In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa

Khandoker, M. A. M. Y.; Reza, Mansur; Asad, L.Y.; Saha, Sourav Mohan; Apu, A. S.; Hoque, S. A. Masudul

doi:10.3329/bjas.v41i1.11969

Cited by 2 publications

(3 citation statements)

References 20 publications

(12 reference statements)

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Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

“…Low genetic variability is one of the main threats to the long-term maintenance of the species. Assisted reproductive technology such as production of in vitro embryos using oocytes of domestic cattle and wisent semen will not only help to maintain the existing genetic resources of the species, but also to involve this species in basic research on the physiology of development and use in creations of new bred forms [2][3][4].Cow oocytes are a universal object for interspecific hybridization. In publications, there is evidence of their in vitro fertilization using semen of gaur [5], antelope [6], horse [7], sheep [8], and donkey [9].…”

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confidence: 99%

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IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

Сингина¹,

Багиров²,

Danch³

et al. 2016

S-h. biol.

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A b s t r a c t European bison or wisent (Bison bonasus)is a rare species that is endangered. The technology for producing in vitro embryos using oocytes of cows and wisent semen can be an effective tool for conservation of genetic resources of this species and their rational use in basic research on the physiology of development and to create the new animals' types. However, the methods for production of in vitro embryos using the above-mentioned germ cells are currently not described in the literature. In this work, we first attempted to obtain the hybrid cow-wisent embryos in vitro using the IVM/IVF/IVC protocol, developed for domestic cattle (Bos taurus). Frozen epididymal sperm derived from wisent was thawed and prepared by the swim-up procedure. Matured oocytes were coincubated during 18 h together with prepared sperm in Fert-Talp medium, which was replaced then by embrional medium where the embryos were cultured until the blastocyst state. Additionally, for positive control, a part of matured bovine oocytes were fertilized with frozen/thawed ejaculated bull sperm. Fertilizing capacity of male cells of both species was assessed according to the nature of spermatozoa-egg interactions, as well as the capacity of fertilized bovine oocytes to subsequent embryonic development. It was found that the rate of oocytes with sing polyspermy was higher for fertilization with wisent sperm comparing to allogenic insemination (21.6 vs. 8.5 %, P < 0.05). There were no differences between two types of fertilizations nether for the sperm penetration rate (90.0±0.3 vs. 93.3±1.7 % for bull and wisent, respectively), nor for the rate of normal fertilization (78.3±1.7 % vs. 73.2±2.3 %). The similar cleavage and blastocyst formation rates were observed for two fertilization types. Our data indicate the similarity in the mechanisms of oocyte activation and embryonic development in cow and wisent females. In addition, it is obvious that IVM/IVF/IVP protocol, in general, allows in vitro producing cattle-wisent hybrid embryos, however, high levels of polyspermy observed in heterogeneous fertilization, indicates the necessity to adjust the method.Keywords: cattle oocytes, the European bison sperm, fertilization in vitro, hybrid embryos European bison, or wisent, (Bison bonasus) is a rare endangered species. Current European bison population is strongly inbred. All modern wisents come from 12 individuals kept in the zoos and reserves in the early XX century [1]. Low genetic variability is one of the main threats to the long-term maintenance of the species. Assisted reproductive technology such as production of in vitro embryos using oocytes of domestic cattle and wisent semen will not only help to maintain the existing genetic resources of the species, but also to involve this species in basic research on the physiology of development and use in creations of new bred forms [2][3][4].Cow oocytes are a universal object for interspecific hybridization. In publications, there is evidence of their in vitro fertilization using semen of gaur...

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Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

mentioning

confidence: 99%

IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

Сингина¹,

Багиров²,

Danch³

et al. 2016

S-h. biol.

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“…Research on buffalo IVEP is remained at very preliminary stages including evaluation of follicular statistics, COCs recovery rate, grading of COCs and in vitro maturation of COCs (Khandoker et al, 2011 and2012). So, adoption of culture system for development of embryos up to blastocyst stage is essential.…”

Section: Introductionmentioning

confidence: 99%

In vitro production of zygote from slaughterhouse driven buffalo oocyte

Deb

Miraz

Hossain

et al. 2020

Bangladesh J. of Livestock Res.

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Buffalo is a highly potential animal species in terms of milk and meat production but traditionally they are regarded as poor breeder. In vitro embryos production technology has been introduced in many countries to improve reproductive efficiency of buffalo. Considering the above fact, the present study was undertaken aiming to produce in vitro buffalo embryo in the laboratory. Ovaries of slaughtered buffaloes were collected from abattoir and transported to the laboratory within 4 to 5 hr of slaughter. Cumulusoocyte- complexes (COCs) possessing an even cytoplasm and covered with minimum 3 layers of compact cumulus cells was selected for in vitro maturation (IVM) for 24 hr (5% CO2 in air at 38.5°C with maximum humidity). After IVM, the presumptive matured COCs were co-cultured with capacitated fresh spermatozoa for 18 hr After IVF, the presumptive zygote were denuded, washed and transferred in to in vitro culture medium (IVC 1) for 3 days. After three days cleavage were recorded and 4 cell embryos were transferred in to in vitro culture media II for next 2 days. The development of embryos was evaluated on day 6. A total of 227 buffalo ovaries were collected from the slaughterhouse and categorized into 2 groups based on presence (n=83) or absence (n=144) of corpus luteum (CL). A total of 1464 follicles were counted on the ovarian surface, 1066 being from CL absent and 398 from CL-containing ovaries. A significantly higher (P<0.01) number of follicles, aspirated follicles, normal COCs and total COCs (7.4 ± 0.21, 5 ± 0.00, 1.98 ± 0.77 and 2.98 ± 0.16 respectively) were observed in CL-absent ovaries than those aspirated from CL-containing ovaries (4.80 ± 0.17, 3.92 ± 0.95, 0.88 ± 0.60 and 1.88 ± 0.16 respectively). Total 358 normal COCs were set for in vitro maturation and underwent for IVF and IVC. Results showed that cleavage rates were 56.42%. Among the cleaved embryos, 137 were at 2-cell stage and 65 were at 4-cell stage. Therefore, development rate to 2 cell and 4-cell stage was 38.27% and 18.15% respectively. No embryo developed beyond 4-cell stage. This result indicates that follicle and oocyte numbers and oocyte quality are associated with CL of ovaries and current culture system support in vitro embryo production upto 4-cell stage. The in vitro culture condition may be improved for increasing efficiency of embryo production. Bangladesh J. of Livestock Res. 21-25: 127-132, 2018

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In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa

Cited by 2 publications

References 20 publications

IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

In vitro production of zygote from slaughterhouse driven buffalo oocyte

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