1992
DOI: 10.1016/s0021-9258(18)42466-0
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In vitro isoprenylation and membrane association of mouse rod photoreceptor cGMP phosphodiesterase alpha and beta subunits expressed in bacteria.

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Cited by 72 publications
(7 citation statements)
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“…The catalytic subunits of the PDE, R and β, undergo posttranslational C-terminal prenylation. The R subunit has been shown to be farnesylated on the cysteine of the C-terminal motif CCVQ, whereas the β subunit has been shown to be geranylgeranylated on the cysteine of CRIL (3,4), consistent with the specificity rules for protein prenyltransferases (5,6). Some evidence suggests that the C-termini of the PDE catalytic subunits are required in order for the PDE to remain membrane-bound.…”
mentioning
confidence: 78%
“…The catalytic subunits of the PDE, R and β, undergo posttranslational C-terminal prenylation. The R subunit has been shown to be farnesylated on the cysteine of the C-terminal motif CCVQ, whereas the β subunit has been shown to be geranylgeranylated on the cysteine of CRIL (3,4), consistent with the specificity rules for protein prenyltransferases (5,6). Some evidence suggests that the C-termini of the PDE catalytic subunits are required in order for the PDE to remain membrane-bound.…”
mentioning
confidence: 78%
“…Specific receptors may be involved in localizing some peripheral membrane proteins, such as p60src (Resh, 1989;Resh & Ling, 1990), to specific membranes and may recognize lipids covalently attached to these proteins. However, if the isoprenyl groups on PDEa|s (Anant et al, 1992) or the N-linked myristic acid that has recently been found on Ta (Wensel & Yang, 1992) is important for these proteins to bind membranes, as has been suggested for PDE (Ong et al, 1989;Catty & Deterre, 1991;Qin et al, 1992), their contributions to membrane binding clearly do not require specific receptor proteins. Nor do membranes enhance activation by serving as protein concentrators.…”
Section: P-i-t-mmentioning
confidence: 97%
“…PDE is bound through the isoprenylated carboxy termini of the catalytic a and p subunits to rod disc membranes (Qin, Pittler, and Baehr, 1992;Anant et al, 1992). The isoprenylated membrane-bound PDE is recognised and solubilised by PDE5 under physiological conditions .…”
Section: 61viii -Cone Pigmentsmentioning
confidence: 99%