In vitro maintenance of Eperythrozoon suis

Proposal to transfer some members of the genera Haemobartonella and Eperythrozoon to the genus Mycoplasma with descriptions of 'Candidatus Mycoplasma haemofelis', 'Candidatus Mycoplasma haemomuris', 'Candidatus Mycoplasma haemosuis' and 'Candidatus Mycoplasma wenyonii'.

“…Little is known about the metabolism of these uncultivated mycoplasmas but Smith et al (1990) observed that glucose consumption by red cells infected by Eperythrozoon suis (' Candidatus Mycoplasma haemosuis ') is inhibited by iodoacetate and by sodium fluoride, and suggested that this bacterium is responsible for the glucose consumption and that it metabolizes glucose by glycolysis ; lactate may be the major end product of glycolysis (Nonaka et al, 1996). This metabolic pattern would be consistent with that of fermentative mollicutes (Neimark, 1979).…”

“…DNA from organisms in blood is well preserved on FTA blood cards (Fitzco) (H. Neimark & M. Ganter, unpublished). Infected blood has been cryopreserved by adding 40 % polyvinyl pyrrolidone (6n7 % final concn)\10 % glycerol and freezing in liquid nitrogen (Nonaka et al, 1996) ; dimethylsulfoxide also has been used for cryopreservation but 10 % glycerol\10 % horse serum commonly used for preserving mycoplasmas at k70 mC may be adequate. The conditions for in vitro maintenance of ' Candidatus Mycoplasma haemosuis ' described by Nonaka et al (1996) probably would be useful for other haemotrophic mycoplasmas and information about mycoplasma physiology and genetics should be applicable as well.…”

Mycoplasma suisAntigens Recognized during Humoral Immune Response in Experimentally Infected Pigs

“…Chronic low-grade M. suis infections vary from asymptomatic infections to a range of clinical conditions, including (i) anemia, mild icterus, and general unthriftiness in newborns, (ii) growth retardation in feeder pigs, and (iii) poor reproductive performance in sows (3,8,34). Moreover, M. suis is suspected of suppressing the host's immune response, leading to an increased proneness to infection with other infectious agents of porcine respiratory and enteric diseases (33).The lack of an in vitro cultivation system is the crucial barrier to systematic analyses of the biology of M. suis as well as the development of valuable diagnostic procedures for, e.g., the accurate assessment of the prevalence and significance of M. suis in pig populations (5,20). Hitherto, laboratory diagnosis of M. suis usually relies on the microscopic examination of chemically stained peripheral blood smears to directly visualize the microorganisms attached to erythrocytes (15).…”

“…The lack of an in vitro cultivation system is the crucial barrier to systematic analyses of the biology of M. suis as well as the development of valuable diagnostic procedures for, e.g., the accurate assessment of the prevalence and significance of M. suis in pig populations (5,20). Hitherto, laboratory diagnosis of M. suis usually relies on the microscopic examination of chemically stained peripheral blood smears to directly visualize the microorganisms attached to erythrocytes (15).…”

Alteration of integrin-associated protein (CD47) on experimental porcine eperythrozoonosis

“…These parasitic bacteria only have been maintained in splenectomized test animals (Neimark et al 2001). Nonaka et al reported in vitro maintenance system of M. suis in their studies (Nonaka et al 1996).…”