In vitro maturation or in vitro fertilization for women with polycystic ovaries? A case–control study of 194 treatment cycles

Grémeau, Anne-Sophie; Andreadis, Natasha; Fatum, Muhammad; Craig, Jo; Turner, Kevin B.; McVeigh, Enda; Child, Tim

doi:10.1016/j.fertnstert.2012.04.046

Cited by 136 publications

(82 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Higher zinc levels in cumulus cells matured in vitro may maintain higher pSMAD2 signaling in cumulus cells. Oocytes matured in vitro are less fertile than those matured in vivo, at least for women with polycystic ovarian syndrome (Child et al 2002, Gremeau et al 2012. It could be that altered zinc homeostasis in oocytes or cumulus cells in vitro disrupts oocyte maturation and/or cumulus cell function.…”

Section: Discussionmentioning

confidence: 99%

“…Dramatic effects of zinc deficiency have been uncovered in oocytes (Kim et al 2010, Suzuki et al 2010b) and cumulus cells (Tian & Diaz 2012) during the periovulatory transition. Currently, the success of human IVM is highly variable (4-35% clinical pregnancy/ transfer) (Cha et al 1991, Trounson et al 1994, Child et al 2001, Gremeau et al 2012, Guzman et al 2012. The ability to reliably mature oocytes in vitro would reduce reliance on human chorionic gonadotropin (hCG) injections which are associated with ovarian hyperstimulation syndrome (Aboulghar & Mansour 2003).…”

Section: Zinc Homoeostasis In the Oocytementioning

confidence: 99%

See 1 more Smart Citation

Oocyte–cumulus cell interactions regulate free intracellular zinc in mouse oocytes

Lisle¹,

Anthony²,

Randall³

et al. 2013

Reproduction

View full text Add to dashboard Cite

Zinc increases in the oocyte during maturation and is required for progression and completion of meiosis. The objective of this study was to determine whether cumulus cells regulate the levels of free intracellular zinc in the oocyte during maturation. In the cumulus-oocyte complex (COC) the relative level of free intracellular zinc was almost fourfold higher in cumulus cells compared with the resident germinal vesicle-stage oocyte. Removal of cumulus cells caused a fourfold increase in intracellular zinc in the oocyte by 1 h after cumulus cell removal, but subsequent coculture of denuded oocytes with COC decreased free intracellular zinc in the oocyte by 65%. Thus, cumulus cells suppress free intracellular zinc in the oocyte. The mRNA transcripts for the zinc transporter proteins Slc39a6, Slc39a8, Slc39a9, Slc39a10, Slc39a12, Slc30a2, Slc30a4, Slc30a5 and Slc30a8 mRNAs were higher in oocytes, while Slc39a1, Slc39a7, Slc39a13, Slc39a14, Slc30a6, Slc30a7 and Slc30a9 mRNAs were higher in cumulus cells. Thus a complex zinc transport network is present in the COC. Pretreatment with epidermal growth factor for 4 h abolished the ability of COCs to restrict free intracellular zinc in denuded oocytes. Coculture of denuded metaphase II oocytes with COC lowers free intracellular zinc in mature oocytes. Oocytes matured in vivo or oocytes from older mice had lower levels of free intracellular zinc than oocytes matured in vitro or from younger mice. Thus, a precise mechanism for regulating oocyte zinc homeostasis has been uncovered in the COC that is disrupted with increasing age or by removal of cumulus cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Zinc Homoeostasis In the Oocytementioning

confidence: 99%

Oocyte–cumulus cell interactions regulate free intracellular zinc in mouse oocytes

Lisle¹,

Anthony²,

Randall³

et al. 2013

Reproduction

View full text Add to dashboard Cite

show abstract

“…However, these frequencies were achieved to the cost of the transfer of a high number (3-5) of embryos, a sign that implantation rates were low in those cycles. In a recent retrospective case-control study involving patients with polycystic ovaries (Gremeau et al, 2012), Gremeau and colleagues were able to achieve a pregnancy rate of almost 20% by transferring much less embryos (on average 1.9), although in the same work it was also reported that implantation and pregnancy rates achieved in the same typology of patients was much higher in standard ovarian stimulation cases.…”

