In vitro leishmanicidal activity of two cholesterol derivatives

Isaac-Márquez, Angélica Patricia; Lezama-Dávila, Claudio M.

doi:10.1007/s11274-022-03248-x

Cited by 1 publication

(1 citation statement)

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“…Infected macrophages were fixed with methanol and stained with Giemsa (20% in PBS). The anti-Leishmania activity was determined by counting the number of parasites per 100 macrophages (Lezama-Dávila et al 2014;Isaac-Márquez and Lezama-Dávila 2022), which was used to record the IC50 of the extracts on amastigote populations calculated using program GraphPad Prism v.8. All samples were tested in triplicate.…”

Section: B) On Amastigotesmentioning

confidence: 99%

Anti- Leishmania activity of the Mayan medicinal plant Thouinia paucidentata Radlk extracts

Uc-Cachón

Chan-Bacab

Lezama-Dávila

et al. 2023

Natural Product Research

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Mexico's Yucatan Peninsula is an endemic area of cutaneous leishmaniasis, locally known as the chiclero´s ulcer, and Mayan traditional medicine which refers to the use of Thouinia paucidentata Radlk, known as k'an chuunup. Aqueous and organic leaves extracts were evaluated against promastigotes and amastigotes of Leishmania mexicana. Toxicity tests of extracts were performed using Vero and J774A.1 macrophage cell lines. The composition of the most active extracts was analyzed by GC-MS. The n-hexane and ethyl acetate extracts showed potent anti-Leishmania activity against the promastigote form, and remarkably, nhexane extract exhibited potent activity against the amastigote form. Both extracts showed low toxicity on Vero both not on J774A.1 cells. Analysis of both bioactive extracts identified as more abundant compounds, germacrene D-4-ol and thunbergen in n-hexane, and thunbergol in ethyl acetate extracts. Our study presents T. paucidentata as anti-Leishmania phytomedicine supporting its medicinal use and contributes to the understanding of its phytochemical composition.

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Section: B) On Amastigotesmentioning

confidence: 99%