In vitro lipid metabolism in the rat pancreas I. Basal lipid metabolism

Abstract

1. The in vitro basal lipid metabolism of rat pancreatic fragments was compared with that in adipose tissue fragments and liver slices. 2. [1-14C]Acetate added to the media was mostly incorporated into palmitic acid and to a lesser extent into oleic acid. In addition, pancreatic tissue exhibited a marked capacity for elongation of polyunsaturated fatty acids by [1-14C]acetate and resulting desaturation when compared to adipose tissue and liver. 3. Data obtained in the presence of [U-14C]glucose, [1-14C]palmitate and 3H20 indicate that acetyl-CoA derived from glucose and from beta-oxidation of fatty acids contributed to de novo lipogenesis. 4. Oxidation of [1-14C]palmitic acid was 9-13 times higher in the pancreas than in adipose tissue or liver when expressed on a wet weight basis. 5. The fatty acid moiety of pancreatic glycerolipids could be derived from de novo synthesis, fatty acids added to the medium, or from fatty acids formed from the hydrolysis of endogenous lipids. The glycerol moiety could be derived either from glucose, or directly from glycerol through participation of glycerol kinase.

Metabolism addiction in pancreatic cancer

“…First, normal pancreatic cells have been shown to utilize lipids as an energy source (as determined by oxidation of palmitic acid) at higher rates than other cell types (e.g., cells of adipose or liver tissue). 114 Thus, the proliferative effect of lipids added as a supplement to the growth medium of pancreatic cancer cells may imply that lipids provide an energy source for pancreatic cell lines. Second, enhancement of cell proliferation by hormones and growth factors is known to be coupled with generation of lipids and lipid-derived messengers that participate in the activation of different growth-regulatory pathways.…”

The interaction of lithium with thyrotropin-releasing hormone-stimulated lipid metabolism in GH3 pituitary tumour cells. Enhancement of stimulated 1,2-diacylglycerol formation

“…Neutral lipids were separated on activated silica gel G t.l.c. plates by the method of Calderon et al (1979). All other phospholipids were separated by two-dimensional t.l.c.…”

Prostaglandin F2α stimulates phosphatidylinositol turnover and increases the cellular content of 1,2‐diacylglycerol in confluent resting swiss 3T3 cells

“…The pellet was finally resuspended in 0.5 ml distilled water and neutral lipids extracted by the method of Schacht (1981). Triacylglycerol, 1,3-diacylglycerol, 1,2-diacylglycerol, and monacylglycerol were separated by thin-layer chromatography on Silica Gel-G plates (20 cm x 20 cm; 0.25 mm thick) using the method of Calderon et al (1979). Lipids were detected using iodine vapor, the bands corresponding to triacylglycerol and 1,2-diacylglycerol were scraped, and the incorporated radioactivity was determined by liquid scintillation counting.…”