In vitro measurement of articular cartilage deformations in the intact human hip joint under load.

Armstrong, Cecil; Bahrani, A.S.; Gardner, D. L.

doi:10.2106/00004623-197961050-00016

Cited by 134 publications

(56 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, in a study by Armstrong et al, 1 articular cartilage deformation in subjects of the same age range as that of our subjects was on the order of 2% under loads of 5 times body weight. The applied forces in our study were 10% of the loads in the Armstrong et al 1 study, so we are relatively confident that articular cartilage deformation could not have been more than a negligible contribution to the femoroacetabular dis- tances measured in this study.…”

Section: Discussionsupporting

confidence: 43%

In Vivo Ultrasound Measurement of Posterior Femoral Glide During Hip Joint Mobilization in Healthy College Students

Loubert

Zipple²,

Klobucher³

et al. 2013

J Orthop Sports Phys Ther

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 43%

In Vivo Ultrasound Measurement of Posterior Femoral Glide During Hip Joint Mobilization in Healthy College Students

Loubert

Zipple²,

Klobucher³

et al. 2013

J Orthop Sports Phys Ther

View full text Add to dashboard Cite

“…The hydrogels, in fact, have an intrinsic stiffness due to the rigidity of the CS moieties. Unconfined compression strain of 5-20% (at a strain rate 1%/s) can be applied for the mechanical testing of cartilage tissue 11,12 since the physiological strain experienced by cartilage tissue under loading condition has been reported to be 10-20% 13,14 In conclusion, the use of the 3D biomimetic hydrogels to study the potential of different chondrocyte population to generate cartilage tissue can be widely applied. Besides the in vitro studies described here, in vivo transplantation of the cell-laden constructs can be envisioned to study cell maturation and regenerative potential in the physiological context.…”

Section: Discussionmentioning

confidence: 99%

“…2. Submerge the specimens in a PBS bath at RT and compress at a rate of 1% strain/sec to a maximum strain of 15% 11,12 since the physiological strain experienced by cartilage tissue under loading condition has been reported to be 10-20% 13,14 .…”

Section: Mechanical Testingmentioning

confidence: 99%

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Smeriglio

Lai

Yang

et al. 2015

JoVE

View full text Add to dashboard Cite

Human articular cartilage is highly susceptible to damage and has limited self-repair and regeneration potential. Cell-based strategies to engineer cartilage tissue offer a promising solution to repair articular cartilage. To select the optimal cell source for tissue repair, it is important to develop an appropriate culture platform to systematically examine the biological and biomechanical differences in the tissue-engineered cartilage by different cell sources. Here we applied a three-dimensional (3D) biomimetic hydrogel culture platform to systematically examine cartilage regeneration potential of juvenile, adult, and osteoarthritic (OA) chondrocytes. The 3D biomimetic hydrogel consisted of synthetic component poly(ethylene glycol) and bioactive component chondroitin sulfate, which provides a physiologically relevant microenvironment for in vitro culture of chondrocytes. In addition, the scaffold may be potentially used for cell delivery for cartilage repair in vivo. Cartilage tissue engineered in the scaffold can be evaluated using quantitative gene expression, immunofluorescence staining, biochemical assays, and mechanical testing. Utilizing these outcomes, we were able to characterize the differential regenerative potential of chondrocytes of varying age, both at the gene expression level and in the biochemical and biomechanical properties of the engineered cartilage tissue. The 3D culture model could be applied to investigate the molecular and functional differences among chondrocytes and progenitor cells from different stages of normal or aberrant development.

show abstract

“…The 15% compressive strain chosen here is within the physiological range for articular cartilage. 40 Finally, a dynamic shear test was performed (0.01-10 Hz) with a logarithmic frequency sweep at a shear strain of 0.01 radian. The complex shear modulus was calculated as follows:…”

Section: Scaffold Mechanical Propertiesmentioning

confidence: 99%

A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

Khanarian

Jiang

Wan

et al. 2012

Tissue Engineering Part A

109

View full text Add to dashboard Cite

Osteoarthritis is the leading cause of physical disability among Americans, and tissue engineered cartilage grafts have emerged as a promising treatment option for this debilitating condition. Currently, the formation of a stable interface between the cartilage graft and subchondral bone remains a significant challenge. This study evaluates the potential of a hybrid scaffold of hydroxyapatite (HA) and alginate hydrogel for the regeneration of the osteochondral interface. Specifically, the effects of HA on the response of chondrocytes were determined, focusing on changes in matrix production and mineralization, as well as scaffold mechanical properties over time. Additionally, the optimal chondrocyte population for interface tissue engineering was evaluated. It was observed that the HA phase of the composite scaffold promoted the formation of a proteoglycan-and type II collagen-rich matrix when seeded with deep zone chondrocytes. More importantly, the elevated biosynthesis translated into significant increases in both compressive and shear moduli relative to the mineral-free control. Presence of HA also promoted chondrocyte hypertrophy and type X collagen deposition. These results demonstrate that the hydrogel-calcium phosphate composite supported the formation of a calcified cartilage-like matrix and is a promising scaffold design for osteochondral interface tissue engineering.

show abstract

In vitro measurement of articular cartilage deformations in the intact human hip joint under load.

Cited by 134 publications

References 0 publications

In Vivo Ultrasound Measurement of Posterior Femoral Glide During Hip Joint Mobilization in Healthy College Students

In Vivo Ultrasound Measurement of Posterior Femoral Glide During Hip Joint Mobilization in Healthy College Students

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

Contact Info

Product

Resources

About