In vitro induction of tumour-specific immunity V. Detection of common antigenic determinatnts of murine fibrosarcomas

Burton, Robert C.; Warner, Noel L.

doi:10.1038/bjc.1978.24

Cited by 19 publications

(6 citation statements)

References 33 publications

(20 reference statements)

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“…The simple excision of growing tumor nodules, a procedure which elicits an effective transplantation immunity to TSTA, was insufficient to generate detectable anti-tumor PEC in our assay. Restimulation of such primed PEC by in v i m homologous tumor cells proved to be difficult due to the release of lymphocytotoxic substances by fibrosarcoma cells and to the appearance of strong cross-reacting reactions which may obscure the specific lysis against unique determinants (Burton and Warner, 1978).…”

Section: Discussionsupporting

confidence: 71%

“…In addition to the unique antigens, the cold inhibition experiment revealed a possible presence of weak common antigens which have been detected on similar tumors by both in vivo and in vitro studies and suggested to be either virus-related or fetal determinants (Burton and Warner, 1978;Hellstrom et al, 1978).…”

Section: Discussionmentioning

confidence: 98%

“…The definition of TSTA is still an operational one since it is based on the fact that immunization of syngeneic mice with a particular fibrosarcoma elicits transplantation immunity to that tumor but not to other similarly induced syngeneic neoplasms. Little is know of the biochemical nature and genetic origin of the diversity of TSTA, and this slow progress is mainly due to the difficulty of demonstrating the in vitro individual specificity of cellular or humoral immune reaction to TSTA of chemically induced murine tumors (Bhatnagar et al, 1975;Forbes et al, 1975;De Leo et al, 1977;Burton and Warner, 1978;Carbone et al, 1981;Old, 1981). The lack of an in vitro reproducible assay to measure the immune response to TSTA has also hampered the knowledge of the type of immune lymphocytes involved in the in vivo rejection of these tumors in preimmunized hosts.…”

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confidence: 99%

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In vitro detection of cell‐mediated immunity to individual tumor‐specific antigens of chemically induced BALB/c fibrosarcomas

Carbone

Colombo

Sensi

et al. 1983

Intl Journal of Cancer

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In this study we have analyzed the in vitro cell-mediated cytotoxicity of immune peritoneal exudate cells (PEC), elicited in syngeneic mice against the MCA-induced, TSTA-bearing BALB/c fibrosarcoma CA-2, GI-17 and C-3. The 4 h 51Cr-release assay showed the immune PEC effectors to be specifically cytotoxic to fibrosarcoma used for the immunization, but not to other syngeneic MCA-induced tumors or normal fibroblasts. Cold target inhibition experiments on CA-2 cells confirmed the specificity of the reaction. When PEC, lymph-node and spleen cells from BALB/c anti-CA-2 mice were compared for anti-tumor activity, only PEC were found to kill tumor cells significantly. PEC effectors did not have a significant level of NK or NC activities since they were unable to destroy YAC-1 or WEHI-164 tumor cells. PEC anti-CA-2 were analyzed for the expression of T-cell markers by anti-Thy 1.2, anti-Ly 1.2 and Ly 2.2 monoclonal antibodies. Anti-tumor specific effector cells were identified as mature T cells since they were not adherent to plastic and showed Thy 1.2+, Ly 1.2- and Ly 2.2+ phenotypes. In addition, anti-H-2Kd but not anti-H-2Dd alloantiserum added to target cells, blocked CA-2 tumor lysis, thus supporting the conclusion that the T-cell response against TSTA is H-2 restricted.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

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In vitro detection of cell‐mediated immunity to individual tumor‐specific antigens of chemically induced BALB/c fibrosarcomas

Carbone

Colombo

Sensi

et al. 1983

Intl Journal of Cancer

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“…Murine cell lines used (with strain indicated) were the plasmacytomas NS-1/1 .Ag4.1 (NS-1) (BALB/c) [9] and C1 .I8 (C3H/He) [lo], the B-lymphoma WEHI-231.1 (BALB/ c x N Z B F,) [Ill, and the T-lymphomas TIKAUT (AKR) [12], EL-4.1 (C57B1/6) [13], STRij-4-3.2 (BALB/c) [I41 and BW5147TG'Oua' (AKR) [15].…”

