In vitro interactions between ectomycorrhizal fungi and ericaceous plants

Villarreal-Ruiz, Luis; Neri-Luna, Cecilia; Anderson, Ian C.; Alexander, Ian J.

doi:10.1007/s13199-012-0161-7

Cited by 27 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Instead, a true ErM fungal species has first to be isolated from surfacesterilized (i.e., contaminant-free) Ericaceae hair roots in pure culture and, subsequently, it has to form intracellular hyphal coils similar to those observed under natural conditions in a monoxenic culture, with an apparently healthy Ericaceae host, at reasonable levels of colonization, i.e., similar to those of already verified ErM isolates (that is, in a way fulfilling Koch's postulates; Koch 1878). We make this conservative suggestion in an attempt to reduce the number of cases where claims of new ericoid mycorrhizal fungi are debatable, especially when those belong to typical saprobes (Zhang et al 2009) and typical ectomycorrhizal fungi (Bougoure et al 2007;Villarreal-Ruiz et al 2012;cf. Vohník et al 2012a), or even DSE (Usuki and Narisawa 2005;cf.…”

Section: Discussionmentioning

confidence: 99%

Experimental evidence of ericoid mycorrhizal potential within Serendipitaceae (Sebacinales)

et al. 2016

View full text Add to dashboard Cite

The Sebacinales are a monophyletic group of ubiquitous hymenomycetous mycobionts which form ericoid and orchid mycorrhizae, ecto- and ectendomycorrhizae, and nonspecific root endophytic associations with a wide spectrum of plants. However, due to the complete lack of fungal isolates derived from Ericaceae roots, the Sebacinales ericoid mycorrhizal (ErM) potential has not yet been tested experimentally. Here, we report for the first time isolation of a serendipitoid (formerly Sebacinales Group B) mycobiont from Ericaceae which survived in pure culture for several years. This allowed us to test its ability to form ericoid mycorrhizae with an Ericaceae host in vitro, to describe its development and colonization pattern in host roots over time, and to compare its performance with typical ErM fungi and other serendipitoids derived from non-Ericaceae hosts. Out of ten serendipitoid isolates tested, eight intracellularly colonized Vaccinium hair roots, but only the Ericaceae-derived isolate repeatedly formed typical ericoid mycorrhiza morphologically identical to ericoid mycorrhiza commonly found in naturally colonized Ericaceae, but yet different from ericoid mycorrhiza formed in vitro by the prominent ascomycetous ErM fungus Rhizoscyphus ericae. One Orchidaceae-derived isolate repeatedly formed abundant hyaline intracellular microsclerotia morphologically identical to those occasionally found in naturally colonized Ericaceae, and an isolate of Serendipita (= Piriformospora) indica produced abundant intracellular chlamydospores typical of this species. Our results confirm for the first time experimentally that some Sebacinales can form ericoid mycorrhiza, point to their broad endophytic potential in Ericaceae hosts, and suggest possible ericoid mycorrhizal specificity in Serendipitaceae.

show abstract

Section: Discussionmentioning

confidence: 99%

Experimental evidence of ericoid mycorrhizal potential within Serendipitaceae (Sebacinales)

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Successful colonization was obtained with both inoculation treatments -(i) isolated liverwort-derived ErM strains in culture and (ii) intact leafy liverworts colonized with ErM fungiregardless of the plant's mode of propagation, that is seedling or cutting. Previously, fungal transfers had only been studied either from Ericaceae to Ericaceae seedlings in autoclaved substrates (Stribley, Read & Hunt 1975;Strandberg & Johansson 1999;Vosatka et al 1999;Jansa & Vosatka 2000) or in vitro (Upson, Read & Newsham 2007;Villarreal-Ruiz et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Vaccinium spp. experienced enhanced growth and nutrient uptake after 167 days by introducing ericoid mycorrhizal inoculum to sterilized peat compost (Litten, Smagula & Dalpe 1992), but a more recent study with the same plant genus reported equivocal results (Villarreal-Ruiz et al 2012). The fungus Meliniomyces bicolor (in the P. ericae species aggregate) enhanced root length by 2½ times while another fungal isolate related to P. ericae suppressed growth in some Vaccinium spp.…”

