IN-VITRO INHIBITION OF TYPE 2 DIABETES KEY ENZYMES; EFFECT OF EXTRACTS AND SOLVENT-SOLVENT FRACTIONS OF DANIELLIA OLIVERI (ROLFE) HUTCH. & DALZIEL

Abstract

Daniellia oliveri is a tree species belonging to the subfamily Caesalpinioideae (Leguminosae), whose young leaves are used locally to manage type 2 diabetes in Nigeria. This study aims at evaluating the inhibitory potentials of its crude ethanolic extract (Do-C) and solvent-solvent fractions (n-hexane (Do-H), diethyl ether (Do-D), and ethyl acetate (Do-E)) obtained from Do-C on α-amylase, α-glucosidase activities in-vitro and 1,1-diphenyl-2picraylhydrazine (DPPH) radical scavenging activity using standard protocols. Our findings showed that the Do-C and its fractions had significant TFC and TPC composition. All fractions inhibited DPPH free radicals effectively, with Do-E having excellent inhibition when compared with BHT. In this study, Do-C and its solvent-solvent fractions (Do-D, Do-E, and Do-H) inhibited α-amylase and α-glucosidase in a dose-dependent pattern. However, compared to acarbose, the Do-E exhibited similar inhibitory activity against α-amylase (P≤ 0.05). Nevertheless, the Do-E (IC50 35.02 ± 1.22 µg/ml) and Do-H (IC50 31.28 ± 1.23 µg/ml) had the best inhibitory activity against α-glucosidase comparatively after acarbose (25.97 ± 0.96 µg/ml). The inhibitory potential of Do-E could be linked to its TFC and TPC. Therefore, ethyl acetate fraction obtained from the crude ethanolic extract of D. oliveri could effectively inhibit key enzymes linked to type 2 diabetes (α-amylase and α-glucosidase). Further studies recommended to isolate antidiabetic compounds present.

Cheminformatics identification of modulators of key carbohydrate-metabolizing enzymes from C. cujete for type-2 diabetes mellitus intervention

“…Inclusively, the antidiabetic property of the various parts (leaves, stem bark and roots) of the plant in various extracts (aqueous, hydroethanol, ethanol) have been investigated in both in vitro and in vivo models [ 26 – 30 ]. Ethanol extract and fractions (hexane, diethyl ether and ethyl acetate) in a study by Adeyemi et al [ 29 ] showed comparative inhibition with acarbose against alpha-amylase with a superior effect over the standard against alpha-glucosidase. Additionally, aqueous root extract at 250 mg/kg body weight (b.w.)…”