In Vitro Maturation of Maize Pollen

Pareddy, D. R.; Petolino, J. F.

doi:10.1007/978-1-4612-2958-2_48

Cited by 4 publications

(6 citation statements)

References 9 publications

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“…The maturation rate of microspores in our study was 19.3% in optimal medium, an improvement over wheat (1%: Stauffer et al 1991) and maize (14%: Pareddy and Petolino 1992). Pollen development in vitro was similar to in vivo development in this study.…”

Section: Discussionsupporting

confidence: 83%

“…To be best of our knowledge, successful in vitro maturation of microspores has been reported only in a few species: wheat (Stauffer et al 1991), maize (Pareddy and Petolino 1992), snapdragon (Barinova et al 2002), and tobacco (Aziz and Machray 2003). The maturation rate of microspores in our study was 19.3% in optimal medium, an improvement over wheat (1%: Stauffer et al 1991) and maize (14%: Pareddy and Petolino 1992).…”

Section: Discussionmentioning

confidence: 98%

“…This altered developmental pathway has been the focus of researchers hoping to derive haploid plants of many important crops species (Hu 1996). In contrast, research of in vitro maturation of microspores or immature pollen into functional pollen grains has received relatively little attention since it was reported in lily (Tanaka et al 1980), tobacco (Kyo and Harada 1986;Benito et al 1988;Tupý et al 1991;Touraev and Heberle-Bors 1999), wheat (Stauffer et al 1991), maize (Pareddy and Petolino 1992) and Antirrhinum majus (Barinova et al 2002). However, such an in vitro maturation system can provide possibilities for direct observation of the various events, including morphology, biophysiology and biochemistry, occurring throughout the microspore developmental process and for understanding their molecular regulation.…”

Section: Introductionmentioning

confidence: 87%

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In vitro maturation and germination of Orychophragmus violaceus microspores

Zhao

Wang

et al. 2007

Plant Cell Tiss Organ Cult

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Microspores cultured in vitro can be regarded as a system to study gene regulation, cell fate determination and cell differentiation during pollen development as well as an alternative method of genetic transformation in plants. In our study, pollen development and viability in Orychophragmus violaceus in vivo were determined and then pollen from the late unicellular stage was cultured in vitro. MS liquid medium + White vitamins + 2% (V/V) coconut milk + 0.5 M maltose, pH = 7.0 was the most appropriate for in vitro culture of Orychophragmus violaceus microspores. With this medium, the rates of in maturation and germination were 19.3% and 4.7%, respectively. Liquid medium with 0.6 M maltose + 1.6 mM boric acid + 2.9 mM Ca(NO 3 ) 2 + 29.6 lM vitamin B1, pH = 7.0 was optimal for germination of pollen matured in vivo. The rate of germination was 70.7%. Pollen matured in vitro cultured in similar medium exhibited a rate of germination of 62.7%. Hence, the experimental study showed that in vitro maturation of microspores is feasible and this experimental system can be applied to further theoretical and practical research.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 87%

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In vitro maturation and germination of Orychophragmus violaceus microspores

Zhao

Wang

et al. 2007

Plant Cell Tiss Organ Cult

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“…In maize, only spikelet culture yields a high percentage (50 to 70%) of normal, germinable pollen. When isolated maize microspores were cultured, only 5 to 20% of them developed into starch-filled trinucleate grains, and fewer than 1% of these in vitro-matured grains germinated (Pareddy and Petolino, 1992). In vitro maturation does not completely mimic the in vivo situation.…”

Section: Genes Controlling Cell Fate During Microsporogenesismentioning

confidence: 99%

“…Severa1 groups have reported success in culturing immature microspores and achieving pollen maturation in vitro, although culture of more immature stages often requires anther culture (Pareddy and Petolino, 1992;Stapleton and Bedinger, 1992) rather than free microspore culture. The frequency of functional (as assessed by pollen germination) pollen obtained from in vitro maturation varied from plant to plant and depended on the stage of isolation.…”

Section: Genes Controlling Cell Fate During Microsporogenesismentioning

confidence: 99%

Male Gametophyte Development.

McCormick

1993

Plant Cell

423

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Endogenous polyamine content during in vivo maturation and in vitro culture of maize pollen

1995

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Changes in polyamine content during in vivo maturation and in vitro culture of maize (Zea mays L.) pollen were studied. The endogenous content of free, conjugated and bound polyamines was analyzed during 30 days of pollen evolution, in both developmental pathways (microsporogenesis and androgenesis). The induction of androgenesis from cold-pretreated uninucleate pollen results, in most of cases, in a lower total polyamine content than that of the in vivo uninucleate pollen. These differences indicate that polyamine metabolism is altered during the induction of androgenesis, and this could be a consequence of increased polyamine assimilation. In general, pollen stages that involve cell division (tetrades, pre-anthesis pollen and four-day cultured pollen) are characterized by a predominance of free Spd. The increase of Spd and Spm in 15-day cultured pollen, when the first embryoids are formed, outline the possible implication of these polyamines in embryogenetic processes. Furthermore, these findings may contribute to the improvement of maize androgenesis yield, especially in recalcitrant genotypes, by the exogenous application of polyamines or polyamine-inhibitors to the culture medium.Abbreviations: PAS = polyamines; Put = putrescine; Spd = spermidine; Spm = spermine; S = free polyamine fraction; SH = conjugated polyamine fraction; PH = bound polyamine fraction.

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In Vitro Maturation of Maize Pollen

Cited by 4 publications

References 9 publications

In vitro maturation and germination of Orychophragmus violaceus microspores

In vitro maturation and germination of Orychophragmus violaceus microspores

Male Gametophyte Development.

Endogenous polyamine content during in vivo maturation and in vitro culture of maize pollen

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