In vitro induction of polyploidy in japanese quince (Chaenomeles japonica)

Stanys, V.; Weckman, A.; Stanienė, G.; Duchovskis, Pavelas

doi:10.1007/s11240-005-9029-3

Cited by 77 publications

(75 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Compared to diploids, the newly obtained allotetraploids had larger stomata, pollen grains, leaves, and flowers. Similar observations concerning enhancement of cells, stomata, and organs were noted in newly obtained autotetraploids of Zantedeschia (Cohen and Yao 1996), Chaenomeles japonica (Stanys et al 2006), potato (Stupar et al 2007), Nicotiana sp. (Anssour et al 2009), and Gerbera jamesonii (Gantait et al 2011).…”

Section: Discussionsupporting

confidence: 85%

Phenotypic and genome size changes (variation) in synthetic tetraploids of daylily (Hemerocallis) in relation to their diploid counterparts

et al. 2014

View full text Add to dashboard Cite

Tetraploids of daylily have taken a leading position among the daylily cultivars due to desirable traits such as vigorous growth and flowers with more intense colour. In our previous studies, several tetraploids of daylily cultivars 'Blink of an Eye' and 'Berlin Multi' were obtained using in vitro techniques with different antimitotic agents (colchicine, oryzalin, trifluralin, and amiprophos methyl). The purposes of this study were to evaluate changes in daylily tetraploids in relation to their diploid counterparts and to assess variation among diploids and tetraploids derived from genetically homogenous plant material treated with antimitotic agents. In the first year of the ex vitro cultivation, growth of tetraploids was poorer in comparison with diploids, but in the second year, tetraploid growth was much more vigorous. Compared to diploids, in tetraploids of both cultivars percentage of flowering plants was lower, flowering was delayed by 8 days and 1 month in 'Blink of an Eye' and 'Berlin Multi', respectively, and bud number per scape was lower by approximately 20 and 40 %, respectively. Tetraploid leaves and flowers of both cultivars were significantly larger, chlorophyll concentration index was higher by approximately 40 %, and stomata were longer by 35 %. In 'Berlin Multi' tetraploids, inflorescence stems were shorter by 20 %. In 'Blink of an Eye', variation in flower colour tone and shape and stamen malformation rate was detected both within the unconverted diploids and tetraploids but was more evident in tetraploids. In both cultivars, variation was also found in the nuclear DNA content, which ranged in diploids and tetraploids, respectively, 8.02-8.53 and 16.01-17.13 pg in 'Berlin Multi ' and 8.28-8.71 and 15.93-17.36 pg in 'Blink of an Eye'. Since the variation, while less evident, also occurred in the diploids (regenerated from the antimitotic treated material), we suppose that these variations could be due to antimitotic agents that can induce not only chromosome doubling but also chromosomal and gene mutations. The extent and character of these changes can be related to parental genotype and/or antimitotic agent. Further research is required at the cytological and molecular level to explain the character of changes, epigenetic and/or genetic.

show abstract

Section: Discussionsupporting

confidence: 85%

Phenotypic and genome size changes (variation) in synthetic tetraploids of daylily (Hemerocallis) in relation to their diploid counterparts

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The success of polyploidy induction depends on several variables: plant species, antimitotic agents, explant types, exposure times and concentrations (Dhooghe et al, 2011). Traditionally, colchicine treatment is performed on germinating seeds (Wu et al, 2010) and 7-day old seedlings (Abd El-Naby et al, 2012), apical meristems (Schifino, Morales Fernandes, 1987), apical buds (Wang et al, 2009;Talukdar, 2010), axilary meritems (Anderson et al, 1991), epicotyls (Bliznyuk, Filipovich, 2007) and cotyledons (Stanys et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Production of Trifolium pratense L. and T. hybridum L. tetraploid populations and assessment of their agrobiological characteristics

