In-vitro maturation of germinal vesicle and metaphase I eggs prior to cryopreservation optimizes reproductive potential in patients undergoing fertility preservation

Lee, Joseph A.; Sekhon, L.; Grunfeld, L.; Copperman, A.B.

doi:10.1097/gco.0000000000000062

Cited by 16 publications

(11 citation statements)

References 39 publications

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“…Although nowadays comparable high survival rates (80-85%) have been reported for oocytes vitrified prior to or post-IVM [43,44], differences found in terms of cellular alterations and clinical outcomes claim for an impairment in the ability of human IVM oocytes to mature, fertilize, and cleave, that seems to be dependent on the stage in which vitrification occurs.…”

Section: Oocyte In Vitro Maturation Prior To and Post-vitrificationmentioning

confidence: 85%

“…However, even if oocyte morphology and zona pellucida birefringence were not altered [51,52], the maturation potential was reduced by Cryotop vitrification of human immature GV-stage oocytes, as seen by reduced maturation rate and oocyte viability respect to the fresh counterpart [36,53]. Superior maturation rates of GV and MI oocytes vitrified post-IVM (≈ 64%), have been demonstrated in terms of mitochondria, chromatin, and spindle formation, respect to those vitrified prior to IVM (≈ 33%) [44]. Moreover, no detrimental effects were seen on the meiotic spindle configuration between oocytes matured in vitro and those vitrified after IVM [32].…”

Section: Oocyte In Vitro Maturation Prior To and Post-vitrificationmentioning

confidence: 99%

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Vitrification of human immature oocytes before and after in vitro maturation: a review

Khalili

Shahedi

Ashourzadeh

et al. 2017

J Assist Reprod Genet

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The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation may offer some advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation and postponing childbirth. It also eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing. In addition, a successful oocyte cryopreservation program could eliminate the need for donor and recipient menstrual cycle synchronization. Recent advances in vitrification technology have markedly improved the oocyte survival rate after warming, with fertilization and implantation rates comparable with those of fresh oocytes. Healthy live births can be achieved from the combination of IVM and vitrification, even if vitrification of in vivo matured oocytes is still more effective. Recently, attention is given to highlight whether vitrification procedures are more successful when performed before or after IVM, on immature GV-stage oocytes, or on in vitro matured MII-stage oocytes. In this review, we emphasize that, even if there are no differences in survival rates between oocytes vitrified prior to or post-IVM, reduced maturation rates of immature oocytes vitrified prior to IVM can be, at least in part, explained by underlying ultrastructural and biomolecular alterations.

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Section: Oocyte In Vitro Maturation Prior To and Post-vitrificationmentioning

confidence: 85%

Section: Oocyte In Vitro Maturation Prior To and Post-vitrificationmentioning

confidence: 99%

Vitrification of human immature oocytes before and after in vitro maturation: a review

Khalili

Shahedi

Ashourzadeh

et al. 2017

J Assist Reprod Genet

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show abstract

“…The maturation rate following thawing and IVM after vitrification at the GV stage is 50.8%, significantly lower than the rate of 70.4% for the IVM of fresh material . Several reports have yielded consistent results; therefore, it is common to cryopreserve matured oocytes when using the vitrification method.…”

Section: Cryopreservation Of Oocytes For Fertility Preservationmentioning

confidence: 97%

In vitro maturation of human immature oocytes for fertility preservation and research material

Shirasawa

Terada

2017

Reprod Medicine & Biology

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AimIn recent years, the importance of fertility preservation (FP) has increased. In vitro maturation (IVM), an important technique in FP, has started to be used in the clinic, but controversies persist regarding this technique. Here, a survey of IVM for FP is provided.MethodsBased on a literature review, the applications of FP, methods of FP, IVM of oocytes that had been collected in vivo and ex vivo, maturation of oocytes after IVM for FP, cryopreservation of oocytes for FP, explanation of the procedures to patients, and recent research on FP using IVM were investigated.ResultsAlthough IVM for FP remains controversial, the application of FP is expected to expand. Depending on the age and disease status of the patient, various methods of oocyte collection and ovarian stimulation, as well as various needle types and aspiration pressures, have been reported. The maturation rate of IVM in FP ranges widely and requires optimization in the future. In regard to cryopreservation for matured oocytes, the vitrification method is currently recommended.ConclusionRegarding FP for patients with cancer, the treatment of cancer is prioritized; thus, the time and use of medicines are often constrained. As several key points regarding IVM remain unclear, well‐designed and specific counseling for patients is necessary.

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“…A number of groups have reported effects of cryopreservation of GV stage oocytes. The effects of slow freezing are controversial: two studies showed that slow freezing at GV stage had better outcome than at post-IVM MII stage [ 16 , 17 ], while several other studies reported low maturation rates when slow freezing at the GV stage was performed [ 18 , 19 , 33 ]. Vitrification at GV stage was reported to negatively affect maturation rates [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Production of Live Offspring from Vitrified-Warmed Oocytes Collected at Metaphase I Stage

Chang¹,

Wu²,

Xu³

et al. 2016

PLoS ONE

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Vitrification of matured oocytes is widely adopted in human clinics and animal research laboratories. Cryopreservation of immature oocytes, particularly those at metaphase I (MI), remains a challenge. In the present work, mouse MI oocytes denuded of cumulus cells were vitrified and warmed (V/W) either prior to (V/W-BEFORE-IVM, n = 562) or after (V/W-AFTER-IVM, n = 664) in vitro maturation (IVM). Derivative metaphase II (MII) oocytes were then used for intracytoplasmic sperm injection (ICSI). In the control groups, in vivo matured MII oocytes were used freshly (FRESH-MII, n = 517) or after V/W (MII-V/W, n = 617). In vitro and in vivo developmental competencies were compared among groups. Satisfactory blastocyst rates were achieved in V/W-BEFORE-IVM (27.5%) and V/W-AFTER-IVM (32.4%) groups, albeit as expected still lower than those from fresh-MII (56.1%) or MII-V/W (45.6%) oocytes. Similarly, the term development rates from V/W-BEFORE-IVM and V/W-AFTER-IVM were 12.4% and 16.7% respectively, acceptable but lower than those of the fresh-MII (41.2%) and MII-V/W (23.3%) groups. These data demonstrate that oocytes collected at MI stage are amenable to V/W, which can be performed before or after IVM with acceptable development rates including production of healthy pups. These findings provide useful knowledge to researchers and clinical practitioners for preservation and use of the otherwise discarded MI oocytes.

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In-vitro maturation of germinal vesicle and metaphase I eggs prior to cryopreservation optimizes reproductive potential in patients undergoing fertility preservation

Cited by 16 publications

References 39 publications

Vitrification of human immature oocytes before and after in vitro maturation: a review

Vitrification of human immature oocytes before and after in vitro maturation: a review

In vitro maturation of human immature oocytes for fertility preservation and research material

Production of Live Offspring from Vitrified-Warmed Oocytes Collected at Metaphase I Stage

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