In vitro mass tuberisation as a contribution to potato micropropagation

Rosell, G.; Bertoldi, F. G. De; Tizio, R.

doi:10.1007/bf02357689

Cited by 32 publications

(18 citation statements)

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“…Tuber formation in vitro is supported by a medium supplemented with a high concentration of sucrose and sometimes by the presence of growth regulators (6-benzylaminopurine, 1-naphthylenacetic acid, Rosell et al, 1987) and also by cultivating plantlets in a short photoperiod and in low light intensity. The microplants developed tubers at 10 ~ after two to four months.…”

Section: Resultsmentioning

confidence: 99%

The conservation of potato cultivars

Mix-Wagner¹

1999

Potato Res

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SummaryTo prevent the loss of important genetic information cultivars are conserved in several collections of gene banks. In principal, there are two options for the medium to long-term storage of potato cultivars: storage as in vitro plantlets or microtubers and storage of meristems or shoot-tips in liquid nitrogen. In the Braunschweig potato cultivar collection, 360 cultivars are maintained under slow-growth conditions. Ten microplantlets of each cultivar are stored in test tubes containing filter paper bridges and 5 ml Murashige and Skoog (MS) liquid medium. The cultures were maintained at 10 ~ with a light intensity of 2 klux and 16 hours-day and can be stored under these conditions up to three years. Two hundred and forty-five cultivars are cryostored in liquid nitrogen. About 300 trimmed shoot-tips of each cultivar are incubated in MS-Towill-medium and then transferred into the cryoprotective solution. After an incubation time of about 2 hours trimmed shoot-tips fixed on an aluminium foil were put in cryo vials and stored in a container. The survival rate of the thawed, trimmed shoot-tips varies from 55%-100%. More important for a gene bank, however, is the plant regeneration. The average regeneration of all cultivars is about 40%.

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Section: Resultsmentioning

confidence: 99%

The conservation of potato cultivars

Mix-Wagner¹

1999

Potato Res

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“…1982: Hussey & Stacey, 1984: Rosell et al, 1987: Forti et al, 1991 or minitubers (Struik & Lommen, 1990: van der Zaag, 1990.…”

Section: Propagule Productionunclassified

Innovative propagation methods in seed tuber multiplication programmes

Ranalli

1997

Potato Res

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SummaryThe production of large volumes of vitroplantlets and greenhouse tubers for increasing the rate of multiplication at the start of seed programmes provides the opportunity of reducing the total number of field generations grown before the seed moves into commerce. This implementation is especially useful for countries where high quality potato seed tubers cannot be produced because there are no vector-free production areas.This review covers the lollowing steps: a) laboratory production of microplantlets and microtubers: b) minituber production in the glasshouse: c) storage and dormancy of micro-and minitubers: d) field performance of micro-and minitubers compared with conventiomd seed tubers: e) incorporation of the mentioned propagules in seed production systems.Many optimized protocols are already available for propagating plantlets, inducing microtubers and obtaining minitubers in the glasshouse at all periods of the year. Advanced molecular approaches techniques (RFLP and RAPD) to detect genetic variation in the progeny of these propagules have been described. Investigations carried out in this field have shown genetic stability, with the propagulcs usually reproducing plants true-to-type and tubers without deviants. By contrast, variations were demonstrated in DNA extracted from old suspension cell cultures. Field trials assessed a lower yield potential crops from in vitro propagules compared with conventional seed tubers, mainly due to slow early crop development and the failure of plants caused by early stress after emergence. This may cause problems when the growing season is short because of the necessity for planting late to avoid night frosts and the mandatory haulm killing dates, common in many seed producing areas. Strategies for improving the lield performance of micro-and minitubers are discussed. The most promising crop husbandry techniques appear to be: a) using tubers of a suitable physiological age. properly presprouted and encapsulated: b) optimizing the time application of fertilizer and irrigation, and c) using floating films.Outside the classical seed tuber areas of Northern Europe where the length of the growing period for prc-basic seed is usually not more than 80 days. the growing season is long enough to obtain reasonable yields even from micro-and minitubers.

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“…Similarly, it was observed that in various microtuber production methods, the highest yield resulted from two-phase solid propagating-liquid induction systems [9]. In another study [50], the development of microtubers in liquid and solid media were compared, and it was observed that cultures incubated in a liquid medium resulted in microtubers with more weight than those incubated in a solid medium. In a study conducted by Pelacho et al [51], however, it was found that a half-solid medium resulted in higher tuberization rates and higher tuber weight values than those obtained from liquid media.…”

Section: Figure 3 the Planting Direction Of Single Node Explantsmentioning

confidence: 96%