In vitro metabolic stress induced by nitazoxanide and flubendazole combination in Taenia crassiceps cysticerci

Lima, Nayana Ferreira de; Picanço, Guaraciara de Andrade; Costa, Tatiane Luiza da; Vinaud, Marina Clare

doi:10.1016/j.exppara.2022.108265

Cited by 3 publications

(2 citation statements)

References 28 publications

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Section: Resultsmentioning

confidence: 99%

“…In vitro studies have employed Taenia crassiceps (ORF strain cysticerci) as a closely related experimental model species to T. solium [19,20]. Standard cell culture involves the cultivation of T. crassiceps cysticerci in RPMI medium [21]. In order to elucidate the mechanisms of T. solium oncosphere attachment to host intestinal tissues, Verastegui et al [22] established in vitro models using three substrates: porcine mucosal scrapings with mucin; porcine mucosal explants; and monolayers of CHO-K1 (Chinese hamster ovary) cells.…”

Section: Taenia Soliummentioning

confidence: 99%

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The Truman Show for Human Helminthic Parasites: A Review of Recent Advances in In Vitro Cultivation Platforms

Sutrave

Richter

2023

Microorganisms

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Throughout history, parasites and parasitic diseases have been humankind’s constant companions, as evidenced by the findings of tapeworm eggs in ancient, mummified remains. Helminths are responsible for causing severe, long-term, and debilitating infectious diseases worldwide, especially affecting economically challenged nations due to prevailing deficits in access to sanitation, proper hygiene practices, and healthcare infrastructure. Socio-ecological drivers, such as poverty, migration, and climate change, continue to contribute to parasites and their disease vectors being spread beyond known endemic zones. The study of parasitic diseases has had a fair amount of success leading to the development of new chemotherapeutic agents and the implementation of parasite eradication programs. However, further progress in this direction has been hampered by the challenges of culturing some of these parasites in in vitro systems for efficient availability, basic life cycle, infection studies, and effectiveness of novel treatment strategies. The complexity of the existing models varies widely, depending on the parasite and its life cycle, ranging from basic culture methods to advanced 3D systems. This review aims to highlight the research conducted so far in culturing and maintaining parasites in an in vitro setting, thereby contributing to a better understanding of pathogenicity and generating new insights into their lifecycles in the hopes of leading to effective treatments and prevention strategies. This work is the first comprehensive outline of existing in vitro models for highly transmissible helminth diseases causing severe morbidity and mortality in humans globally.

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Section: Resultsmentioning

confidence: 99%