In vitro micropropagation of<i>Catharanthus roseus</i>— An anticancer medicinal plant

Bakrudeen, Abdul; Shanthi, G. Subha; Gouthaman, T.; Kavitha, M.; Rao, M. S.

doi:10.1556/abot.53.2011.1-2.20

Cited by 14 publications

(9 citation statements)

References 18 publications

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“…These differences in shoot formation may be due to the different genotypes and explant sources. Kinetin also promotes multiple shoot production in C. roseus [19,24,26,27]. The percentage of shoot formation (59.4%), the number of shoots (5.7), and shoot elongation (3.6 cm) on MS medium with 4 µM kinetin were higher than in the other kinetin treatments and the control (Table 1).…”

Section: Discussionmentioning

confidence: 93%

“…Explants with vegetative meristems are often suitable for axillary shoot multiplication and clonal propagation. Direct multiple shoot regeneration has been achieved using nodal segments, shoot tips, and axillary buds from C. roseus seedlings and mature plants [11,20,[24][25][26][27][28]. Cytokinins play an essential role in shoot development.…”

Section: Introductionmentioning

confidence: 99%

“…Cytokinins play an essential role in shoot development. N 6 -benzyladenine (BA) [19,[25][26][27], N 6 -furfuryladenine (Kinetin) [19,24,26,27], and thidiazuron (1-phenyl-3-(1,2,3,-thiadiazol-5-yl)urea, TDZ) [19] are used to induce multiple shoots in C. roseus. TDZ (substituted phenyl urea) is more efficient at multiple shoot formation in several shrubs, including C. roseus [19,29,30].…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Lee

Zengin

et al. 2020

Molecules

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This study aimed to investigate the impact of plant growth regulators, sucrose concentration, and the number of subcultures on axillary shoot multiplication, in vitro flowering, and somaclonal variation and to assess the phytochemical composition, antioxidant capacity, and enzyme inhibitory potential of in vitro-established callus, somaclonal variant, and normal green shoots of Catharanthus roseus. The highest shoot induction rate (95.8%) and highest number of shoots (23.6), with a mean length of 4.5 cm, were attained when the C. roseus nodal explants (0.6–1 cm in length) were cultivated in Murashige and Skoog (MS) medium with 2 µM thidiazuron, 1 µM 2-(1-naphthyl) acetic acid (NAA), and 4% sucrose. The in vitro flowering of C. roseus was affected by sucrose, and the number of subcultures had a significant effect on shoot multiplication and somaclonal variation. The highest levels of phenolics and flavonoids were found in normal green shoots, followed by those in somaclonal variant shoots and callus. The phytochemicals in C. roseus extracts were qualified using liquid chromatography–tandem mass spectrometry. A total of 39, 55, and 59 compounds were identified in the callus, somaclonal variant shoot, and normal green shoot tissues, respectively. The normal green shoot extracts exhibited the best free radical scavenging ability and reducing power activity. The strongest acetylcholinesterase inhibitory effects were found in the callus, with an IC50 of 0.65 mg/mL.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Lee

Zengin

et al. 2020

Molecules

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“…The increase in demands of this plant for commercial use requires an alternative rate of proliferation. During these years, in vitro technique is being widely applied to produce identical quality and disease-free plants [ 16 ]. There is no evidence on propagation of C. latifolia from seeds, but we reported that it is being propagated through rhizome [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Screening of Antioxidant and Antibacterial Activities and Establishment of an Efficient Callus Induction in Curculigo latifolia Dryand (Lemba)

Farzinebrahimi

Taha

Rashid

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Leaf, seed, and tuber explants of C. latifolia were inoculated on MS medium supplemented with various concentrations of BAP and IBA, alone or in combinations, to achieve in vitro plant regeneration. Subsequently, antioxidant and antibacterial activities were determined from in vitro and in vivo plant developed. No response was observed from seed culture on MS media with various concentrations of PGRs. The highest percentage of callus was observed on tuber explants (94%) and leaf explants (89%) when cultured on MS media supplemented with IBA in combination with BAP. A maximum of 88% shoots per tuber explant, with a mean number of shoots (8.8 ± 1.0), were obtained on MS medium supplemented with combinations of BAP and IBA (2.5 mg L−1). The best root induction (92%) and mean number (7.6 ± 0.5) from tuber explants were recorded on 2.5 mg L−1 IBA alone supplemented to MS medium. The higher antioxidant content (80%) was observed from in vivo tuber. However, tuber part from the intact plant showed higher inhibition zone in antibacterial activity compared to other in vitro and in vivo tested parts.

