In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.)

Agrawal, Dinesh Chandra; Banerjee, Anjan K.; Kolala, R. R.; Dhage, A. B.; Kulkarni, Anuradha; Nalawade, S. M.; Hazra, Sulekha; Krishnamurthy, K. V.

doi:10.1007/bf01275508

Cited by 43 publications

(9 citation statements)

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“…Similar to the results in the present study, the differential effect of various concentrations of BAP on the induction of multiple shoots has earlier been reported for Gossypium (Agrawal et al [1997]), Salix (Agrawal and Gebhardt [1994]), Pisum (Jackson and Hobbs [1990]) Phaseolus (McClean and Grafton [1989]) and Glycine (Cheng et al [1980]). Akin to the present study, BAP was the most effective cytokinin in all these reports, indicating a particular cytokinin preference for multiple shoot induction in certain tissues.…”

Section: Resultssupporting

confidence: 91%

“…Higher concentrations of both the auxins (>3.0 mg/L IAA and > 0.1 mg/L IBA) induced more average number of roots per shoot, however, shoots induced callus at the base which affected the survival rates of the rooted shoots in the greenhouse (Table 3). The promotory effect of a lower salt concentration of MS on in vitro rooting of shoots has been reported for Gossypium (Agrawal et al [1997]), Philodendron spp. (Maene and Debergh [1985]).…”

Section: Resultsmentioning

confidence: 98%

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In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

et al. 2014

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BackgroundGlossogyne tenuifolia Cassini (Hsiang-Ju in Chinese) is a perennial herb native to Penghu Islands, Taiwan. The herb is a traditional anti-pyretic and hepatoprotective used in Chinese medicine. Several studies on G. tenuifolia have demonstrated its pharmacological values of antioxidation, anti-inflammation, immunomodulation, and cytotoxicity on several human cancer cell lines. Active compounds, oleanolic acid and luteolin in G. tenuifolia are affected by several factors, including climatic change, pathogens and agricultural practices. Plant population of G. tenuifolia has been severely affected and reduced considerably in natural habitat due to the use of herbicides by farmers. Also, collection of plant material from the natural habitat is restricted to a few months in a year. Therefore, the objective of the present study was to develop an efficient micropropagation protocol for G. tenuifolia. The study also aimed to investigate the influence of in vitro growth environment on the active compounds in in vitro shoots, tissue culture raised greenhouse plants; compare the values with wild plants and commercially available crude drug.ResultsHalf-strength MS (Murashige and Skoog) basal medium supplemented with 0.1 mg/L 6-benzyladenine (BA) and 0.1 mg/L α-naphthaleneacetic acid (NAA) induced the maximum average number of shoots (7.3) per shoot tip explant excised from in vitro grown seedlings. Induction of rooting in cent percent in vitro shoots with an average number of 6.6 roots/shoot was achieved on ½ strength MS medium supplemented with 3.0 mg/L indole-3-acetic acid (IAA). The rooted plantlets acclimatized successfully in the greenhouse with a 100% survival rate. HPLC analysis revealed that the quantity of oleanolic acid and luteolin in in vitro shoots, tissue culture plants in the greenhouse, wild type plants and commercial crude drug varied depending upon the source. The oleanolic acid and luteolin contents were found to be significantly higher (16.89 mg/g and 0.84 mg/g, respectively) in 3-month old tissue culture raised plants in greenhouse compared to commercially available crude drug (6.51 mg/g, 0.13 mg/g, respectively).ConclusionsWe have successfully developed an in vitro propagation protocol for G. tenuifolia which can expedite its plant production throughout the year. The contents of oleanolic acid and luteolin in the tissue culture raised plants in the greenhouse were significantly higher than the marketed crude drug demonstrating the practical application of the tissue culture technology. These findings may be very useful in micropropagation, germplasm conservation and commercial cultivation of G. tenuifolia. So far, there is no published report on tissue culture propagation of this important medicinal plant species.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-014-0045-7) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 98%

In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

et al. 2014

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“…Similar to the present study, BAP was found to be the most effective cytokinin in several plant species, i.e. Gossypium (Agrawal et al [1997]), Salix (Agrawal and Gebhardt [1994]), Pisum (Jackson and Hobbs [1990]), Phaseolus (McClean and Grafton [1989]) and Glycine (Cheng et al [1980]), indicating a particular cytokinin preference of certain tissues for the induction of multiple shoots. In an earlier report on G. scabra , induction of multiple shoots in axillary buds of was achieved on MS medium supplemented with gibberlliin A 3 (GA 3 ) and BA (1 mg/L −1 each) (Yamada et al [1991]).…”

