In vitro Mammalian Cell Transformation for Identification of Carcinogens, Cocarcinogens, and Anticarcinogens

Evans, Charles H.; DiPaolo, Joseph A.

doi:10.1007/978-1-4612-5847-6_28

Cited by 2 publications

(1 citation statement)

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“…A CTA of SHE cells measures the conversion of normal mammalian cells to morphologically transformed cells [ 4 ]. The morphologically transformed cells in colonies are characterized by the loss of density-dependent regulation of growth and the formation of colonies with criss-crossed and piled-up cells that are not observed in untreated controls.…”

Section: Introductionmentioning

confidence: 99%

Bhas 42 Cell Transformation Assay for Genotoxic and Non-Genotoxic Carcinogens

Sasaki

Huk

Yamani

et al. 2014

Genotoxicity and DNA Repair

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Cell transformation assays (CTAs) are in vitro carcinogenicity tests measuring morphological transformation of cells either as transformed colonies (SHE cells) or foci (C3H/10T1/2 and BALB/c 3T3 including Bhas 42 cells) derived from a single cell. CTAs such as Bhas 42 CTA can detect both genotoxic and nongenotoxic carcinogens. When used as an initiation assay to test tumor-initiating activity, cells at low density are treated with a test chemical for 3 days, whereas a promotion assay to test for tumor-promoting activity, near-confl uent cells are treated with a test chemical for a period of 10 days. The Bhas 42 CTA has advantages compared with BALB/c 3T3 and other CTAs due to its simplicity, higher sensitivity, less time needed for assay performance, and robustness (exemplifi ed by its adaptation to a high-throughput method). The Bhas 42 CTA has been validated together with other CTAs and recommended for development of an OECD guideline. The assay has already been applied in testing various chemical and physical agents including particles and nanomaterials. Protocols for both 6 and 96-well plate formats of initiation and promotion assays are described in detail.

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Section: Introductionmentioning

confidence: 99%