In vitro microspore selection in maize anther culture with oxidative-stress stimulators

Ambrus, H.; Darkó, Éva; Szabó, László; Bakos, Ferenc; Király, Zoltán; Barnabás, Beáta

doi:10.1007/s00709-006-0159-1

Cited by 15 publications

(16 citation statements)

References 30 publications

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“…To conclude, the plant‐specific glutaredoxins ROXY1 and ROXY2 require a conserved glycine interacting with GSH for modification of target proteins. Environmental stress is known to decrease plant fertility, and gametophyte development is highly sensitive to stress and reactive oxygen species (Ambrus et al. , 2006; Sun et al.…”

Section: Discussionmentioning

confidence: 99%

“…To conclude, the plant-specific glutaredoxins ROXY1 and ROXY2 require a conserved glycine interacting with GSH for modification of target proteins. Environmental stress is known to decrease plant fertility, and gametophyte development is highly sensitive to stress and reactive oxygen species (Ambrus et al, 2006;Sun et al, 2004). Mutagenesis studies have suggested that conserved cysteines in MYB R2R3 proteins mediate their redox-dependent ability to bind to promoter sequences (Heine et al, 2004), and thus these proteins might represent targets for post-translational modification by ROXY1 and ROXY2.…”

Section: A Model For Roxy1/2 Function In Anther Developmentmentioning

confidence: 99%

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ROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for anther development

2007

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SummaryGlutaredoxins (GRXs) are small oxidoreductases that are involved in various cellular processes and play a crucial role in responses to oxidative stress. Three GRX subgroups exist in plants, and GRXs with active sites of the CPYC and CGFS types are common to pro-and eukaryotes. In contrast, GRXs with the CC type motif have so far only been identified in land plants. Here, we report that the two CC-type GRXs ROXY1 and ROXY2 together control anther development in Arabidopsis thaliana. Single roxy1 and roxy2 mutants are fertile and produce normal anthers. However, roxy1 roxy2 double mutants are sterile and do not produce pollen. Strikingly, abaxial and adaxial anther lobe differentiation are differently affected, with early lobe differentiation being defective in the adaxial lobes, whereas later steps during pollen mother cell differentiation are disrupted in the abaxial lobes. Expression studies show that ROXY1 and ROXY2 are expressed with overlapping patterns during anther development. Lack of ROXY1 and ROXY2 function affects a large number of anther genes at the transcriptional level. Genetic and RT-PCR data imply that ROXY1/2 function downstream of the early-acting anther gene SPOROCYTELESS/NOZZLE and upstream of DYSFUNCTIONAL TAPETUM1, controlling tapetum development. Mutagenesis of a conserved glutathione-binding glycine in the ROXY1 protein indicates that CC-type GRXs need to interact with glutathione to catalyze essential biosynthetic reactions. Analysis of these two novel anther genes indicates that redox regulation, as well as participating in plant stress defense mechanisms, might play a major role in the control of male gametogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: A Model For Roxy1/2 Function In Anther Developmentmentioning

confidence: 99%

ROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for anther development

2007

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“…In a cytoplasmic male sterile line of rice the oxidative stress originating in mitochondria was found to be associated with premature abortion of microspores in the early stage of pollen development [34]. As well, the treatment of maize anthers with the ROS progenitors resulted in abnormal cell divisions and progeny cell degradation during the development of the microspores [35]. Similarly, the development of fertilized egg to an embryo has been found to be sensitive to a multitude of environmental factors.…”

Section: Discussionmentioning

confidence: 99%

Melatonin and serotonin in flowers and fruits of Datura metel L.

Murch

Alan

Cao

et al. 2009

Journal of Pineal Research

173

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Datura metel is a plant that contains several different neurologically active phytochemicals which affect human health. On-going research has examined the potential role of the human neuroindoles, melatonin and serotonin, in medicinal plants with neurological efficacy. In this report, we describe the quantification of melatonin and serotonin in flowers and developing fruits of Datura metel and the effects of cold stress on the levels of these neuroindoles in the reproductive tissues of this plant. Melatonin and serotonin were found at the highest levels in the least developed flower buds with decreasing concentrations as the flower buds matured. Cold stress significantly increased the concentration of melatonin in young flower buds. In the developing fruit, melatonin was present at relatively stable, high concentrations for the first 10 days after anthesis. After 10-15 days, the ovule had grown to a sufficient size for excision and analysis and melatonin was found to be at the highest concentrations in the developing ovule with minimal concentrations of the neuroindoles in the fleshy fruit. Together, these data indicate that melatonin may play a role in protecting the reproductive tissues during flower and seed formation in a Datura species.

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“…We compared two cultivation methods using two culture media N6 (Chu 1981) and YP in our experiments. N6 and YP media are the most often used media for microspore initiation in maize anther culture (Ting et al 1981;Genovesi and Collins 1982;Petolino and Jones 1986;Tsay et al 1986;Petolino et al 1988;Barloy et al 1989;Büter et al 1993;Barnabás et al 1999;Nageli et al 1999;Aulinger et al 2003;Jäger et al 2005;Ambrus et al 2006;Pret'ová et al 2006). A direct comparison of both media has not been performed yet.…”

Section: Discussionmentioning

confidence: 97%

Enzyme fingerprint analyses in tissue regenerated from anther culture of maize

et al. 2008

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The objectives of our studies were to investigate the effect of cold pre-treatment duration and the effect of two different culture media (YP and N6) on maize anther culture response in two maize genotypes (A 18 and A 19) and to identify the gametic origin of the maize regenerants. Androgenic induction and callus formation was compared in anther cultures following pre-treatment applied to both media tested and with both maize genotypes. Higher plant regeneration was observed in case of YP media independently of the genotype used. The best results were achieved when 12 days (genotype A 18) or 14 days (in case of genotype A 19) cold pre-treatment at 10°C was applied. We have tested the possibility of using enzyme isoform analyses to identify the microspore origin of calli and plants derived from anther cultures. The 11 enzymes tested in our experiments were acid phosphatase, alcohol dehydrogenase, catalase, diaphorase, b-glucosidase, glutamate oxaloacetate transaminase, isocitrate dehydrogenase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase and phosphoglucoisomerase. Analysis of malate dehydrogenase proved the gametic origin of the calli initiated and of the DH plants regenerated from anther culture, when the coleoptile of the donor plant material showed two forms of enzyme 3/6 and the analysed calli showed only one of the two forms (3 or 6).

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In vitro microspore selection in maize anther culture with oxidative-stress stimulators

Cited by 15 publications

References 30 publications

ROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for anther development

ROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for anther development

Melatonin and serotonin in flowers and fruits of Datura metel L.

Enzyme fingerprint analyses in tissue regenerated from anther culture of maize

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