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“…Modern researches have confirmed that the secondary metabolites are being used as anti-cancer, antitumor, anti-fungal and cytotoxic agents (Nishino et al, 1988). Tissue culture and micropropagation of plants have been done by Jullani et al, (1999), Lippia junelliana., Rout et al, (2000), Gupta et al, (2001), Lippia alba., Been and Martin (2003) in Ceropegia candelabrum., Bhavisha and Jasrai (2003), Arumugan et al, (2003), Teli and Tinko (2004), Lee (2005), Aziz et al, (2006), Zhou and Wu (2006), Arya et al, (2008), Kayo (2008), Balaraju et al, (2008), Sasikumar et al, (2009), Singh et al, (2009), Sidhu (2010), Ahmad et al, (2011), Bakrudeen et al, (2011), Parven and Shahzad (2011), Priya and Ravindra (2011), Arumugan and Gopinath (2012), Dagla (2012), Garg and Malik (2012), Thiyagrajan and Venkatachalam (2012), Vidya (2012), Balaji and Ebnezer (2013), Daksh et al, (2014), Cristiano (2015), and Kavita and Gopal (2018). Keeping the importance of this plant, present study was carried out for in vitro micropropagation of Phyla nodiflora (Lippia nodiflora L.).…”

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confidence: 99%

OPTIMIZATION OF CONCENTRATIONS OF PLANT GROWTH REGULATORS FOR In vitro MULTIPLE SHOOT FORMATION AND EFFICIENT ROOT INDUCTION IN Phyla nodiflora L. (Lippia nodiflora L.) AN IMPORTANT MEDICINAL PLANT

Kumari¹,

Priya²

2020

Indian Journal of Scientific Research

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In the present study nodal and shoot tip explants were cultured in MS medium supplemented with different concentrations of BAP and KN separately, which were fortified with three different concentrations of NAA. It was noted that when nodal explants was inoculated in MS+ 2.0 mg/l BAP and 1.5 mg/l NAA, the percentage of response for shoot bud induction was 88.84 and the number of shoots per explants in this medium was 9.64. These were the highest percentage of response and the number shoots in this medium. In the same culture condition the percentage response for shoot tip explants was 82.26, while the number of shoots buds per explants was 7.25, respectively. When nodal explants were inoculated in MS+ 2.0 mg/l KN + 1.5 mg/l NAA, the percentage response for shoot bud induction was 75.25 and umber of shoots per explants in this medium was 6.39, respectively. In the same medium, when the shoot tip explant was inoculated, the percentage of response for shoot bud initiation was 70.36 and number of shoots per explant was 4.75 respectively. It was further noted that although the above explants, also responded to other concentrations of BAP and KN, along with NAA in MS basal medium but there was discrepancy in both the percentage response as well as the number of shoot buds initiated per explants. Above in vitro raised and well grown plantlets were used for rooting. For this both ½ strength as well ¼ strength MS basal medium were supplemented with seven different concentrations of IAA and IBA separately. Shoots were excised and inoculated in the aforesaid rooting medium. Here again it was noted that the explants said rooting medium. Here again it was noted that the explants inoculated in ½ MS + 1.0 mg/l IBA revealed maximum percentage (73.54) after 36 th days of inoculation. The number of roots per explants was 34. ½ MS + 1.0 mg/l IAA, induced roots and the percentage of response was 66.36, while the number of roots per explants was 2.6 only. It may be concluded that for shoot bud induction, nodal explant and MS + 2.0 mg/l BAP + 1.5 mg/l IBA was most promising culture condition. Similarly, for root induction ½ MS + 1.0 mg/l IBA was the best medium for Phyla nodiflora.

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In vitro micropropagation ofCatharanthus roseus— An anticancer medicinal plant

Cited by 14 publications

References 18 publications

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Preliminary Screening of Antioxidant and Antibacterial Activities and Establishment of an Efficient Callus Induction in Curculigo latifolia Dryand (Lemba)

OPTIMIZATION OF CONCENTRATIONS OF PLANT GROWTH REGULATORS FOR In vitro MULTIPLE SHOOT FORMATION AND EFFICIENT ROOT INDUCTION IN Phyla nodiflora L. (Lippia nodiflora L.) AN IMPORTANT MEDICINAL PLANT

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