In vitro inhibition of human erythrocyte glutathione reductase by some new organic nitrates

Heavy metal ion inhibition studies of human, sheep and fish α-carbonic anhydrases

“…Studies regarding influences of various substances on mammalian or organisms CAs have gained a great attention in the recent years [35][36][37][38] . For instance, in vitro effect of some heavy metals on enzymes, such as the gill, intestinal and liver ones from various fish species showed that these enzymes were significantly inhibited by heavy metal ions 22,[39][40][41] . In this study, hCA I, hCA II, dCA and sCA IV were purified from human erythrocytes, D. labrax liver and sheep kidney by a simple one step procedure using Sepharose 4B-tyrosine-sulfanilamide affinity column.…”

Susceptibility of cord blood antioxidant enzymes glutathione reductase, glutathione peroxidase and glutathione S-transferase to different antibiotics:in vitroapproach

“…These enzymes are cooperative in several aspects 32,33 . Our groups recently investigated the interaction of human erythrocyte GR enzyme with several types of analgesic, anaesthetic drugs and antibiotics as well as metal ions 5,[9][10][11][12][13][14][15] .…”

“…Preparation of 2' , 5'-ADP Sepharose-4B affinity column was previously described 9,11,13 . The dialyzed sample obtained previously was loaded onto the 2' , 5'-ADP Sepharose-4B affinity column and the column was washed with 25 ml of 0.1 M K-acetate + 0.1 M K-phosphate, pH 6, and 25 ml of 0.1 M K-acetate + 0.1 M K-phosphate, pH 7.85.…”

“…Active fractions were collected and dialyzed with equilibration buffer. All of the procedures were performed at 4°C 5,13 . After dried Sephadex G-200 (2 g) was used for a 165 ml column (2 × 50 cm) bed volume.…”

“…Some studies found either increases or decreases in mammalian enzyme activities, and the inhibitor or activator effects of some antibiotics have been rarely investigated. Nevertheless, a few recent investigations regarding the inhibitory actions of different type of drugs and chemicals on GR have been reported by our group [9][10][11][12][13][14][15] . Cefuroxime is a second-generation cephalosporin antibiotic whereas ceftriaxone, ceftizoxime and cefoperazone are third-generation cephalosporin antibiotics.…”