In vitro maturation of cumulus–partially enclosed immature human oocytes by priming with gonadotropin

Yan, Jie; Yu, Yang; Yan, Liying; Liu, Zichuan; Liu, Ping; Feng, Hua; Zhou, Qi; Qiao, Jie

doi:10.1016/j.fertnstert.2011.06.054

Cited by 17 publications

(19 citation statements)

References 33 publications

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“…On the other hand, 0PN embryos produced by abnormal fertilization (1PN, >2PN) or via parthenogenetic activation may produce low IRs. Cleavage embryos from abnormal fertilization (1PN, >2PN) (12) and parthenogenetic activation (19) yield low blastulation rates, so blastocyst culture may be an efficient method for exclusion of embryos derived from abnormal fertilization (1PN, >2PN) or parthenogenetic activation. In this study, the IR of the 0PN cleavage frozen was lower than that of the 2PN cleavage frozen-C, while the IR was comparable between the 0PN blast frozen and the 2PN blast frozen-C groups.…”

Section: Discussionmentioning

confidence: 99%

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Value of transferring embryos that show no evidence of fertilization at the time of fertilization assessment

Lin

Yuan

et al. 2015

Fertility and Sterility

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Transfer of 0PN embryos from fresh or frozen-thawed cycles results in pregnancies and live births. Nonpronuclear embryos have a lower IR than 2PN embryos, but if the embryos are cultured to the blastocyst stage and then are frozen, their IRs approach that of 2PN embryos in subsequent frozen-thawed cycles. The culture of 0PN embryos to the blastocyst stage may select for embryos with a near-normal IR.

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Section: Discussionmentioning

confidence: 99%

“…* D uring in vitro fertilizationembryo transfer (IVF-ET) treatment for infertility, assessment of fertilization is generally performed [16][17][18][19] hours after insemination, with the presence of two pronuclei (2PN) indicating normal fertilization.…”

mentioning

confidence: 99%

Value of transferring embryos that show no evidence of fertilization at the time of fertilization assessment

Lin

Yuan

et al. 2015

Fertility and Sterility

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“…Such errors could influence the outcome of oocytes. In an experimental study of Yan et al [9], adding exogenous gonadotropins to in vitro germinal vesicle and MI oocyte did not induce better maturation; furthermore, gonadotropins at the current concentration did not increase spindle or chromosomal abnormalities in MII oocytes maturated from either GV-or MI-stage oocytes in vitro. Other authors reported similar treatment errors with live birth outcome [10].…”

Section: Discussionmentioning

confidence: 76%

Self-oocyte activation and parthenogenesis: an unusual outcome of a misconducted IVF cycle

Socolov

Ebner²,

Gorduza

et al. 2015

Gynecological Endocrinology

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A rare cause of infertility is the lack of fertilisation with the spontaneous activation of oocytes, leading to parthenogenesis. We present such a case. The patient was a G1P0 38-year-old woman of African ethnicity, who requested an in vitro fertilisation (IVF) with donor sperm. She received a stimulation protocol of 75 IU of FSH/LH from day 3 of the cycle, which she interrupted after 2 d, and restarted with the same dosage for another 3 d from day 7, plus one administration of GnRH antagonist in day 10 of the cycle. With a follicle reaching 19 mm on day 11, estradiol of 325 ng/ml, ovulation was induced with hMG 5000 UI, and oocyte pick-up performed at 30 h. One oocyte was retrieved, and good-quality sperms were added to the insemination procedure. No fecundation occurred at 20 h, with the extruded oocyte separated from the granulosa wall. At 40 h and 64 h the aspect was of three cells, one cell with one nucleus, the others with high granulation and no visible nuclei. This case shows an unusual self-activation oocyte in a poorly managed IVF cycle. The patient will be further evaluated, to decide if a better managed stimulation protocol would prevent recurrence.

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“…The method of immunofluorescence manipulation was referenced in our previous study [21]. The mouse IVM oocytes were first rinsed and fixed in 4% paraformaldehyde for 20 min, permeabilized with 0.2% Triton X-100 for 30 min, and blocked in 3% BSA in PBS for 2 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection

Zhao

et al. 2014

PLoS ONE

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Implantation failure and early pregnancy loss have been reported to be closely related to the quality of mammalian oocytes; however, the pregnant outcome of embryos from in-vitro matured (IVM) oocytes remains unknown. In this study we examined spindle assembly and chromosome segregation during differentiation, and the duration of IVM of mouse oocytes. The resulting implantation and pregnancy outcomes were analyzed to clarify the relationship between the spindle and chromosomes of IVM oocytes and implantation and early pregnancy. Cumulus-enclosed germinal vesicle oocytes were collected and randomly cultured in IVM medium with different IVM durations. One part of IVM oocytes were analyzed the spindle and chromosome morphology by immunofluorescence method, and the other part of them were fertilized by intracytoplasmic sperm injection. The resulting embryos were transferred into pseudo-pregnant female mice, and the post-implantation and full term development was observed. The chromosome aberrations and incorrect spindle assembly seems not affect the early development and blastocyst cell number derived from IVM oocytes, however the development potential of the resulting embryos after implantation were significant decreased with the ratio increasing of chromosome aberrations and incorrect spindle assembly. Accordingly, the full-term development was also decreased. In conclusion, the present study showed the spindle assembly of in vitro-matured oocytes was one of the most important factors that affected the implantation and ongoing pregnancy rates of IVM oocytes, and the improvement by an appropriate duration of maturation in vitro will enhance the post-implantation development potential of the resulting embryos, and decrease implantation failure and early pregnancy loss.

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In vitro maturation of cumulus–partially enclosed immature human oocytes by priming with gonadotropin

Cited by 17 publications

References 33 publications

Value of transferring embryos that show no evidence of fertilization at the time of fertilization assessment

Value of transferring embryos that show no evidence of fertilization at the time of fertilization assessment

Self-oocyte activation and parthenogenesis: an unusual outcome of a misconducted IVF cycle

Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection

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