In vitro manipulation and propagation of medicinal plants

Rout, Gyana Ranjan; Samantaray, Sanghamitra; Das, Purnima

doi:10.1016/s0734-9750(99)00026-9

Cited by 279 publications

(177 citation statements)

References 126 publications

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“…Habitat destruction for agricultural purposes and low seed viability is a major concern for the propagation of these plants. Plant tissue culture techniques are useful for mass multiplication of threatened medicinal plants (Rout et al 2000;Krishnan et al 2011). Consequently, the present investigation is concerned to establish axenic cultures of medicinal plant Artemisia nilagirica var.…”

Section: Introductionmentioning

confidence: 99%

Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

Shinde

Sebastian

Jain

et al. 2016

Physiol Mol Biol Plants

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A reliable protocol has been established for in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood), a valuable medicinal plant from India. A highly proliferating organogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 2.5 lM IAA when nodal explants were cultured on MS medium supplemented with various growth regulators. Further, highest regeneration frequency (83.3 %) of adventitious shoots was observed, when the callus was subcultured on MS medium supplemented with 6-benzylaminopurine (BAP; 2.5 lM) along with 7.5 lM 2-isopentenyl adenine (2-iP). An optimal of 10.16 ± 2.24 shoots were regenerated on medium supplemented with 2.5 lM BAP ? 7.5 lM 2-iP. Quarter strength MS medium supplemented with 10 lM IBA was effective for rooting of the shoots. Ex-vitro plants were normal and were established successfully. Cytological and molecular marker studies showed that regenerated plants showed genetic stability in micro-propagated plants.

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Section: Introductionmentioning

confidence: 99%

Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

Shinde

Sebastian

Jain

et al. 2016

Physiol Mol Biol Plants

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“…The most valuable forms can then be selected and used to establish large-scale culture systems (suspension cell culture or organ culture in bioreactors, or as regenerated plants) for production of those compounds (Ahmad et al 2013;Bourgaud et al 2001;Rout et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Viola uliginosa (Violaceae) for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis

Ślązak

Śliwińska

Saługa

et al. 2014

Plant Cell Tiss Organ Cult

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Viola uliginosa Besser is a European violet having its main distribution range in the Baltic Sea region. Today it is considered endangered and threatened. Species of Violaceae from different genera and sections are known to produce cyclotides, cyclic polypeptides of much interest due to their medicinal properties and chemical structure. The present study introduced a rare species of violet (V. uliginosa) to in vitro culture for biodiversity protection and as a model for cyclotide biosynthesis research in the Violaceae. Leaf and petiole fragments were cultured on MS medium solidified with agar and supplemented with different concentrations of plant growth regulators: TDZ, KIN and 2,4-D. Direct and indirect (via callus) organogenesis was induced on MS supplemented with TDZ (0.5 or 1 mg l -1 ) or with equal concentrations (2 mg l -1 ) of KIN and 2,4-D, followed by callus transfer on 1 mg l -1 TDZ. Shoots were rooted on MS with 2 % sucrose and 0.5 mg l -1 IBA and acclimatized. AFLP marker polymorphism was low but flow cytometry revealed that a large share of the obtained regenerants were tetraploid (2C = 4x = 2.7-2.8 pg), unlike the maternal diploid plants (2C = 2x = 1.4 pg). Eleven different cyclotides were distinguished in the aerial parts of maternal plants. Cyclotide production was significantly higher in tetraploid than in diploid plants regenerated in vitro.

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“…Among the several applications of the tissue culture, we should emphasize that it is a valuable tool to study the metabolism, physiology and development of plants with specific properties, such as, accumulation of active substances of interest ( ROUT et al, 2000;DORAN, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Research with calli formed from fragments of stems, leaves and roots is mainly carried out to determine the culture conditions required by the explants to survive and grow (SAMOYLOV et al, 1998), to study cell development (LITTLE et al, 2000), to exploit products coming from the primary and secondary metabolism and to obtain cell suspension in propagation (ROUT et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

In vitro induction of callus from cotyledon and hypocotyl explants of Glycine wightii (Wight & Arn.) Verdc.

et al. 2003

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-With the objective to promote in vitro callus induction, cotyledon and hypocotyl segments of "perennial soybean" (Glycine wightii (Wight & Arn.) Verdc.) were inoculated in basal medium MS supplemented with sucrose (1.5 e 3%) and 0.8% agar and different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6 -furfurylaminopurine (kinetin). The explants were maintained in a dark growth room at 28 o C. The best callus induction was observed in explants (cotyledon and hypocotyl) maintained in medium containing the combination of 2,4-D (1 mg.L -1 ), kinetin (0.1 mg.L -1 ) and 3% sucrose. To promote callus subculture, the MS

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In vitro manipulation and propagation of medicinal plants

Cited by 279 publications

References 126 publications

Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

Micropropagation of Viola uliginosa (Violaceae) for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis

In vitro induction of callus from cotyledon and hypocotyl explants of Glycine wightii (Wight & Arn.) Verdc.

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