In vitro measurement of rate of fluid secretion in rat isolated seminiferous tubules: effects of metabolic inhibitors and ions.

Cheung, Yuen-Ming; Hwang, Jin-Taek; Wong, Patrick

doi:10.1113/jphysiol.1977.sp011889

Cited by 20 publications

(16 citation statements)

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“…Although previous studies employing efferent duct ligation in intact rat testes (Setchell, 1967) and micropuncture and microcannulation of the rat seminiferous tubules in vivo (Tuck et al 1970;Levinè & Marsh, 1971) and in vitro (Cheung et al 1977) have provided evidence that the seminiferous tubules elaborate a watery secretion into the tubular lumen, the mechanism of secretion and its regulation have not been fully explored. This study represents a first attempt to unravel secretory mechanisms by measuring electrogenic ion transport across cultured monolayers of rat Sertoli cells.…”

Section: Discussionmentioning

confidence: 99%

Regulated anion secretion in cultured epithelia from Sertoli cells of immature rats

Chan

Chew

et al. 1998

The Journal of Physiology

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Cultured epithelia of Sertoli cells from prepubertal rats were grown on Matrigel‐coated millipore filters for short‐circuit current (Isc) measurements. Under basal conditions, these epithelia exhibited a ‘zero’ transepithelial potential difference, a ‘zero’ short‐circuit current and a transepithelial resistance of 60 Ω cm2. Forskolin (100 μm) and 8‐(4‐chlorophenylthio)‐cAMP (cpt‐cAMP) (100 μm) added to the apical side stimulated the Isc (forskolin, peak ΔIsc= 1.32 ± 0.16 μA cm−1; cpt‐cAMP, peak ΔIsc= 0.88 ± 0.16 μA cm−2). ATP (100 μm) added apically elicited a Isc response (peak ΔIsc= 6.45 ± 0.28 μA cm−2) which was similar in magnitude to that of 1 μm thapsigargin (peak ΔIsc= 6.09 ± 0.44 μA cm−2). The potency of the responses to other nucleotides: UTP ≥ ATP > ADP >> AMP = adenosine indicates the involvement of a mixture of P2Y receptors. Removal of extracellular Cl− and HCO3− reduced the Isc response to ATP by 70 % and 40 %, respectively. Removal of K+ had no effect, whereas removal of Na+ attenuated the Isc response. The response to ATP was insensitive to agents known to block anion secretion (except apical diphenylamine‐2‐carboxylate (DPC) and DIDS). The resistance to perturbation by pharmacological agents may be a unique property of the seminiferous epithelium. Whole‐cell current recordings in cultured rat Sertoli cells demonstrated a DIDS‐sensitive outwardly rectifying Cl− conductance with activating and inactivating characteristics at depolarizing and hyperpolarizing voltages, respectively. The stimulation of electrogenic ion transport by ATP may be part of a complex mechanism regulating fluid secretion by the testis. Cultured Sertoli cell epithelia are shown to provide a useful model to investigate transepithelial transport in the seminiferous epithelium.

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Section: Discussionmentioning

confidence: 99%

Regulated anion secretion in cultured epithelia from Sertoli cells of immature rats

Chan

Chew

et al. 1998

The Journal of Physiology

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“…Ace reduces in vitro¯uid secretion by the seminiferous tubules [5] and under in vivo conditions it decreases¯uid secretion in the rete testis [30]. Additional¯uid reabsorption appears to take place along the epididymis, resulting in a marked concentration of sperm [18,36].…”

Section: Discussionmentioning

confidence: 99%

“…When Ace, the prototype of CA inhibitors, is added in vitro to isolated seminiferous tubules,¯uid secretion is reduced [5]. Similarly, when administered in vivo to rams,¯uid secretion in the rete testis is reduced [30].…”

mentioning

confidence: 99%

Influence of Acetazolamide on Aqp1 Gene Expression in Testis and on Sperm Count/Motility in Epididymis of Rats

Sun

Bai

et al. 2002

Archives of Andrology

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“…A segment of the cauda epididymidis (about 0.3 cm long) was dissected free of fat and connective tissue and placed on a specially designed platform similar to that used for measuring secretory rate in isolated rat seminiferous tubules (9). Each end was clamped onto the platform, and incisions were made in the duct close to the clamps.…”

Section: Methodsmentioning

confidence: 99%

Effects of Catecholamines and Adrenergic Blockade on Fluid Reabsorption in Isolated Rat Cauda Epididymidis

Wong

Yeung

1978

Japanese Journal of Pharmacology

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Abstract-The rate of fluid reabsorption in the cauda epididymidis of rat has been measured in vitro.Both adrenaline and isoprenaline produced a prompt, reversible and dose dependent increase in the reabsorption rate. These effects were completely blocked by propranolol.The response to noradrenaline consisted of two components. I n the presence of an alpha blocker, noradrenaline caused an increase, while in the presence of a beta blocker, it produced an inhibition in the rate of fluid reabsorption. The effects of these adrenergic agents were only observed when sodium ions were present in the intraluminal fluid, suggesting that they only affect the Na+-dependent component of fluid reabsorption.The possibility that they may affect the active transport of sodium in the duct was discussed.The effects of these adrenergic agents were inter preted in terms of the presence of alpha and beta receptors in the epithelium of the rat cauda epididymidis.It is now well established that fluid reabsorption takes place along the entire length of the mammalian epididymal duct. A major part of the rete testis fluid is reabsorbed as it flows down the epididymis (1, 2, 3). The mechanism of fluid reabsorption has been studied in isolated duct of rat epididymis in vitro. It was found that 50 % of the fluid reabsorption is secondary to an active transepithelial transport of sodium ions (4, 5). Furthermore this process, like many epididymal functions, is abolished by castration, indicating that the fluid reabsorption process is dependent upon the presence of circulating androgens (6).There is little information on the role of neurotransmitters in the functions of the epididymis, although it has been shown that the cauda epididymidis is heavily innervated by sympathetic and parasympathetic nerves (7,8). In this paper, we report the effects of adrenergic agents and blockers on fluid reabsorption in isolated rat cauda epididymidis.The results were interpreted in terms of the presence of the alpha and beta adrenergic re ceptors in the epididymal epithelium. MATERIALS AND METHODSMale Sprague-Dawley rats weighing between 200-300 g were sacrificed by a blow on the head. The epididymis was quickly removed and placed in cold Kreb's bicarbonate solution.A segment of the cauda epididymidis (about 0.3 cm long) was dissected free of fat and connective tissue and placed on a specially designed platform similar to that used for measuring secretory rate in isolated rat seminiferous tubules (9). Each end was clamped onto the platform, and incisions were made in the duct close to the clamps. The lumen was

show abstract

In vitro measurement of rate of fluid secretion in rat isolated seminiferous tubules: effects of metabolic inhibitors and ions.

Cited by 20 publications

References 19 publications

Regulated anion secretion in cultured epithelia from Sertoli cells of immature rats

Regulated anion secretion in cultured epithelia from Sertoli cells of immature rats

Influence of Acetazolamide on Aqp1 Gene Expression in Testis and on Sperm Count/Motility in Epididymis of Rats

Effects of Catecholamines and Adrenergic Blockade on Fluid Reabsorption in Isolated Rat Cauda Epididymidis

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