In vitro Investigation of Blood Compatibility of Ti with Oxide Layers of Rutile Structure

Huang, Nan; Chen, Y R; Luo, J.M.; Yi, Jin; Lu, Ruowei; Xiao, Jianlin; Xue, Zhiyuan; Liu, Xionghua

doi:10.1177/088532829400800406

Cited by 105 publications

(52 citation statements)

References 9 publications

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“…Rutile titanium oxide ceramics, TiO 2 and TiO x films prepared by thermal oxidation and ion beamassisted deposition (IBAD) [325][326][327][328][329] generally have blood compatibility better than that of clinically biomaterials, such as LTIC. Hence, titanium oxide is potential a good blood-contacting biomaterial.…”

Section: Oxygen Implantationmentioning

confidence: 99%

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

Liu

Chu

Ding

2004

Materials Science and Engineering: R: Reports

3,079

1,477

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Titanium and titanium alloys are widely used in biomedical devices and components, especially as hard tissue replacements as well as in cardiac and cardiovascular applications, because of their desirable properties, such as relatively low modulus, good fatigue strength, formability, machinability, corrosion resistance, and biocompatibility. However, titanium and its alloys cannot meet all of the clinical requirements. Therefore, in order to improve the biological, chemical, and mechanical properties, surface modification is often performed. This article reviews the various surface modification technologies pertaining to titanium and titanium alloys including mechanical treatment, thermal spraying, sol-gel, chemical and electrochemical treatment, and ion implantation from the perspective of biomedical engineering. Recent work has shown that the wear resistance, corrosion resistance, and biological properties of titanium and titanium alloys can be improved selectively using the appropriate surface treatment techniques while the desirable bulk attributes of the materials are retained. The proper surface treatment expands the use of titanium and titanium alloys in the biomedical fields. Some of the recent applications are also discussed in this paper. #

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Section: Oxygen Implantationmentioning

confidence: 99%

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

Liu

Chu

Ding

2004

Materials Science and Engineering: R: Reports

3,079

1,477

View full text Add to dashboard Cite

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“…In our study, both HUVEC and EPC were used for coating DHUV with the objective of producing a confluent, functional antithrombotic surface. HUVEC are mature EC that line HUV whereas, EPC can be differentiated into EC and easily be harvested from peripheral blood of patients [38,49]. Generation of an engineered vascular graft usually takes more than 8 weeks.…”

Section: Discussionmentioning

confidence: 99%

“…The change in the optical density of the solution versus time was plotted. Clotting times were estimated for all test materials, including the tubes, non-seeded HUV, cell-seeded HUV and cellseeded HUV with S1P treatment as previously described [38,39].…”

Section: Coagulation and Kinetic Clotting Testsmentioning

confidence: 99%

Sphingosine-1-phosphate improves endothelialization with reduction of thrombosis in recellularized human umbilical vein graft by inhibiting syndecan-1 shedding in vitro

et al. 2017

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a b s t r a c tSphingosine-1-phosphate (S1P) has been known to promote endothelial cell (EC) proliferation and protect Syndecan-1 (SDC1) from shedding, thereby maintaining this antithrombotic signal. In the present study, we investigated the effect of S1P in the construction of a functional tissue-engineered blood vessel by using human endothelial cells and decellularized human umbilical vein (DHUV) scaffolds. Both human umbilical vein endothelial cells (HUVEC) and human cord blood derived endothelial progenitor cells (EPC) were seeded onto the scaffold with or without the S1P treatment. The efficacy of recellularization was determined by using the fluorescent marker CellTracker CMFDA and anti-CD31 immunostaining. The antithrombotic effect of S1P was examined by the anti-aggregation tests measuring platelet adherence and clotting time. Finally, we altered the expression of SDC1, a major glycocalyx protein on the endothelial cell surface, using MMP-7 digestion to explore its role using platelet adhesion tests in vitro. The result showed that S1P enhanced the attachment of HUVEC and EPC. Based on the anti-aggregation tests, S1P-treated HUVEC recellularized vessels when grafted showed reduced thrombus formation compared to controls. Our results also identified reduced SDC1 shedding from HUVEC responsible for inhibition of platelet adherence. However, no significant antithrombogenic effect of S1P was observed on EPC. In conclusion, S1P is an effective agent capable of decreasing thrombotic risk in engineered blood vessel grafts. Statement of SignificanceSphingosine-1phosphate (S1P) is a low molecular-weight phospholipid mediator that regulates diverse biological activities of endothelial cell, including survival, proliferation, cell barrier integrity, and also influences the development of the vascular system. Based on these characters, we the first time to use it as an additive during the process of a small caliber blood vessel construction by decellularized human umbilical vein and endothelial cell/endothelial progenitor. We further explored the function and Contents lists available at ScienceDirect Acta Biomaterialia j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l oc a t e / a c t a b i o m a t mechanism of S1P in promoting revascularization and protection against thrombosis in this tissue engineered vascular grafts. The results showed that S1P could not only accelerate the generation but also reduce thrombus formation of small caliber blood vessel.

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“…maintenance of tissue structure, wound healing, immune response, metastasis, and tissue integration of biomaterials 18,19) . It was reported that cell spreading could be divided into three stages shown in and transforms to fibrin monomer, it will quickly cross-link to irreversible thrombus 22) . TiO 2 film has higher dielectric constant and is semiconductive, so it can inhibit the electron transfer, prevent fibrinogen from denaturing and decrease thrombus formation 23) .…”

Section: Methodsmentioning

confidence: 99%

L929 Cell Adhesion on the Surface Oxidized NiTi Shape Memory Alloy

Ying¹,

Wang²,

Nakano³

et al. 2011

J. Hard Tissue Biology.

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Oxide film was obtained on a NiTi shape memory alloy by a chemical method. In vitro bioactivity of the oxidized NiTi shape memory alloy was studied. Cell culture showed that both non-oxidized and oxidized NiTi shape memory alloy did not have cell toxicity while the oxidized displayed higher cell viability. More L929 cells were found to adhere and proliferate on the oxidized samples surface than that on the non-oxidized. Cell number increased slowly for the test alloy samples'. Oxidized NiTi shape memory alloy possesses equal cellactivating properties to the pure titanium. More intercellular substance was observed for the cells on the oxidized NiTi alloy. The oxidized NiTi alloy shows better cell response than the non-oxidized. Surface oxidation improves the bioactivity of NiTi shape memory alloy.

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In vitro Investigation of Blood Compatibility of Ti with Oxide Layers of Rutile Structure

Cited by 105 publications

References 9 publications

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

Sphingosine-1-phosphate improves endothelialization with reduction of thrombosis in recellularized human umbilical vein graft by inhibiting syndecan-1 shedding in vitro

L929 Cell Adhesion on the Surface Oxidized NiTi Shape Memory Alloy

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