Dabkevičienė

Statkevičiūtė

Mikaliūnienė

et al. 2016

Zemdirbyste-Agriculture

View full text Add to dashboard Cite

The current study was set out to establish the efficacy of different mitosis inhibitors (colchicine, amiprophos-methyl, trifluralin and oryzalin) used in the production of red clover (Trifolium pratense L.) and alsike clover (Trifolium hybridum L.) polyploids and to assess the agrobiological traits of tetraploid populations. Four cultivars and five wild populations of T. pratense, one cultivar and three wild populations of T. hybridum were used for polyploidization. The effect of conventional soaking of germinating seeds in a colchicine solution was compared with that of colchicine treatment of embryos in an in vitro culture. Colchicine treatment of T. pratense embryos resulted in 3.3 times higher production of tetraploids (teraploid yield 55.0%) compared with colchicine treatment of seedlings, and the production of chimeric plants was 1.9 times lower. The different mitosis inhibitors were characterised by a diverse polyploidization effect. Tetraploid yield depended not only on the mitosis inhibitor used but also on the plant species. Treatment of T. pratense embryos with colchicine, amiprophosmethyl and oryzalin yielded similar results in terms of tetraploid production (31.3-40.7%) and chimeras (14.3-22.4%). The concentration of trifluralin used for the production of T. pratense tetraploids was ineffective. Colchicine solution was more efficient for the development of T. hybridum tetraploids whose yield was 2.5 times as high as that obtained having treated the embryos with amiprophos-methyl. Induced tetraploid populations were compared to reference cultivars in field trials. The two T. pratense populations 'Radviliai 4n' and 'Arimaičiai 4n' stood out in this respect -their plants accumulated higher dry matter contents, produced more inflorescences than those of the reference cultivar and exhibited prolific seed yield capacity. In relation to the agrobiological traits, the tetraploid populations of T. hybridum did not surpass the reference cultivar.

show abstract

“…Polyploidy induction by antimitotic agents, such as colchicine, APM, oryzalin, and trifluralin by arresting mitosis at the anaphase stage, play an increasingly important role in crop breeding (Thao et al, 2003;Stanys et al, 2006). Although considerable progress has been made in the induction and production of Allium plants by antimitotic agents (Campion et al, 1995;Grzebelus and Adamus, 2004), there is no report on tetraploid garlic induction by trifluralin.…”

Section: Discussionmentioning

confidence: 99%

“…The chromosome doubling agent should be not only efficient but also of low toxicity, and the explant should be reliable in propagation. In the production of crop polyploids, besides colchicine, other microtubule depolymerizing compounds, such as oryzalin, trifluralin and amiprophos-methyl (APM) at various concentrations and for various treatment durations have been tested (Wang and Wang, 2000;Qin et al, 2006;Stanys et al, 2006). A few efforts have also been directed at garlic polyploidy induction (Zhang and Li, 2006b;Xie and Deng, 2009).…”

Section: Introductionmentioning

confidence: 99%

In vitro induction of tetraploid garlic with trifluralin

Cheng

Zhou²,

Khan³

et al. 2012

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Garlic (Allium sativum) is propagated asexually. Since sexual cross breeding is almost impossible, means for effective breeding are not currently available and the available production cultivars are seriously aged and degenerated. A possible alternative for breeding is chemical induction. Trifluralin, a type of herbicide, has been reported to provoke chromosome doubling. However, this chemical had not been tested on garlic. We tested various trifluralin concentrations and treatment durations for efficiency in the induction of tetraploid garlic. A clove base of garlic with a stem cv. Gailiang was used as the ex-plant to induce calluses on Murashige and Skoog (MS) medium; the calluses were then inoculated onto MS medium containing different levels of trifluralin and cultured to induce chromosome number variation in vitro. Garlic calluses were effectively induced via the ex-plant and both shoots and roots differentiated well on MS medium containing 6-benzylaminopurine at 3.0 mg/L and indole-3-acetic acid at 0.1 mg/L. However, increases in trifluralin concentration and treatment duration reduced the survival rate and differentiation rate of calluses. Garlic callus cultured for 15 days on medium containing 100 μM trifluralin gave the highest rate of chromosome doubling. Through observation of chromosome number in the root apical cells and the morphology ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (3): 2620-2628(2012 Induction of tetraploid garlic with trifluralin 2621 of guard cells on the leaf epidermis of the regenerated plantlets, it was clear that chromosome number variation was induced and tetraploids were produced in vitro by trifluralin treatment.

show abstract

In vitro induction of polyploidy in japanese quince (Chaenomeles japonica)

Cited by 77 publications

References 16 publications

Phenotypic and genome size changes (variation) in synthetic tetraploids of daylily (Hemerocallis) in relation to their diploid counterparts

Phenotypic and genome size changes (variation) in synthetic tetraploids of daylily (Hemerocallis) in relation to their diploid counterparts

Production of Trifolium pratense L. and T. hybridum L. tetraploid populations and assessment of their agrobiological characteristics

In vitro induction of tetraploid garlic with trifluralin

Contact Info

Product

Resources

About