Section: Resultssupporting

confidence: 84%

“…Different plant species respond differently to auxins for the induction of rooting. The promotory effect of a lower salt concentration of MS on in vitro rooting of shoots has been reported for Gossypium (Agrawal et al [1997]), Philodendron spp. (Maene and Debergh [1985]).…”

Section: Resultsmentioning

confidence: 98%

In vitro propagation of Gentiana scabra Bunge – an important medicinal plant in the Chinese system of medicines

Huang

Agrawal

et al. 2014

Bot Stud

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BackgroundGentiana scabra Bunge commonly known as ‘Long dan cao’ in China has been used in traditional Chinese medicines for more than 2000 years. Dry roots and rhizome of the herb have been used for the treatment of inflammation, anorexia, indigestion and gastric infections. Iridoids and secoiridoids are the main bioactive compounds which attribute to the pharmacological properties of this plant. The species is difficult to mass propagate by seed due to the low percentage of germination and limited dormancy period. Wild populations in some locations are considered to be in the endangered category due to over exploitation.ResultsIn the present study, we report an efficient micropropagation system. Shoot apices of six weeks old in vitro grown G. scabra plants were used as explants for the in vitro propagation. Induction of multiple shoots (9.1/explant) was achieved on the culture of shoot apices on half strength Murashige and Skoog’s basal medium (MSBM) containing 2.0 mg/L−1 6-benzylaminopurine (BA), 3% sucrose and 0.9% Difco agar. In vitro shoots induced profuse rooting on half strength of MSBM supplemented with 0.1 mg/L−1 1-naphthaleneacetic acid (NAA), 3% sucrose and 0.3% gelrite. A two-stage ventilation closure procedure during the in vitro culture, and transparent sachet technique enhanced the survival rate of G. scabra plantlets to 96% in the greenhouse. Tissue culture plants flowered after 5 months of transfer to pots.ConclusionsA simple and an efficient in vitro propagation protocol of Gentiana scabra Bunge by optimizing the medium composition and ventilation closure treatments has been developed. The protocol can be very useful in germplasm conservation and commercial cultivation of G. scabra plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-014-0056-4) contains supplementary material, which is available to authorized users.

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“…Requirement of contamination free explant is essential for plant tissue culture, because throughout the study, the surface sterilized seeds and seedlings derived plant parts were used as explants [4]. Seed dormancy is a temporary failure or block of a viable seed to complete germination [5].…”

Section: Discussionmentioning

confidence: 99%

An <i>in Vitro</i> Approach for the Conservation of <i>Meizotropis pellita</i>: An Endangered and Endemic Plant

Singh¹,

Nailwal²,

Tewari³

2013

AJPS

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An efficient protocol for high frequency in vitro regeneration of Meizotropis pellita an endangered and endemic plant was developed. In vitro germination of the seeds of Patwa was achieved successfully after incubation for 10 -15 days and plantlets up to a height of 5 -7 cm with profuse rooting were observed after incubation for 7 weeks in hormone free MS medium. This in vitro germination totally reduced contamination that used to occur in the explant taken from the field. Callus induction and proliferation were observed in leaf explants after 15 -20 days of incubation in MS medium containing 2 -4, D (9.06 μM) alone or in combination with 2 -4, D (9.06 μM) + 2-iP (7.38 μM). Shoot regeneration was achieved from 2 months old callus explant in MS medium supplemented with BA (17.6 μM) + GA3 (1.0 μM). Shoot multiplication was also achieved from cotyledonary node of M. pellita in MS medium supplemented with Kinetin + GA3 (4.6 μM + 1.0 μM) or BA (13.2, 17.6 μM) + GA3 (1.0 μM) after 30 -45 days of incubation. IBA (4.9 μM) was more effective in root regeneration from micro shoots. The plantlets after acclimatization over a period of 1 month were further hardened in a polyhouse for two months. On the basis of available literature this is the first and significant study regarding the comparative effect of different PGRs on in-vitro propagation of Meizotropis pellita by using different explants and their subsequent effect on rooting. This significant study could be useful for large scale production of successfully hardened plants and conservation of this shrub.

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In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.)

Cited by 43 publications

References 25 publications

In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

In vitro propagation of Gentiana scabra Bunge – an important medicinal plant in the Chinese system of medicines

An <i>in Vitro</i> Approach for the Conservation of <i>Meizotropis pellita</i>: An Endangered and Endemic Plant

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In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.)

Cited by 43 publications

References 25 publications

In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

In vitro propagation of Gentiana scabra Bunge – an important medicinal plant in the Chinese system of medicines

An &lt;i&gt;in Vitro&lt;/i&gt; Approach for the Conservation of &lt;i&gt;Meizotropis pellita&lt;/i&gt;: An Endangered and Endemic Plant

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An <i>in Vitro</i> Approach for the Conservation of <i>Meizotropis pellita</i>: An Endangered and Endemic Plant