells such as inhibition of cell mitochondrial complex I or causing DNA damage and cell death (Gassner et al, 1997;Patel et al, 2007;Naravaneni & Jamil, 2005). Similar cell death and cytotoxic to cells caused by organophosphates and carbamate insecticides have also been reported (Maran et al, 2010;Schmuck & Mihail, 2004).…”