Section: Cell Lines and Micementioning

confidence: 99%

Heterogeneous glycosylation of murine transferrin receptor subunits

Driel

Goding

1985

European Journal of Biochemistry

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The N-linked glycosylation of the murine receptor for transferrin has been investigated. Previously we have found that purified receptors appear as two bands after sodium dodecyl sulfate/polyacrylamide gel electrophoresis and Coomassie blue staining [van Driel, I. R., Stearne, P. A., Grego, B., Simpson, R. J. and Goding, J. W. (1984) J. Immunol. 133,3220 -32241. In the current report we show that the two bands are due to different glycosylation of individual receptor molecules. The receptors have three asparagines to which N-linked glycans can be added, but only two sites are glycosylated in all receptors. The level of glycosylation of the third site varies depending on cell line or tissue. Limited endoglycosidase digestion of mature receptors indicates that differential glycosylation probably occurs at only one particular asparagine residue. Possible mechanisms that could result in such a glycosylation pattern are discussed.The cell surface receptor for transferrin in mouse and man is a disulfide-linked dimeric glycoprotein with approximate monomeric M , of 90000-95000 [l-71. The structure and biosynthesis of the human receptor have previously been investigated [2,6]. These studies showed that the mature human receptors have three N-linked glycans, two of which are of the high-mannose type and the other is of the complex type [2, 71. All human receptor monomers appear to have the same number and classes of N-linked glycans and in all other respects examined seem identical. Thus, the human receptor is probably a homodimer.Recently we have purified murine transferrin receptors to apparent homogeneity by a one-step procedure based on its physiology [8]. We found that, in contrast to the human transferrin receptor, the murine receptor appeared as two closely spaced bands on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). In this report we have determined that the two species correspond to receptor molecules that have different numbers of N-linked oligosaccharides. We have investigated this differential glycosylation using a combination of two-dimensional gel electrophoresis and digestion with glycosidases. MATERIALS AND METHODS Cell lines and miceCells were maintained in exponential growth in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum.

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“…Tumor rejection assays , however, have almost invariably not identified common cross-reacting TATA. Several exceptions among chemically-induced neoplasms have been reported (Leffell and Coggin, 1977;Economu et al 1977;Burton and Warner, 1978;Fritze et al, 1976). Hellstrom et al (1979) have described both in vitro and in vivo crossreactivity among some chemically-induced BALB/c sarcomas; the in vivo-detected cross-reactivity, indicating the presence of common or shared TATA, was stated to be strongest against those sarcomas expressing murine leukemia virus (MuLV)-associated antigens; these are found on cell surfaces or within many transplantable neoplasms of the mouse.…”

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confidence: 99%

Changes in tumor‐specific antigen expression during passage in vitro and in vivo of newly derived methylcholanthrene‐induced sarcomas of BALB/c mice

Law¹

1980

Intl Journal of Cancer

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The characteristics of tumor antigens of the transplantation type, TATA, were studied in a series of methylcholanthrene-induced sarcomas in pedigreed BALB/cAN mice. Each of the six sarcomas exhibited unique TATA when assayed for tumor rejection in syngeneic hosts. In three instances, however, in sarcomas CI-I, CII-5 and CII-7, TATA activity was lost during early in vivo passages. This activity was reestablished invivo from the in vitro-passaged lines in CI-I and CII-7 and these sarcomas along with CI-3, CI-4 and CII-10 maintained a stable TATA phenotype throughout the 50 transplant passages; TATA was lost permanently, however, in CII-5. These results indicate a dimorphic nature of early-passage neoplasms. No cross-reacting antigens were detected among these sarcomas, including also Meth A, a sarcoma well characterized as to its membrane antigens; nor was any evidence obtained for the existence of alien (inappropriate) H-2 antigens employing tumor rejection assays in F1 hybrids of BALB/c with strains bearing several haplotypes and also with studies of H-2 serology. In three sarcomas, some evidence was obtained for the existence of alloantigens of the non-H-2 type since it was found that (BALB/c X DBA/2)F1 hybrids could not be immunized by respective sarcomas. These findings suggest the existence on these sarcomas of a tumor antigen that is expressed normally in DBA/2 mice, although no definitive evidence has been obtained. Assays for MuLV, for the viral structural proteins gp70 and p30 and for reverse transcriptase showed that three sarcomas were positive and three others, as well as Meth A, were negative. MuLV and its antigens had no influence on tumor rejection activity nor could the cross-reactivity observed in the radioisotopic footpad assay (IFP) be related to MuLV.

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In vitro induction of tumour-specific immunity V. Detection of common antigenic determinatnts of murine fibrosarcomas

Cited by 19 publications

References 33 publications

In vitro detection of cell‐mediated immunity to individual tumor‐specific antigens of chemically induced BALB/c fibrosarcomas

In vitro detection of cell‐mediated immunity to individual tumor‐specific antigens of chemically induced BALB/c fibrosarcomas

Heterogeneous glycosylation of murine transferrin receptor subunits

Changes in tumor‐specific antigen expression during passage in vitro and in vivo of newly derived methylcholanthrene‐induced sarcomas of BALB/c mice

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