Section: Introductionmentioning

confidence: 99%

Liverworts to the rescue: an investigation of their efficacy as mycorrhizal inoculum for vascular plants

et al. 2015

View full text Add to dashboard Cite

Summary Pezoloma ericae (D.J. Read) Baral, a widespread mycorrhizal fungus of plants in the Ericales, is known to form intracellular associations with several families of leafy liverworts (Schistochilaceae, Lepidoziaceae, Cephaloziaceae, Cephaloziellaceae) in vitro. The ecological significance of this link between vascular and non‐vascular plants is unknown. Fungal symbionts were isolated from rhizoids of the leafy liverworts Cephalozia connivens (Dicks.) Lindb. and C. bicuspidata (L.) Dum. (Cephaloziaceae), as well as from the hair roots of two dominant ericoid mycorrhiza‐forming species of European heathlands, Erica tetralix (L.) and Calluna vulgaris (L.). Using pure cultures of P. ericae, we resynthesized liverwort–fungus associations to use colonized liverworts as inoculum which was applied to substrates supporting the growth of heather seedlings and cuttings. Effects were quantified using germination, rooting, plant colonization, plant survival under waterlogging stress and growth in height in experimental systems with and without liverworts and/or fungi. Fungal symbionts growing from liverwort rhizoids readily colonized the hair roots of ericaceous plants to form typical ericoid mycorrhizas. The presence of inoculum‐bearing liverworts led to significant increases in plant growth. Erica tetralix was more responsive to inoculation than C. vulgaris. Ericaceous cuttings rooted and survived more successfully when they were coplanted with previously colonized liverwort stems. We demonstrate, under realistic ecological circumstances, that liverworts can deliver mycorrhizal inoculum and improve the establishment of vascular plants. We propose that by providing sources of mycorrhizal inoculum, symbiotic non‐vascular plants can contribute to the restoration of plant communities dominated by Ericales plants. This research leads to broader knowledge about the function of ericoid mycorrhizas in ecosystems.

show abstract

“…as part of an EcM chronosequence pilot study at Glen Tanar Nature Reserve, Aberdeenshire, NE Scotland, UK (National Grid Reference No. 470950, see Villarreal-Ruiz 2006), Figure 1. Because it was difficult to locate a 50-year-old naturally regenerated stand in order to construct the full chronosequence, the problem was overcome by selecting a large plantation within the forest area that met most of Oliver’s (1982) selection criteria.…”

Section: Methodsmentioning

confidence: 98%

“…Ectomycorrhizal (EcM) fungi are species-rich communities playing a central role in ecosystem processes in boreal forests and native pinewoods (Dahlberg 2001; Lindea van der et al 2010; Tedersoo et al 2014). They establish complex bipartite and tripartite mutualistic networks with overstorey and understorey plants allowing them to share nutrient resources and relaxing the competition (Villarreal-Ruiz et al 2004, 2012; Fodor 2013). Estimations of fungal species richness in Scotland suggest that 8000–15,000 taxa are present and between 35 and 40% are mycorrhizal, mainly based on above-ground sporocarp presence (Watling 1997; Iskipp 2000).…”

Section: Introductionmentioning

confidence: 99%

Testing sampling effort and relative abundance descriptors of belowground ectomycorrhizal fungi in a UK planted scots pine woodland

Villarreal-Ruiz

Neri-Luna

2017

Mycology

Self Cite

View full text Add to dashboard Cite

The native Caledonian pinewoods of Scotland, UK contain a unique and unexplored biodiversity of below-ground ectomycorrhizal fungi which may constitute a valuable source of microbial genetics resources for woodland restoration. In this study we test the sampling effort, taxa detection (ectomycorrhiza morpho-anatotyping) and relative abundance measurements (ectomycorrhizal root tip number vs. dry weight) of below-ground ectomycorrhizal fungal communities in a planted Pinus sylvestris Caledonian woodland. A total of 18 replicated sampling points were set up at differential distance along a 125 m transect, in a 50-yr-old, stem exclusion: thicket phase, Pinus sylvestris pinewood stand. A total of 11 ectomycorrhizal morpho-anatotypes were detected from 6689 ectomycorrhizal root tips counted and weighing 992.6 mg dry weight. The major ectomycorrhizal taxa were Meliniomyces bicolor and Cortinarius spp. accounting ~50% of total pine roots. A highly significant relationship (r2 = 0.16, p < 0.000) was found between Sørensen dissimilarity in soil cores and distance apart. In this study, the spatial arrangement of samples indicated that over short distances the dissimilarity was lower in contrast with the longer distances along the transect.

show abstract

In vitro interactions between ectomycorrhizal fungi and ericaceous plants

Cited by 27 publications

References 36 publications

Experimental evidence of ericoid mycorrhizal potential within Serendipitaceae (Sebacinales)

Experimental evidence of ericoid mycorrhizal potential within Serendipitaceae (Sebacinales)

Liverworts to the rescue: an investigation of their efficacy as mycorrhizal inoculum for vascular plants

Testing sampling effort and relative abundance descriptors of belowground ectomycorrhizal fungi in a UK planted scots pine woodland

Contact Info

Product

Resources

About