In vitro Inhibition of Clinical Isolates of Otitis Media Pathogens by the Probiotic Streptococcus salivarius BLIS K12

Chen, Tzu‐Yu; Hale, John K.; Tagg, John; Jain, Rohit; Voss, Abigail L; Mills, Nikki; Best, Emma; Stevenson, Duncan Scott; Bird, Philip; Walls, Tony

doi:10.1007/s12602-020-09719-7

Cited by 8 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…and C.J. Hinnebusch, 2005 [ 35 ] 23-year-old, male Right tympanic membrane retracted with a middle ear effusion Middle ear effusion Smear, culture, API Coryne system, and biochemicals Augmentin 20 C. Loiez et al, 2002 [ 34 ] 10-year-old, male Acute lymphoblastic leukemia B Blood culture and ear swab API Coryne system, culture, CAMP test Oral amoxicillin 21 Dana, A., R. Fader, and D. Sterken, 2001 [ 2 ] 5-year-old, female Bilateral ear pain Right and left middle Gram Stain, API Coryne, culture Intravenous cefotaxime 22 Reynolds, S.J., M. Behr, and J. McDonald, 2001 [ 3 ] 3-year-old, female Pain and swelling behind her right ear Ear PCR, culture, CAMP test, High-performance liquid chromatography analysis Intravenous cefuroxime and cloxacillin 23 Fernandez Perez, A., et al 1996 [ 13 ] 7-year-old, male Upper right neck pain radiating to the mastoid region Cervical abscess API Coryne and culture NS 24 Renaud, F.N., et al 1996 [ 1 ] 6-month-old, female Bilateral maxillolabiopalatine cleft with mucopurulent discharge Ear API Coryne, culture and biochemicals Amoxicillin-clavulanic acid …”

Section: Resultsmentioning

confidence: 99%

Metabolism and gene sequence variation in Turicella otitidis implies its adaptability and pathogenicity in extra-otic infection: a systematic review

Ahamad,

Yuan,

Chung

et al. 2023

BMC Infect Dis

View full text Add to dashboard Cite

Turicella otitidis belongs to the Corynebacteriaceae family and is a normal inhabitant of the ear and exists in a commensal relationship with its host. In children, T. otitidis is frequently associated with otitis media. The emergence of Turicella otitidis as a pathogen is concerning, particularly due to the limited availability of data on its pathogenic properties. The objective of this study is to conduct a systematic review of T. otitidis infections occurring in both the ear and other anatomical sites, and to summarize the differences in metabolism and genome sequences between isolates obtained from the ear and blood.

show abstract

Section: Resultsmentioning

confidence: 99%

Metabolism and gene sequence variation in Turicella otitidis implies its adaptability and pathogenicity in extra-otic infection: a systematic review

Ahamad,

Yuan,

Chung

et al. 2023

BMC Infect Dis

View full text Add to dashboard Cite

show abstract

“… NT not tested * P. aeruginosa does not grow well neither incorporated into the agar nor under the anaerobic conditions used in this test, **Some deferred antagonism tests with S. salivarius K12 and L. plantarum 8P-A3 did not yield unequivocal results; therefore, results are not listed. Results of this test have been reported to be markedly media-dependent [ 61 ] …”

Section: Resultsmentioning

confidence: 99%

“…Summary of the results of conventional tests (line test, double layer agar test, membrane test, and deferred antagonism test)NT not tested *P. aeruginosa does not grow well neither incorporated into the agar nor under the anaerobic conditions used in this test, **Some deferred antagonism tests with S. salivarius K12 and L. plantarum 8P-A3 did not yield unequivocal results; therefore, results are not listed. Results of this test have been reported to be markedly media-dependent[61] Membrane tests with S. salivarius M18 and K12 against incorporated S. aureus in WC and BHI agar medium: a Agar plates after incubation before removing the membranes; b…”

mentioning

confidence: 99%

A New Approach to Harness Probiotics Against Common Bacterial Skin Pathogens: Towards Living Antimicrobials

