In vitro induction of nitric oxide by mouse peritoneal macrophages treated with human placental extract

Chakraborty, Piyali Datta; Bhattacharyya, Debasish; Pal, Swati; Ali, Nahid

doi:10.1016/j.intimp.2005.07.018

Cited by 37 publications

(25 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The extract also exhibits anti-microbial activity against commonly occurring pathological organisms [17], in vitro induction of NO by mouse peritoneal macrophages [18] along with enhancement of cell adhesion [19]. These findings emphasize the regulatory action exhibited by the placental extract.…”

Section: Introductionmentioning

confidence: 88%

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Chakraborty¹,

Goswami²,

Bera³

et al. 2014

AJAC

Self Cite

View full text Add to dashboard Cite

Characterization of an aqueous extract of human placenta, used as a licensed drug for wound healing, leads to the identification of several bioactive components including polydeoxyribonucleotides (PDRNs). PDRNs are mixture of DNA fragments of different molecular weight. A spectrofluorimetric method of quantitation of PDRNs in the aqueous extract of human placenta by using ethidium bromide (EtBr) has been described here. It has been demonstrated by thin layer chromatography (TLC) followed by reversed phase HPLC that EtBr binds specifically with the PDRN fraction of the multi-component extract. The binding specificity of EtBr has been verified by the analysis of emission spectra of the extract. A concentration of 0.29 µg/ml EtBr exhibits a linear range of standard CT-DNA from 0.5 -5 µg/ml of buffer (R 2 = 0.992). The same concentration of EtBr shows a linear range of measurements of placenta extract from 5 -35 µl/ml of buffer (R 2 = 0.976). The points of the curve were the average of three sets where maximum variation observed was ±3%. PDRN content of the extract has been estimated based on the resultant fluorescence emission (after background correction) with respect to the standard calibration curve of calf thymus DNA (CT-DNA). Estimation of PDRN in a large number of batches of placenta extract (n = 100) has been done. The statistical analysis of the estimation was found to be significant and the lower and upper levels of PDRN were 158.30 and 239.03 µg/ml of the extract respectively. This easy-to-use method of estimation of PDRN in multi-component biological extract is reported for the first time. This will help in quantitation of PDRNs for other biological extracts.

show abstract

Section: Introductionmentioning

confidence: 88%

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Chakraborty¹,

Goswami²,

Bera³

et al. 2014

AJAC

Self Cite

View full text Add to dashboard Cite

show abstract

“…It promotes inflammatory mediation of repair mechanism and wound matrix development followed by remodeling. The extract has also shown to induce interferon- (IFN-) production by macrophages (Chakraborty et al, 2006). Anti-microbial property of the extract against a large number of pathological microorganisms related to prevention of secondary infections to wounds has also been demonstrated.…”

Section: Biochemical Characterization and Possible Mechanism Of Actiomentioning

confidence: 98%

“…The aqueous extract has been investigated in terms of induction of NO by macrophages. It has been demonstrated that with an increase of NO production, concomitant decrease in NADPH present in the applied placental extract was observed, thereby indicating that the NADPH pool of placental extract is metabolized, further justifying the biological potency of this nucleotide in the extract (Chakraborty et al, 2006). It has been well documented that nitric oxide (NO) has multiple effects at the molecular, cellular and physiological level in wound healing.…”

Section: Biochemical Characterization and Possible Mechanism Of Actiomentioning

confidence: 99%

Aqueous Extract of Human Placenta

Chakraborty¹,

Bhattacharyya²

2012

Recent Advances in Research on the Human Placenta

View full text Add to dashboard Cite

“…К настоящему времени было предложе-но несколько различных молекулярных и физиологиче-ских механизмов, объясняющих ранозаживляющий эффект ГПЧ: увеличение уровней ростовых факторов фибробластов (ФРФ) [12], быстрая миграция нейтрофи-лов к ране [13], синтез NO, важнейшего клеточного меди-атора в заживлении ран [14]. В частности, в составе неко-торых препаратов на основе ГПЧ был найден противо-воспалительный дипептид JBP485 (цикло-транс-4-гидроксипролилсерин), который снижает уровни секре-ции и ФНО-альфа и проявляет антиапоптотический эффект (снижение уровней и активности каспазы-3 -фермента-активатора апоптоза клеток [15]).…”

Section: молекулярнофизиологические механизмы действия гпч на регенеunclassified

Human Placenta Hydrolyzates in Reconstructive Therapy of Connective Tissue of Cartilage and Joints

Громова¹,

Торшин²,

Диброва³

et al. 2017

Med. sov.

View full text Add to dashboard Cite

In vitro induction of nitric oxide by mouse peritoneal macrophages treated with human placental extract

Cited by 37 publications

References 37 publications

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Aqueous Extract of Human Placenta

Human Placenta Hydrolyzates in Reconstructive Therapy of Connective Tissue of Cartilage and Joints

Contact Info

Product

Resources

About