In vitro investigation of intestinal transport mechanism of silicon, supplied as orthosilicic acid‐vanillin complex

Sergent, Thérèse; Croizet, Karine; Schneider, Yves‐Jacques

doi:10.1002/mnfr.201600602

Cited by 4 publications

(5 citation statements)

References 23 publications

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“…After 3 h, more than 55% of the silicon crossed the Caco-2 monolayer. It is already known that mesoporous silica can dissolve at pH between 7 and 10 [62] and that silicon crosses the Caco-2 monolayer via paracellular transport [51]. What remains to be determined is if silicon can interact with the CYP1A2 enzyme and modify its activity.…”

Section: Discussionmentioning

confidence: 99%

“…Figure 5b shows that the percentage of mLT that is converted into 6-HMLT in comparison to the mLT absorbed through the Caco-2 monolayer for pure mLT and AMS-6/MLT is different during the first hour but not after. The difference present in the first hour can be due to the dissolution of silica in the buffer, transport of silicic acid to the basolateral side [51] and the interaction of silicic acid with the hydroxylation enzyme. In the first 2 h, the percentage of mLT converted into 6-HMLT in comparison to the mLT absorbed is significantly higher for AMS-6/MLT:CAP(1:1) and (1:2) than for the other samples.…”

Section: Hydroxylation Of Mltmentioning

confidence: 99%

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Effects of Absorption Kinetics on the Catabolism of Melatonin Released from CAP-Coated Mesoporous Silica Drug Delivery Vehicles

Moroni

Garcia‐Bennett

2021

Pharmaceutics

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Melatonin (MLT) is a pineal hormone involved in the regulation of the sleep/wake cycle. The efficacy of exogenous MLT for the treatment of circadian and sleep disorders is variable due to a strong liver metabolism effect. In this work, MLT is encapsulated in mesoporous silica (AMS-6) with a loading capacity of 28.8 wt%, and the mesopores are blocked using a coating of cellulose acetate phthalate (CAP) at 1:1 and 1:2 AMS-6/MLT:CAP ratios. The release kinetics of MLT from the formulations is studied in simulated gastrointestinal fluids. The permeability of the MLT released from the formulations and its 6-hydroxylation are studied in an in vitro model of the intestinal tract (Caco-2 cells monolayer). The release of MLT from AMS-6/MLT:CAP 1:2 is significantly delayed in acidic environments up to 40 min, while remaining unaffected in neutral environments. The presence of CAP decreases the absorption of melatonin and increases its catabolism into 6-hydroxylation by the cytochrome P450 enzyme CYP1A2. The simple confinement of melatonin into AMS-6 pores slightly affects the permeability and significantly decreases melatonin 6-hydroxylation. Measurable amounts of silicon in the basolateral side of the Caco-2 cell monolayer might suggest the dissolution of AMS-6 during the experiment.

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Section: Discussionmentioning

confidence: 99%

Section: Hydroxylation Of Mltmentioning

confidence: 99%

Effects of Absorption Kinetics on the Catabolism of Melatonin Released from CAP-Coated Mesoporous Silica Drug Delivery Vehicles

Moroni

Garcia‐Bennett

2021

Pharmaceutics

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“…The daily intake for humans ranges from 9000 – 30 000 µg silicon 4 , 7 , but official reference values do not yet exist 8 . Silicon resorption by the gastrointestinal tract takes place by passive diffusion through intercellular tight junctions and presumably by facilitated diffusion 9 . After oral intake.…”

Section: Introductionmentioning

confidence: 99%

“…Silicon Resorption from Equisetum arvense Tea -A Randomized, Three-Armed Pilot Study cellular tight junctions and presumably by facilitated diffusion [9]. After oral intake.…”

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confidence: 99%

Silicon Resorption from Equisetum arvense Tea – A Randomized, Three-Armed Pilot Study

et al. 2021

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Equisetum arvense tea (TEA) contains high concentrations of silicon and has been used in folk medicine for the treatment of inflammatory ailments. We examined the resorption of silicon after TEA consumption. Safety and immunological effects were secondary outcomes. A monocentric, randomized, three-armed pilot study was conducted with 12 voluntary, healthy, male subjects. The study is registered in the German register for clinical trials (DRKS-ID: DRKS00016628). After a low silicon diet for 36 hours, 1000 mL TEA1 with approximately 200 000 µg silicon/L, TEA2 with approximately 750 000 µg silicon/L, or Si-low-Water (approximately 10 – 10 000 µg silicon/L as a control) were ingested on three consecutive days. Blood and urine samples were collected at baseline, day 1 examining silicon kinetics, day 3 examining silicon accumulation, and day 8 (safety, immunological parameters). Si-low-Water intake did not change silicon serum (Cmax 294 µg/L) or urine (19 000 µg/24 h) concentrations compared to baseline. Cmax was 2855 µg/L for TEA1 and 2498 µg/L for TEA2; tmax was 60 and 120 min, respectively. Silicon accumulation did not occur. Urine silica within 24 h (E24 h) was higher after TEA2 compared to TEA1 ingestion (142 000 vs. 109 000 µg/24 h). Serum silicon levels at t = 120 min differed significantly after intake of TEA2 or intake of Si-low-Water (p = 0.029). The immunological parameters did not show any significant changes indicating immunosuppressive effects in volunteers. TEA1 was well tolerated, while TEA2 caused diarrhoea in 4 subjects. Our investigations show that intake of TEA1 leads to significant rise in serum silicon concentration.

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“…In animals, silicon (Si) is ubiquitously distributed and found mainly in the form of orthosilicic acid (H 4 SiO 4 ). It is also in this form that Si is ingested from many environmental sources, is absorbed by the intestinal epithelium and is excreted in urine (Jugdaohsingh et al, ; Sergent, Croizet, & Schneider, ). Of importance, Si is particularly abundant in cartilage, aorta, bone, and skin where it is bound to a variety of organic matrices (Carlisle, ; Landis, Lee, Brenna, Chandra, & Morrison, ).…”

Section: Introductionmentioning

confidence: 99%

A new gold standard approach to characterize the transport of Si across cell membranes in animals

Garneau

Marcoux

Frenette‐Cotton

et al. 2018

Journal Cellular Physiology

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Silicon (Si) is increasingly recognized as an essential trace element in animals, especially since the identification of mammalian Si transport systems and Si responsive genes not long ago. During many years, however, efforts to gain substantial insight into the biological role of this element in animals have achieved partial success due in part to the unavailability of validated protocols to study Si movement across biological membranes. To circumvent such limitations, we have developed a general transport assay in which cellular Si content was determined by automated electrothermal atomic absorption spectrophotometry. We have found this assay to provide great analytic sensitivity with Si detection thresholds of less than 1 µM, that is, below or very close to the concentration range of animal cells. We have also found this assay to provide valid and cost-effective determinations in Si transport studies while requiring workable quantities of samples. The protocol described here should thus become gold standard toward accelerated progress in the field of Si transport.

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In vitro investigation of intestinal transport mechanism of silicon, supplied as orthosilicic acid‐vanillin complex

Cited by 4 publications

References 23 publications

Effects of Absorption Kinetics on the Catabolism of Melatonin Released from CAP-Coated Mesoporous Silica Drug Delivery Vehicles

Effects of Absorption Kinetics on the Catabolism of Melatonin Released from CAP-Coated Mesoporous Silica Drug Delivery Vehicles

Silicon Resorption from Equisetum arvense Tea – A Randomized, Three-Armed Pilot Study

A new gold standard approach to characterize the transport of Si across cell membranes in animals

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