Khalfallah

Gartzen

Möller

et al. 2021

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

In this study, the potential of certain lactic acid bacteria—classified as probiotics and known to be antimicrobially active against pathogens or food-poisoning microorganisms—was evaluated with respect to their activity against bacterial skin pathogens. The aim of the study was to develop a plaster/bandage for the application of inhibitory substances produced by these probiotics when applied to diseased skin. For this purpose, two Streptococcus salivarius strains and one Lactobacillus plantarum were tested for production of antimicrobials (bacteriocin-like substances) active against Gram-positive and Gram-negative pathogens using established methods. A newly designed membrane test ensured that the probiotics produce antimicrobials diffusible through membranes. Target organisms used were Cutibacterium acnes, Staphylococcus aureus, and Pseudomonas aeruginosa. Moreover, the L. plantarum 8P-A3 strain was tested against additional bacteria involved in skin disorders. The Lactobacillales used were active against all potential skin pathogens tested. These probiotics could be enclosed between polymer membranes—one tight, the other permeable for their products, preserved by vacuum drying, and reactivated after at least three months storage. Importantly, the reactivated pads containing the probiotics demonstrated antibacterial activity on agar plates against all pathogens tested. This suggests that the probiotic containing pads may be topically applied for the treatment of skin disorders without the need for a regular antibiotic treatment or as an adjunctive therapy.

show abstract

“…It has been shown to inhibit Solobacterium moorei, Eubacterium saburreum, Streptococcus anginosus, and Parvimonas micra (formerly Micromonas micros), which are involved in oral malodor [24,25]. It also has a beneficial effect on reducing otitis media [26]. S. salivarius K12 may play a protective role against oral candidiasis.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Activity of the Probiotic Strain Streptococcus salivarius K12 against Oral Potential Pathogens

et al. 2021

View full text Add to dashboard Cite

Oral probiotics are increasingly used in the harmonization of the oral microbiota in the prevention or therapy of various oral diseases. Investigation of the antimicrobial activity of the bacteriocinogenic strain Streptococcus salivarius K12 against oral pathogens shows promising results, not only in suppressing growth, but also in eliminating biofilm formation. Based on these findings, we decided to investigate the antimicrobial and antibiofilm activity of the neutralized cell-free supernatant (nCFS) of S. salivarius K12 at various concentrations against selected potential oral pathogens under in vitro conditions on polystyrene microtiter plates. The nCFS of S. salivarius K12 significantly reduced growth (p < 0.01) in Streptococcus mutans Clarke with increasing concentration from 15 to 60 mg/mL and also in Staphylococcus hominis 41/6 at a concentration of 60 mg/mL (p < 0.001). Biofilm formation significantly decreased (p < 0.001) in Schaalia odontolytica P10 at nCFS concentrations of 60 and 30 mg/mL. Biofilm inhibition (p < 0.001) was also observed in Enterobacter cloacae 4/2 at a concentration of 60 mg/mL. In Schaalia odontolytica P10 and Enterobacter cloacae 4/2, the nCFS had no effect on their growth.

show abstract

In vitro Inhibition of Clinical Isolates of Otitis Media Pathogens by the Probiotic Streptococcus salivarius BLIS K12

Cited by 8 publications

References 28 publications

Metabolism and gene sequence variation in Turicella otitidis implies its adaptability and pathogenicity in extra-otic infection: a systematic review

Metabolism and gene sequence variation in Turicella otitidis implies its adaptability and pathogenicity in extra-otic infection: a systematic review

A New Approach to Harness Probiotics Against Common Bacterial Skin Pathogens: Towards Living Antimicrobials

Antimicrobial and Antibiofilm Activity of the Probiotic Strain Streptococcus salivarius K12 against Oral Potential Pathogens

Contact Info

Product

Resources

About