In Vitro Maturation and Culture of Human Oocytes

Gruhn, Jennifer R.; Kristensen, Stine Gry; Andersen, Claus Yding; Hoffmann, Eva R.

doi:10.1007/978-1-4939-8603-3_3

Cited by 10 publications

(6 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cryopreservation of ovarian cortical tissue followed by autotransplantation has emerged as a promising fertility preservation option (Feigin et al, 2007). Immature oocytes, usually in the germinal vesicle (GV) stage can be found during ovarian tissue handling (Revel et al, 2009;Gruhn et al, 2018) and the ability for maturation in vitro and cryopreservation as mature oocytes, provides an additional option for fertility preservation (Chian et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Age-Dependent in vitro Maturation Efficacy of Human Oocytes – Is There an Optimal Age?

Karavani

Wasserzug-Pash

Mordechai-Daniel

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

In vitro maturation of oocytes from antral follicles seen during tissue harvesting is a fertility preservation technique with potential advantages over ovarian tissue cryopreservation (OTC), as mature frozen and later thawed oocyte used for fertilization poses decreased risk of malignant cells re-seeding, as compared to ovarian tissue implantation. We previously demonstrated that in vitro maturation (IVM) performed following OTC in fertility preservation patients, even in pre-menarche girls, yields a fair amount of oocytes available for IVM and freezing for future use. We conducted a retrospective cohort study, evaluating IVM outcomes in chemotherapy naïve patients referred for fertility preservation by OTC that had oocyte collected from the medium with attempted IVM. A total of 133 chemotherapy naïve patients aged 1–35 years were included in the study. The primary outcome was IVM rate in the different age groups – pre-menarche (1–5 and ≥6 years), post-menarche (menarche-17 years), young adults (18–24 years) and adults (25–29 and 30–35 years). We demonstrate a gradual increase in mean IVM rate in the age groups from 1 to 25 years [4.6% (1–5 years), 23.8% (6 years to menarche), and 28.4% (menarche to 17 years)], with a peak of 38.3% in the 18–24 years group, followed by a decrease in the 25–29 years group (19.3%), down to a very low IVM rate (8.9%) in the 30–35 years group. A significant difference in IVM rates was noted between the age extremes – the very young (1–5 years) and the oldest (30–35 years) groups, as compared with the 18–24-year group (p < 0.001). Importantly, number of oocytes matured, percent of patients with matured oocytes, and overall maturation rate differed significantly (p < 0.001). Our finding of extremely low success rates in those very young (under 6 years) and older (≥30 years) patients suggests that oocytes retrieved during OTC prior to chemotherapy have an optimal window of age that shows higher success rates, suggesting that oocytes may have an inherent tendency toward better maturation in those age groups.

show abstract

Section: Introductionmentioning

confidence: 99%

Age-Dependent in vitro Maturation Efficacy of Human Oocytes – Is There an Optimal Age?

Karavani

Wasserzug-Pash

Mordechai-Daniel

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Although intensive research has been conducted, the viability of viable embryos produced in vitro is still far less than that produced in vivo. Currently, a common problem with embryos produced in vitro is that the oocyte cytoplasm is not fully formed (Gruhn et al., 2018 ). Studies have shown that as follicles grow, oocytes gradually gain meiotic and embryonic developmental abilities; however, oocytes obtained from the same size follicles are heterogeneous, which may explain the difference in the chromatin configurations for oocyte meiosis, cytoplasmic maturation ability and embryo development ability (Marchal et al., 2002 ).…”

Section: Discussionmentioning

confidence: 99%

Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes

Yao

Cheng

Liu

et al. 2021

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Background: Germinal vesicle (GV) chromatin configurations of oocytes are proposed to be related to oocyte competence and may reflect the quality of oocyte. Currently, a limited number of published studies investigated the GV chromatin configurations of guinea pig oocytes.Objective: In this study on the in vitro maturation (IVM) of guinea pig oocytes, we examined the changes in their GV chromatin configurations during meiotic progression.Methods: Based on the degree of chromatin compaction, the GV chromatin configurations of guinea pig oocytes could be divided into three categories depending on whether the nucleolus-like body (NLB) was surrounded or partly surrounded by compacted chromatin, namely the uncondensed (NSN), the intermediate type (SN-1) and the compacted type (SN-2). Results:The percentage of cells displaying the SN-2 configuration increased with the growth of guinea pig oocytes, suggesting that this configuration presents the potential for maturation in oocytes. Oocytes derived from larger follicle exhibited increased meiotic potential. Serum starvation affected the GV chromatin configurations of guinea pig oocytes. Conclusions:Collectively, these results suggest that the SN-2 type might be a more mature form of configuration in guinea pig oocyte, whose proportion was associated with the follicle size and susceptible to the environment (e.g. serum concentration).

show abstract

“…Culture systems have been developed to support isolated multilaminar (preantral) growing follicles isolated from the human ovarian cortex ( 564 – 568 ) or developed in vitro from primordial stages ( 311 , 376 , 543 , 544 ). Human ovarian follicles can grow up to several millimeters in diameter therefore, maintaining their structure in vitro is challenging and tissue-engineering principles have been applied to tackle this problem ( 540 , 569 ).…”

Section: In Vitro Development Of Oocytesmentioning

confidence: 99%

Making a good egg: human oocyte health, aging, and in vitro development

Telfer

Grosbois

Odey

et al. 2023

Physiological Reviews

View full text Add to dashboard Cite

Mammalian eggs (oocytes) are formed during fetal life and establish associations with somatic cells to form primordial follicles that create a store of germ cells (the primordial pool). The size of this pool is influenced by key events during the formation of germ cells and by factors that influence the subsequent activation of follicle growth. These regulatory pathways must ensure that the reserve of oocytes within primordial follicles in humans lasts for up to 50 years, yet only approximately 0.1% will ever be ovulated with the rest undergoing degeneration. This review outlines the mechanisms and regulatory pathways that govern the processes of oocyte and follicle formation and later growth, within the ovarian stroma, through to ovulation with particular reference to human oocytes/follicles. In addition, the effects of aging on female reproductive capacity through changes in oocyte number and quality are emphasized, with both the cellular mechanisms and clinical implications discussed. Finally, details of current developments in culture systems that support all stages of follicle growth to generate mature oocytes in vitro, and emerging prospects for making new oocytes from stem cells are outlined.

show abstract

In Vitro Maturation and Culture of Human Oocytes

Cited by 10 publications

References 7 publications

Age-Dependent in vitro Maturation Efficacy of Human Oocytes – Is There an Optimal Age?

Age-Dependent in vitro Maturation Efficacy of Human Oocytes – Is There an Optimal Age?

Relationship between chromatin configuration and in vitro maturation ability in guinea pig oocytes

Making a good egg: human oocyte health, aging, and in vitro development

Contact Info

Product

Resources

About