In vitro maturation of equine oocytes: Effect of follicular size, cyclic stage and season

Brück, Ingrid; Grøndahl, C.; Høst, Thomas; Greve, T.

doi:10.1016/0093-691x(96)00143-4

Cited by 36 publications

(24 citation statements)

References 12 publications

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“…(P<0.01) in hot versus cool seasons (Hamam et al, 2001). On the contrary, Brück et al (1996) in equine and Rivera et al (2000) in bovine, did not find any effect of season on in vitro production of embryos in temperate or subtropical environments. The variation in response to the season could be attributed to the individual capability of different species or breeds for heat-tolerance.…”

Section: Oocyte Meiotic Chromosomesmentioning

confidence: 89%

Hot climate effects and their amelioration on some productive and reproductive traits in rabbit does.

Yassein¹,

Gh²,

Maghraby³

et al. 2010

World rabbit sci.

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This study aimed to improve productive and reproductive performance of female rabbit does during the summer season "hot climate" using vitamin C or cooled water in combination with equine chorionic gonadotropin (eCG) treatment. Sixty New Zealand White rabbit does were assigned to three groups, according to drinking-water treatment: 1) fresh tap water without any supplementation (control, C), 2) cooled drinking water (10-15ºC) (CW), and 3) fresh tap water supplemented daily with added ascorbic acid (1 g/L) (vitamin C). Twenty four hours before mating, does of each group were randomly divided into two subgroups; H does were intravenously injected with 40 IU/doe eCG, while NH does did not receive any hormonal treatment. Productive and reproductive performance were signifi cantly (P<0.05) improved in the treated groups. Kit weights at kindling and weaning were greater (P<0.05) in both vitamin C and cooled water groups than in the control regardless of hormonal treatment. It is worthy noticed that conception rate and litter size at birth were adversely affected by eCG, especially in does drinking vitamin C. The percentage of mature oocytes was lower (P<0.01) in control than in treated groups. The oocyte maturation rate improved after treatment with vitamin C to reach 80% compared to 66% in control group. In conclusion, cool drinking water or vitamin C is recommended for alleviating heat stress during summer in rabbits.

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Section: Oocyte Meiotic Chromosomesmentioning

confidence: 89%

Hot climate effects and their amelioration on some productive and reproductive traits in rabbit does.

Yassein¹,

Gh²,

Maghraby³

et al. 2010

World rabbit sci.

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“…Results are presented as percentage of oocytes for each category of meiotic stage. According to the characterization of nuclear stages of equine oocytes used in the literature [after Hoechst staining: Hinrichs et al, 1993aHinrichs et al, , b, 1995Alm and Hinrichs, 1996; after orcein staining: Zhang et al, 1989;Willis et al, 1991;Alm and Torner, 1994, Del Campo et al, 1995, Torner and Alm, 1995Brück et al, 1996Brück et al, , 1997, oocytes diagnosed as Dia after Hoechst were assumed to correspond to those in the stage of GVBD after orcein staining. ¯2 analysis was used to analyze differences in the distribution of nuclear stages.…”

Section: Discussionmentioning

confidence: 99%

“…The time of transport has been reported between 2.5 and 6 h [Alm and Torner, 1994;Willis et al, 1994;Brück et al, 1996] and the time interval between collection and fixation is generally longer for oocytes after slaughter than after collection by in vivo follicular aspiration. It is known that equine COCs removed from follicles resume meiosis after 8 and 16 h of culture [Hinrichs et al, 1993a], and it has been suggested that resumption of meiosis is prevented by a soluble mediator which is transmitted via gap junctions between mural granulosa cells, cumulus cells and oocyte [Hinrichs et al, 1995;Yding Andersen et al, 1999].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the incidence of germinal vesicle (GV) stages has been reported to be between 3 and 80%; the incidence of resumption of meiosis, i.e. germinal vesicle breakdown (GVBD) up to metaphase I (MI) stages between 3 and 55% and the incidence of metaphase II (MII) stages between 0 and 13% [Desjardins et al, 1985;Okolski et al, 1991;Willis et al, 1991Willis et al, , 1994Bézard and Palmer, 1992;Hinrichs et al, 1993aHinrichs et al, , 1995Alm and Torner, 1994;Del Campo et al, 1995;Torner and Alm, 1995;Brück et al, 1996Brück et al, , 1997.…”

Section: Introductionmentioning

confidence: 99%

“…Only few morphological descriptions of the different chromatin configurations of equine oocytes are available. For the determination of the nuclear stages of oocytes by light and fluorescence microscopy, the DNA can be visualized by fluorescent staining with Hoechst [Hinrichs et al, 1993b;Goudet et al, 1997Goudet et al, , 1998], by staining with orcein after fixation with acetic alcohol [Fulka and Okolski, 1981;Okolski et al, 1991;Bézard and Palmer, 1992;Alm and Torner, 1994;Del Campo et al, 1995;Torner and Alm, 1995;Brück et al, 1996Brück et al, , 1997 or by staining with lacmoid after fixation in glutaraldehyde followed by acetic alcohol [Zhang et al, 1989;Willis et al, 1991Willis et al, , 1994. During fluorescence microscopy after Hoechst staining, only the chromatin is visible, while after orcein or lacmoid staining additional cellular structures in the nucleoplasm and cytoplasm can be taken into account for evaluation.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Evaluation of Nuclear Morphology of Equine Oocytes Aspirated in vivo and Stained with Hoechst and Orcein

Bézard

Bøgh

Duchamp

et al. 2002

Cells Tissues Organs

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Nuclear maturation of equine oocytes was assessed immediately after in vivo collection. A double-staining technique (Hoechst and orcein) was used on the same oocytes to visualize nuclear morphology, i.e. to evaluate the chromatin configurations of each oocyte after Hoechst in relation to the nuclear morphology after orcein staining. The proportion of oocytes evaluated as germinal vesicle stages was significantly (p < 0.02) lower after Hoechst (14.5%) than after orcein staining (29.0%), while the incidence of the so-called dense chromatin stage was assessed to be higher (p < 0.05) after Hoechst than after orcein staining (14.5 vs. 6.5%). There was no difference between Hoechst and orcein staining in the incidence of diakinesis and germinal vesicle breakdown stages, respectively (44.9 vs. 42.0%), and the same applied for metaphase I (11.6 vs. 8.0%), metaphase II (7.2 vs. 8.0%) and degenerated stages (7.2 vs. 6.5%). It was concluded that the interpretation of the meiotic stages may differ between Hoechst and orcein staining and in a large proportion of equine oocytes the nuclear border may not be visualized on orcein staining.

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Embryo Transfer andin vitroFertilization

Hasler¹

2007

Comparative Reproductive Biology

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In vitro maturation of equine oocytes: Effect of follicular size, cyclic stage and season

Cited by 36 publications

References 12 publications

Hot climate effects and their amelioration on some productive and reproductive traits in rabbit does.

Hot climate effects and their amelioration on some productive and reproductive traits in rabbit does.

Comparative Evaluation of Nuclear Morphology of Equine Oocytes Aspirated in vivo and Stained with Hoechst and Orcein

Embryo Transfer andin vitroFertilization

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