Section: Developmental Competence Of In Vitro Matured Human Oocytesmentioning

confidence: 99%

Human oocyte maturation in vitro

Coticchio¹,

Canto²,

Guglielmo³

et al. 2012

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Oocytes from medium-sized antral follicles have already completed their growth phase and, if released from the follicular environment and cultured in vitro, are able to resume the meiotic process and mature. However, in vitro maturation (IVM) does not entirely support all the nuclear and cytoplasmic changes that occur physiologically as an effect of the ovulatory stimulus. Regardless, oocyte IVM is widely applied for the breeding of agriculturally important species. In assisted reproduction technology, IVM has been proposed as an alternative treatment to circumvent the drawbacks of standard ovarian stimulation regimens. Initially introduced to eliminate the risks of ovarian hyperstimulation syndrome afflicting women presenting with polycystic ovaries, subsequently IVM has been suggested to represent an additional approach suitable also for normovulatory patients. So far, in children born from IVM cycles, no doubts of an increased incidence of congenital abnormalities have been raised. Many more births would be achieved if novel IVM systems, currently dominated by empiricism, could be conceived according to more physiological criteria. Recent findings shedding new light on the control of meiotic progression, the support of cumulus cells to the oocyte cellular reorganization occurring during maturation, and the modulation of the stimulus that promotes oocyte maturation downstream the mid-cycle gonadotropin signal are likely to provide crucial hints for the development of more efficient IVM systems.

show abstract

“…Retrieved oocytes are cultured in the laboratory and after they achieve maturity, oocytes are treated as in conventional IVF. Usually 60-80 % of oocytes will become mature and the fertilization rate is similar to that of conventional IVF [1,2,7,11,17]. Some studies suggest that successful pregnancy with IVM is correlated with the number oocytes retrieved [3].…”

Section: Introductionmentioning

confidence: 97%

A comparison of the Cook single lumen immature ovum IVM needle to the Steiner-Tan pseudo double lumen flushing needle for oocyte retrieval for IVM

Rose¹,

Laky²

2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose This study compared the impact of using the Steiner-Tan pseudo double lumen needle for antral follicle oocyte retrieval to using a conventional non-flushing needle. The Steiner-Tan needle has a much smaller dead space than the needles commonly used for IVM oocyte retrievals. Methods This was a retrospective cohort study. The patient population was determined by the time period in which a patient underwent IVM in a single physician's IVF practice. The following data was abstracted from clinical and embryology records: oocytes retrieved, oocytes matured, early maturing oocytes, oocytes fertilized, embryo quality measures, retrieval time, needle punctures, clot formation, and clinical pregnancy rate. Results The Steiner-Tan needle did not increase the number of oocytes retrieved. It also did not increase the time required for retrieval. However, flushing of antral follicles significantly decreased clot formation in fluid aspirates. Use of the Steiner-Tan needle also significantly decreased the number of vaginal needle punctures during each case. There was a trend toward improved embryo quality, but statistical power was inadequate to show a difference. Conclusions The primary benefit of the Steiner-Tan needle was on the embryological aspects of IVM. Decreased blood and blood clots in the aspirates made an IVM retrieval more like conventional IVF for the embryologist. The patient also experienced less tissue trauma without increasing anesthesia or surgical time. There was no improvement in the number of oocytes retrieved, but based on the results, we hypothesized that oocytes were more commonly retrieved from slightly large follicles than when using a routine needle.

show abstract

In vitro maturation or in vitro fertilization for women with polycystic ovaries? A case–control study of 194 treatment cycles

Cited by 136 publications

References 30 publications

Oocyte–cumulus cell interactions regulate free intracellular zinc in mouse oocytes

Oocyte–cumulus cell interactions regulate free intracellular zinc in mouse oocytes

Human oocyte maturation in vitro

A comparison of the Cook single lumen immature ovum IVM needle to the Steiner-Tan pseudo double lumen flushing needle for oocyte retrieval for IVM

Contact Info

Product

Resources

About