In vitro induction of tetraploid plants from a diploid Japanese pear cultivar ( Pyrus pyrifolia N. cv. Hosui)

Kadota, Masanori; Niimi, Yoshiji

doi:10.1007/s00299-002-0509-1

Cited by 64 publications

(7 citation statements)

References 15 publications

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“…Anti-mitotic colchicine has proved to be an effective agent for inducing polyploidy, At present, polyploidy has been successfully induced in vivo or in vitro by colchicine in a series of crop species, including banana (Hamill et al 1992;Van Duren et al 1996), mulberry (Chakraborti et al 1998), corn (Barnabas et al 1999), ginger (Adaniya and Shira 2001), hops (Roy et al 2001), pear (Kadota and Niimi 2002), pomegranate (Shao et al 2003), Miscanthus sinensis (Petersen et al 2003), Bixa orellana (Carvalho et al 2005), Platanus acerifolia (Liu et al 2007),Vaccinium darrowii (Chavez and Lyrene 2009), Phlox subulata L. (Zhang et al 2008), and Helleborus (Dhooghe and Van Labeke 2007;Dhooghe et al 2009a, b). Among others, induction of polyploidy has been achieved in many Araceae members, such as Xanthosoma (Esnard et al 1993), Zantedeschia , Alocasia (Thao et al 2003), Spathiphyllum wallisii Regel (Eeckhaut et al 2004), Zamioculcas Zamiifolia (Vanzie-Canton and Leonhardt 2009).…”

Section: Introductionmentioning

confidence: 99%

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

et al. 2011

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Tetraploids plants of Anthurium andraeanum ''Arizona'' were successfully induced after treating diploid tissue masses with colchicine. Masses originating from diploid aerial roots were treated with colchicine at three different concentrations (i.e., 0.1, 0.2, 0.3%) for about 3, 5 and 7 h, and then were transferred into Murashige and Skoog medium containing 3 mg/l BAP ? 0.2 mg/l 2,4-D. After 60 days, the survival rate and numbers of regenerative shoots were scored. The high concentration and longer duration sharply reduced survival rate. In contrast, the regeneration of plantlets was not noticeably affected by colchicine. Tetraploid plants were obtained in all treatments, but the percentage of induced tetraploids ranged from 0.2 to 7.6%. The best induction was obtained with a 5-h, treatment with 0.3% colchicine. The stomatal size of tetraploid plants was larger than in diploid plants; however, the stomatal density was lower than in diploid plants. Tetraploid plants possessed stronger petioles, thicker and deeper green leaves, and thicker and longer lived spathes in comparison with diploid plants. Abnormal spathes, such as double spathes or those lacking pedicels, were observed in tetraploid plants. Tetraploid plantlets could be regenerated via aerial roots; this technique could be applied to tetraploid plant propagation.

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Section: Introductionmentioning

confidence: 99%

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

et al. 2011

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“…However, works claiming the regeneration of plants showing genetic variability as a consequence of prolonged cultures are also common (von Aderkas et al 2003;von Arnold 2008). Chromosome alteration in cultures is usually harmful but, in some cases, plants with abnormal chromosome numbers can be useful for breeding purposes (Koornneef et al 1989;Kadota and Niimi 2002;Bairu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo

Currais

Loureiro

Santos

et al. 2013

Plant Cell Tiss Organ Cult

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Ploidy levels of short-term (1 and 2 years) and long-term (7 and 10 years) embryogenic cultures as well as of regenerated plantlets of tamarillo were analyzed by flow cytometry and chromosome counts. Embryogenic cultures were induced from expanding leaves cultured in the presence of Picloram or 2,4-dichlorophenoxyacetic acid (2,4-D) and monthly subcultured on the same media. Embryo development and plantlets were obtained following subculture of the embryogenic tissue in auxin free medium containing gibberellic acid (GA 3). Seedlings and rooted shoots from axillary shoot proliferation were used as controls. The results showed that in long-term embryogenic cultures the ability to develop somatic embryos and plantlets was reduced. Embryogenic tissues maintained for 10 years were mostly aneuploids of the tetraploid (2n = 4x = 48) level whereas those kept in culture for 7 years or less were also mostly aneuploids but of the diploid (2n = 2x = 24) level. The results obtained by flow cytometry were, in general, consistent with those obtained by chromosome counts. The chromosome alteration observed in the embryogenic tissues was already present after 1 year of culture and increased with culture age, hence impairing the maintenance of these tissues for long periods without affecting chromosome stability of the regenerated plantlets. However, the occurrence of triploids and tetraploids as well as aneuploids can be useful for breeding purposes. A value around 23 pg/2C was found for the genome size of tamarillo largely exceeding the value previously published (15.50 pg/2C).

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“…in Jatropha plants. As suggested by [10], the significant difference in width and length of guard cells was possibly due to the fact that most of the normal seeds were diploid (colchicine was not applied). However, when different concentrations of colchicine were applied, it would have resulted in different types of mixploidy.…”

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confidence: 93%

Improving Growth Performance of <i>Jatropha curcas</i> by Inducing Polyploidy through Colchicine Treatment

Manzoor¹,

Riaz²,

Zafar³

et al. 2016

AJPS

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This study was planned to assess genetic variation in Jatropha curcas as affected by colchichine treatments. During the experiment, seeds of Jatropha curcas were collected from mature and healthy Jatropha plants and were soaked in different concentrations of colchicines. The second treatment was that of time of soaking (seeds were soaked in different concentrations for different time durations). The results clearly revealed that by increasing the concentration of colchicine treatment, parameters like stomatal density, and size of the guard cells were reduced.

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In vitro induction of tetraploid plants from a diploid Japanese pear cultivar ( Pyrus pyrifolia N. cv. Hosui)

Cited by 64 publications

References 15 publications

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo

Improving Growth Performance of <i>Jatropha curcas</i> by Inducing Polyploidy through Colchicine Treatment

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In vitro induction of tetraploid plants from a diploid Japanese pear cultivar ( Pyrus pyrifolia N. cv. Hosui)

Cited by 64 publications

References 15 publications

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo

Improving Growth Performance of &lt;i&gt;Jatropha curcas&lt;/i&gt; by Inducing Polyploidy through Colchicine Treatment

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Improving Growth Performance of <i>Jatropha curcas</i> by Inducing Polyploidy through Colchicine Treatment