In vitro metabolism of methiocarb and carbaryl in rats, and its effect on their estrogenic and antiandrogenic activities

Tange, Satoko; Fujimoto, Nariaki; Uramaru, Naoto; Wong, Fung Fuh; Sugihara, Kazumi; Ohta, Shigeru; Kawa, Shigeyuki

doi:10.1016/j.etap.2015.08.014

Cited by 23 publications

(13 citation statements)

References 37 publications

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“…In contrast to the neonicotinoids, the insecticide Methiocarb induced a significant estrogenic effect mediated by estrogen receptors (effect observed starting from 3.91 × 10 −6 M). Similar results were also shown by three previous studies [ 25 , 33 , 34 ]. Andersen et al [ 25 ] found a significant estrogenic effect testing this pesticide using E-screen assay on MCF-7 BUS cells and a gene reporter assay on transfected MCF-7 BUS cells (effect observed starting from 10 −5 M and 5 × 10 −6 M, respectively).…”

Section: Discussionsupporting

confidence: 93%

Assessment of Five Pesticides as Endocrine-Disrupting Chemicals: Effects on Estrogen Receptors and Aromatase

Gea¹,

Zhang

Tota³

et al. 2022

IJERPH

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Pesticides are widely applied all over the world, and pesticide exposure can induce different biological effects posing a possible threat to human health. Due to their effects on the endocrine system, some pesticides are classified as endocrine disruptors. The aim of the study is to assess the interference of five pesticides on estrogen biosynthesis and estrogen signaling. Three neonicotinoid insecticides (Acetamiprid, Clothianidin, and Thiamethoxam), a carbamate insecticide (Methiocarb) and a herbicide (Oxadiazon) were tested. The effect of pesticides on estrogen biosynthesis was studied through an ELISA assay using a recombinant form of human aromatase, the enzyme that catalyzes the transformation of androgens to estrogens. Moreover, the effect of pesticides on estrogen signaling was assessed using a gene reporter assay on MELN cells, which measures estrogen receptor-mediated estrogenic activity. The results of the ELISA assay showed that the pesticides did not alter aromatase activity (no interference with estrogen biosynthesis), while the results of the gene reporter assay showed that only Methiocarb was able to alter estrogen signaling at high doses. The estrogenic activity of Methiocarb, expressed as 17β-estradiol equivalency factor (EEF), was equal to 8.0 × 10−8. In conclusion, this study suggested that Methiocarb should be considered a potential endocrine disruptor.

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Section: Discussionsupporting

confidence: 93%

Assessment of Five Pesticides as Endocrine-Disrupting Chemicals: Effects on Estrogen Receptors and Aromatase

Gea¹,

Zhang

Tota³

et al. 2022

IJERPH

View full text Add to dashboard Cite

show abstract

“…Methiocarb, a carbamate that is used as a bird repellent, insecticide and molluscicide, is a possible differential diagnosis 3,27,28 . It acts as an acetylcholinesterase inhibitor, resulting in cholinergic stimulation 29 and is supplied as a green‐colored bait 30 . A toxicological examination was not performed in all patients, therefore methiocarb intoxication cannot be ruled out completely.…”

Section: Discussionmentioning

confidence: 99%

Retrospective evaluation of the use of hemodialysis in dogs with suspected metaldehyde poisoning (2012–2017): 11 cases

