In Vitro Investigation of the Anticancer Properties of Ammodaucus Leucotrichus Coss. & Dur.

Abstract

Little is known about the pharmacological activity of Ammodaucus leucotrichus Coss. & Dur., a small annual species that grows in the Saharan and sub-Saharan countries. In the present study, we investigated whether the standardized ethanolic extract of A. leucotrichus fruits and R-perillaldehyde, a monoterpenoid isolated from A. leucotrichus fruits, are able to affect different processes involved in different phases of cancer development. In particular, we explored their genoprotective, proapoptotic, antiproliferative, and cytodifferentiating potential on different human cell models. We analyzed the genoprotective and proapoptotic activity on human lymphoblast cells (TK6) using the micronucleus test, and the cytodifferentiation effects on human promyelocytic cells (HL60) through the evaluation of different markers of differentiation forward granulocytes or monocytes. The results showed that the extract and perillaldehyde were able to induce apoptosis and protect from clastogen-induced DNA damage. To our best knowledge, this is the first report on the ability of A. leucotrichus and perillaldehyde to induce apoptosis and protect DNA from the toxicity of different compounds. Data reported in this work are the starting point for their pharmacological use. Going forward, efforts to determine their effects on other events associated with cancer development, such as angiogenesis and metastasization, will provide important information and improve our understanding of their potential in cancer therapy.

The Bioactive Properties of Carotenoids from Lipophilic Sea buckthorn Extract (Hippophae rhamnoides L.) in Breast Cancer Cell Lines

“…Many in vitro studies evaluate the anticancer properties of plant-based bioactive compounds using one [51,52] or more different cell lines [53,54] for a certain cancer pathology and at least three biological replicates (n = 3). The relevance of our study was also confirmed by the selected methods in order to evaluate the anticancer properties at the cellular level as follows: the Alamar blue assay [55] used to test the cytotoxic and antiproliferative effect of a compound is not cytotoxic and is more sensitive than tetrazolium assays (e.g., MTT, XTT, WST-8), while the apoptosis assay through flow cytometry [56] is more accurate when determining the precise number of apoptotic cell populations than immunocytochemistry where cells may suffer significant damage from the paraformaldehyde used in the fixation protocol. Although our study selected two different cell lines with different phenotypes and performed the experiments in three biological replicates (n = 3), the study should be extended to more breast cancer cell lines with different phenotypes as well to healthy cells, in order to prove the cytotoxic selectivity and proapoptotic properties of LSBE.…”

Antimutagenicity and Antioxidant Activity of Castanea sativa Mill. Bark Extract

“…Cytotoxicity was measured in samples treated with the following Cs bark extract concentrations: 0, 3, 6, 12, 18, 24, 48 µg/mL. Cytotoxicity was measured using the Guava ViaCount Reagent and performing Guava ViaCount Assay, according to the manufacturer's instructions and previous publications [24][25][26]. In total, 1000 events were acquired.…”

“…After the treatment, the Mni frequency was assessed by a flow cytometry (FCM) according to the protocol published by Lenzi et al [32] and already used in previous publications [22,25,31]. The Mni frequency was normalized to that of the untreated control, considered 1 and reported as the Mni frequency fold increase.…”

Improvement of the antioxidant potential: impact of drying and extraction techniques on polyphenols in Arbutus unedo L. leaf aqueous extract

“…Medicinal plants have an important place in therapeutic and cosmetic uses worldwide (Zemour et al, 2019 ;Labdeli et al, 2019;El Zerey-Belaskri et al, 2022;Sousa et al, 2022;Lenzi et al, 2022). Throughout history, various cultures and traditional systems of medicine have relied on the healing properties of plants to treat illnesses and promote well-being.…”