In vitro micropropagation of almond (Amygdalus communis L. cv. Nonpareil)

Işıkalan, Çiğdem; Akbas, FA; Namli, Sureyya; Tilkat, Engin; Başaran, Davut

doi:10.5897/ajb2008.000-5035

Cited by 11 publications

(2 citation statements)

References 15 publications

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“…In a study conducted on the effect of different concentrations of sucrose (10,20,30,40, and 50 g L -1 ) added to the MS medium with the aim of multiplication of papaya (Carica papaya L.) shoots. It was noted that the concentration of 30 gm L -1 sucrose was significantly superior to the rest of the sucrose concentrations in terms of the high response to shoot multiplication.…”

Section: Effect Of Carbon Source On Tissue Culturementioning

confidence: 99%

“…Nodule stem explants of moringa (Moringa oleifera L.) plant that were grown in MS medium + 1 mg L -1 BA and 0.2 mg L -1 NAA recorded the highest response rate to shoot proliferation and the number of shoots reached 68.33% and 4.91 shoots, respectively [19]. The highest number of roots and longest root length/plantlets were seen when the concentration of indole acetic acid was 8.0 mg L -1 [30]. In a study conducted, it was observed that the addition of 1.0 and 1.5 mg L -1 of benzylaminopurine (BAP) in combination with 1.0 mg L -1 of indole-3-butyric acid (IBA) resulted in a survival rate of 96.7% for MM 106 apple plantlets and 93.3% for Anna apple plantlets [26].…”

Section: Effect Of Plant Hormones On Tissue Culturementioning

confidence: 99%

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Advancement in Tissue Culture Techniques for Fruit Crops

Jain,

Sharma,

Nidhi

et al. 2023

IJECC

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Tissue culture is a highly promising approach that enables the efficient propagation of many plants from tiny fragments of the parent plant within a relatively brief timeframe and confined area. Tissue culture, a contemporary approach, is primarily employed for the efficient and extensive replication of many commercially significant plant species, such as the date palm. Utilizing the tissue culture technique presents a potential approach for generating a substantial quantity of genetically homogeneous palm plants that resemble other plants and yield typical fruit within four years from initial planting. Furthermore, this technique allows to produce date palm plants devoid of diseases, exhibiting an exceptionally high survival rate of nearly 100% when compared to the traditional vegetative propagation of shoots, owing to the robustness of their root system. The process of surface sterilization holds significant importance in the production of explants for in vitro studies, as it effectively addresses the issue of bacterial and fungal contamination originating from field sources, which might vary considerably across different fruit plant species. The efficacy of tissue culture techniques for date palm acclimatization in vitro is contingent upon the observation of leaf count prior to transplantation in the greenhouse. Hence, the primary objective of this study was to investigate the determinants that govern the tissue culture of fruit trees. India is known for being the native land of various fruit crops that are both significant and minor in terms of their importance. These crops include Indian gooseberry (Emblica officinalis Gaertn.), Karonda (Carissa carandas L.), Bael (Aegle marmelos Corr.), Jamun (Syzygium cuminii L.), and jackfruit (Artocarpus heterophyllus L.), etc. These fruits possess considerable nutritional, medicinal, and therapeutic value, making them highly valuable in commercial sectors such as medicine, food, and cosmetics. The limited availability of suitable planting materials imposes constraints on the commercial production process for these crops. Using plant tissue culture techniques holds promise in substantially augmenting the number of novel cultivars or genotypes inside fruit crops. The primary aim of this review study is to consolidate and synthesize the extant body of knowledge about the tissue culture techniques employed in cultivating various fruit crops.

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Section: Effect Of Carbon Source On Tissue Culturementioning

confidence: 99%

Section: Effect Of Plant Hormones On Tissue Culturementioning

confidence: 99%

Advancement in Tissue Culture Techniques for Fruit Crops

Jain,

Sharma,

Nidhi

et al. 2023

IJECC

View full text Add to dashboard Cite

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Developing a Promising Micropropagation Method for Several Drought Tolerant and Hard-to-Root Wild and Domesticated Almond Genotypes by Shoot Tips Culture

et al. 2023

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Recovery of virus-free Almond (Prunus dulcis) cultivars by somatic embryogenesis from meristem undergone thermotherapy

Ebrahimi

Habashi

Emadpour

et al. 2022

Sci Rep

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One of the world's main horticulture problems is the contamination of fruit trees with a variety of plant diseases, especially viral and pseudo-viral diseases. Due to the non-sexual propagation of the trees, these diseases have been transmitted to different parts of the world. The main aim of this study was to obtain a new effective method for virus elimination from almond cultivars, which was performed in two phases. In the first phase, we tested various almond cultivars with ELISA and RT-PCR. The results showed the infection of mother plantlets. So, three types of in vitro thermotherapy treatments were performed on infected plants to make them virus-free. The plantlets obtained from 0.5 mm meristem treated with the first type of thermotherapy (TH1: 8 h at 27 °C and 16 h at 38 °C for 18 days) showed the highest percentage of elimination of ApM, ACLS and TRS viruses. In the second phase, meristems were cultured on MS medium containing 0, 0.5, 1 and 2 mg/L 2,4-D with 1 mg/L TDZ and after two weeks, thermotherapy treatments were performed. The results showed, combining three methods of thermotherapy (TH1), meristem culture and somatic embryogenesis induction from meristem on MS medium supplemented with 0.5 mg/L 2,4-D and 1 mg/L TDZ is the most effective and safe technique for virus eradication without meristem size challenges. The samples that were diagnosed as virus-free were proliferated in temporary immersion bioreactor systems, and rooted to be used for later propagation and establishment of mother healthy orchards.

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In vitro micropropagation of almond (Amygdalus communis L. cv. Nonpareil)

Cited by 11 publications

References 15 publications

Advancement in Tissue Culture Techniques for Fruit Crops

Advancement in Tissue Culture Techniques for Fruit Crops

Developing a Promising Micropropagation Method for Several Drought Tolerant and Hard-to-Root Wild and Domesticated Almond Genotypes by Shoot Tips Culture

Recovery of virus-free Almond (Prunus dulcis) cultivars by somatic embryogenesis from meristem undergone thermotherapy

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