In vitro Micropropagation of Desmodium gangeticum (L.) DC (Fam-Fabaceae): A Medicinal Legume through Axillary Bud Multiplication

Puhan, Puspashree; Rath, S. P.

doi:10.3923/pjbs.2012.477.483

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…2 B). BAP was also reported as suitable phytohormone for multiple shoot induction/proliferation/ multiplication from in vivo node in a number of medicinal legumes like like Cassia tora (Quraishi et al, 2011), Desmodium gangeticum (Puhan and Rath, 2012) and Dalbergia sissoo (Vibha et al, 2014) respectively.…”

Section: Evaluation Of Growth Regulators For Shoot Induction Multipli...mentioning

confidence: 99%

Plant Regeneration Through Axillary Shoot Proliferation of Canavalia Gladiata (Jacq) . Dc., an Economically Important Underutilized Legume- A Step Forward for the Reduction of Anti-Nutritional Factors (Anfs).

Senapati¹,

Barik²,

Moharana³

2023

PLANT ARCHIVES

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Canavalia gladiata (Jacq) DC., is a legume and considered as a good source for protein because of presence of quality protein similar to most edible food legumes and can be used as food and feed. The seeds of C. gladiata are known for several valuable phytochemicals, useful for food and health benefits properties. Still the plant is underutilized/ not consumed worldwide due to presence of some anti-nutritional factors (ANFs) such as Concavalin A, Protease inhibitor, tannins, flatulence factors, allergens and lysinoalanine phytates and L-canavine etc particularly in the seed. So, it is necessary to remove all these anti-nutritional compounds to eligible the seed of C. gladiata as food for human society to combat the nutritional deficiency. To increase the nutritional value as well to reduce anti-nutritional compounds from the seed different kind of processes such as sprouting, roasting, fermentation, de-hulling, cooking etc. have been adopted. But the above methods are not efficient enough to reduce anti-nutritional compounds. Therefore, there is a need to develop plantlets with null or reduced anti-nutritional compounds. Tissue culture method is a first and necessary strategy for any kind of further research regarding the reduction of anti-nutritional factors. Whether it is by gene editing or alteration in any regeneration influencing factors during micropropagation anything can be possible by the exploration of tissue culture technique. So, in this part of work, a simple, efficient and reliable micropropagation protocol for Canavalia gladiata (Jacq.) DC was developed by using in vivo nodal segment for the first time. The nodal segment of 3 rd -6 th position are found suitable for multiple shoot regeneration. The optimum shoot proliferation has been found in Murashige and Skoog's (MS) medium supplemented with BAP 2.0 mg/L showing 100% response with 5.66 average shoots/explant of average length 3.90 cm. For upscaling purpose, the in vitro nodes used as explant from primary shoots harvested from in vivo node. Again, MS medium with BAP 2.0 mg/L found as optimum medium for these in vitro nodes having 100% response with average shoot/ explant 2.00 of average length 4.96 cm. The seasonal influence on micropropagation has also studied where, Dec-Jan was observed as best season for explant collection showing 92% bud break followed by Feb-March. Half-MS supplemented with IBA 0.5 mg/L found as optimum medium (100% response) for rooting of in vitro regenerated shoots with average 4.0 roots/shoot and average root length 3.45 cm. The plantlet was acclimatized on a simple mixture of sand: soil (1: 1) and subsequently transferred to the garden under natural condition. Micropropagated plant developed through this protocol can be a step forward for further research work in reducing its anti-nutritional factor.

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Section: Evaluation Of Growth Regulators For Shoot Induction Multipli...mentioning

confidence: 99%

Plant Regeneration Through Axillary Shoot Proliferation of Canavalia Gladiata (Jacq) . Dc., an Economically Important Underutilized Legume- A Step Forward for the Reduction of Anti-Nutritional Factors (Anfs).

Senapati¹,

Barik²,

Moharana³

2023

PLANT ARCHIVES

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Influence of physio-chemical factors on high throughput plant regeneration and micro-morpho-anatomy of shoots of Ormocarpum sennoides (Willd.) DC

2021

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Tissue culture tools for selenium hyperaccumulator Neptunia amplexicaulis for development in phytoextraction

O’Donohue

Hiti-Bandaralage

Gleeson

et al. 2022

Nat. Prod. Bioprospect.

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Neptunia amplexicaulis is an herbaceous legume endemic to the Richmond area in central Queensland, Australia and is one of the strongest known Selenium hyperaccumulators on earth, showing significant potential to be utilised in Se phytoextraction applications. Here a protocol was established for in vitro micropropagation of Se hyperaccumulator N. amplexicaulis using nodal segments from in vitro-germinated seedlings. Shoot multiplication was achieved on Murashige and Skoog (MS) basal media supplemented with various concentrations of 6-Benzylaminopurine (BA) (1.0, 2.0, 3.0 mg L−1) alone or in combination with low levels of Naphthaleneacetic acid (NAA) (0.1, 0.2, 0.3 mg L−1), with 2.0 mg L−1 BA + 0.2 mg L−1 NAA found to be most effective. Elongated shoots were rooted in vitro using NAA, with highest root induction rate of 30% observed at 0.2 mg L−1 NAA. About 95% of the in vitro rooted shoots survived acclimatization. Clonally propagated plantlets were dosed with selenate/selenite solution and assessed for Se tissue concentrations using Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES) and found to retain their ability to hyperaccumulate. The protocol developed for this study has potential to be optimised for generating clonal plants of N. amplexicaulis for use in research and phytoextraction industry applications.

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In vitro Micropropagation of Desmodium gangeticum (L.) DC (Fam-Fabaceae): A Medicinal Legume through Axillary Bud Multiplication

Cited by 3 publications

References 7 publications

Plant Regeneration Through Axillary Shoot Proliferation of Canavalia Gladiata (Jacq) . Dc., an Economically Important Underutilized Legume- A Step Forward for the Reduction of Anti-Nutritional Factors (Anfs).

Plant Regeneration Through Axillary Shoot Proliferation of Canavalia Gladiata (Jacq) . Dc., an Economically Important Underutilized Legume- A Step Forward for the Reduction of Anti-Nutritional Factors (Anfs).

Influence of physio-chemical factors on high throughput plant regeneration and micro-morpho-anatomy of shoots of Ormocarpum sennoides (Willd.) DC

Tissue culture tools for selenium hyperaccumulator Neptunia amplexicaulis for development in phytoextraction

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