In vitro Membrane Stabilizing and In vivo Analgesic Activities of Boehmeria glomerulifera Miq. in Swiss-Albino Mice Model

Abstract

Boehmeria glomerulifera Miq. is an indigenous plant of Bangladesh. Traditional healers use this plant to treat various diseases. The crude methanol extract of whole plant of B. glomerulifera and its Kupchan fractions were screened for in vitro membrane stabilizing and only the crude extract at doses of 200-and 400-mg/kg body weight were screened for analgesic activity in mice model in order to establish the medicinal values of this plant. The membrane stabilizing activity of the extractives was evaluated by the ability of test materials to inhibit hypotonic solution-and heat-induced haemolysis of human erythrocytes. Moreover, the analgesic activity of methanol extract was evaluated by acetic acid induced writhing method and tail immersion method in mice. In hypotonic solution-and heat-induced conditions, the crude methanol extract showed inhibition of haemolysis by 64.80±0.34% and 21.63±0.76%, respectively as compared to 77.74±0.67% and 40.41±0.69% demonstrated by the standard, acetyl salicylic acid. On the other hand, the analgesic activity was determined for its peripheral and central pharmacological responses using acetic acid-induced writhing test and tail immersion method at doses of 200-and 400-mg/kg body weight. The extract reduced the acetic acid-induced writhings significantly (p < 0.05) in a dose dependent manner with the highest activity observed at 400 mg/kg (46.0%) body weight when compared to that of Diclofenac sodium (64.0%), as the standard drug. A significant increase (p < 0.01) of latency period was also found in tail immersion method. Therefore, the present study established that the methanol extract of B. glomerulifera possesses moderate membrane stabilizing and significant analgesic activities.

Peripheral Analgesic and Anti-diarrheal Activities of Leaf of Syzygium cumini (L.) Skeel

“…Peripheral analgesic activity: Total 48 mice of both sexes were randomly divided into 8 groups of 6 mice each. By employing the acetic acid-induced writhing method (Koster et al, 1959;Ahmed et al, 2001;Papia et al, 2016;Razan et al, 2016;Faruk et al, 2015), the peripheral analgesic activity of the samples was assessed in mice. The negative control group received 1% Tween-80 solution in normal saline.…”

In Silico Screening of Hispolon and its Analogs: Pharmacokinetics and Molecular Docking Studies with Cyclooxygenase-2 Enzyme

“…World Health Organization (WHO) published a report in 2008 stating over 80% of the world's populace depends on traditional medicine for their primary healthcare needs [2]. So, traditional medicines serve as important sources of new drugs or drug candidates [3] and more than 80% of drug candidates are either directly obtained from natural products or developed from natural compounds [4]. About 50% pharmaceuticals are derived from substances first identified or isolated from natural sources such as herbs/plants, organisms, animals, and insects [5].…”