Teichmann‐Knorrn

Doerfelt

2020

J Vet Emergen Crit Care

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Objective To evaluate dogs treated with hemodialysis for severe metaldehyde intoxication and to compare them with conventionally‐managed patients. Design Retrospective study (2012–2017). Setting University teaching hospital. Animals Data from 18 dogs with severe metaldehyde intoxication were analyzed. Eleven dogs were treated with intermittent hemodialysis and 7 managed conventionally. Interventions None. Measurement and main results Metaldehyde poisoning was diagnosed based on clinical signs and toxicological analysis or presence of turquoise material in the gastrointestinal content. Clinical signs, additional treatments, dialysis prescription, duration of anesthesia and hospitalization, complications, and outcome were documented. Results were analyzed by t‐test, Mann–Whitney U‐test, and Chi‐square test. P < 0.05 was considered significant. Dialysis was performed for median (range) 180 min (150–420 min) with median of 2.28 L/kg (1.66–4.48 L/kg) blood volume processed. In the hemodialysis group, anesthesia was discontinued at a median of 3.0 hours (1.5–6.7 h) after starting dialysis. The conventional‐group received general anesthesia for a median of 17.5 hours (7.0–30.5 h). No further anticonvulsive treatment was necessary for the hemodialysis group. Time to hospital discharge was shorter in dialyzed dogs (median 18 h; 15–41 h) compared to conventionally treated dogs (median 89 h; 61–168 h; P = 0.0014). Aspiration pneumonia was reported in 5 conventionally treated dogs and none of the dialyzed dogs (P = 0.001). Five dialyzed dogs developed hematoma at the dialysis catheter site. One dog in each group was euthanized. Conclusion Hemodialysis significantly decreases the requirement for anesthesia and length of hospitalization in dogs with metaldehyde intoxication. Aspiration pneumonia occurred less often in dialyzed patients. Prospective studies are warranted to confirm the clinical utility of hemodialysis in dogs with metaldehyde poisoning.

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“…Carbaryl and methiocarb (and their hydrolysis products) were shown to exhibit in vitro ERα and ERβ agonistic activity. Furthermore, these compounds exhibited antiandrogenic activity ( Tange et al, 2016 ). Pirimicarb, another carbamate pesticide, has been reported to decrease ERα mRNA levels ( Grünfeld and Bonefeld-Jorgensen, 2004 ).…”

Section: Toxicity Of Carbamatesmentioning

confidence: 99%

Conserved Metabolic and Evolutionary Themes in Microbial Degradation of Carbamate Pesticides

2021

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Carbamate pesticides are widely used as insecticides, nematicides, acaricides, herbicides and fungicides in the agriculture, food and public health sector. However, only a minor fraction of the applied quantity reaches the target organisms. The majority of it persists in the environment, impacting the non-target biota, leading to ecological disturbance. The toxicity of these compounds to biota is mediated through cholinergic and non-cholinergic routes, thereby making their clean-up cardinal. Microbes, specifically bacteria, have adapted to the presence of these compounds by evolving degradation pathways and thus play a major role in their removal from the biosphere. Over the past few decades, various genetic, metabolic and biochemical analyses exploring carbamate degradation in bacteria have revealed certain conserved themes in metabolic pathways like the enzymatic hydrolysis of the carbamate ester or amide linkage, funnelling of aryl carbamates into respective dihydroxy aromatic intermediates, C1 metabolism and nitrogen assimilation. Further, genomic and functional analyses have provided insights on mechanisms like horizontal gene transfer and enzyme promiscuity, which drive the evolution of degradation phenotype. Compartmentalisation of metabolic pathway enzymes serves as an additional strategy that further aids in optimising the degradation efficiency. This review highlights and discusses the conclusions drawn from various analyses over the past few decades; and provides a comprehensive view of the environmental fate, toxicity, metabolic routes, related genes and enzymes as well as evolutionary mechanisms associated with the degradation of widely employed carbamate pesticides. Additionally, various strategies like application of consortia for efficient degradation, metabolic engineering and adaptive laboratory evolution, which aid in improvising remediation efficiency and overcoming the challenges associated with in situ bioremediation are discussed.

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In vitro metabolism of methiocarb and carbaryl in rats, and its effect on their estrogenic and antiandrogenic activities

Cited by 23 publications

References 37 publications

Assessment of Five Pesticides as Endocrine-Disrupting Chemicals: Effects on Estrogen Receptors and Aromatase

Assessment of Five Pesticides as Endocrine-Disrupting Chemicals: Effects on Estrogen Receptors and Aromatase

Retrospective evaluation of the use of hemodialysis in dogs with suspected metaldehyde poisoning (2012–2017): 11 cases

Conserved Metabolic and Evolutionary Themes in Microbial Degradation of Carbamate Pesticides

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