In vitro model of infected stratum corneum for the efficacy evaluation of poloxamer 407-based formulations of ciclopirox olamine against Trichophyton rubrum as well as differential scanning calorimetry and stability studies

Täuber, Anja; Müller‐Goymann, Christel C.

doi:10.1016/j.ijpharm.2015.08.023

Cited by 10 publications

(4 citation statements)

References 16 publications

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“… 29 Other techniques designed to separate the membrane at the dermal–epidermal junction include chemical treatments with ethylenediaminetetraacetic acid, ammonia, and enzymes. 32 Some researchers have used the isolated SC for permeation experiments 36 and for desorption studies designed to study SC heterogeneity, 37 and the SC reservoir for water and other substances. 38 The SC is prepared by enzymatic methods, usually by incubation with aqueous trypsin solution, after which the digested epidermal material is rinsed and wiped off.…”

Section: Models Used To Evaluate Dermal Absorptionmentioning

confidence: 99%

Skin models for the testing of transdermal drugs

Abd¹,

Yousef²,

Pastore³

et al. 2016

CPAA

232

227

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The assessment of percutaneous permeation of molecules is a key step in the evaluation of dermal or transdermal delivery systems. If the drugs are intended for delivery to humans, the most appropriate setting in which to do the assessment is the in vivo human. However, this may not be possible for ethical, practical, or economic reasons, particularly in the early phases of development. It is thus necessary to find alternative methods using accessible and reproducible surrogates for in vivo human skin. A range of models has been developed, including ex vivo human skin, usually obtained from cadavers or plastic surgery patients, ex vivo animal skin, and artificial or reconstructed skin models. Increasingly, largely driven by regulatory authorities and industry, there is a focus on developing standardized techniques and protocols. With this comes the need to demonstrate that the surrogate models produce results that correlate with those from in vivo human studies and that they can be used to show bioequivalence of different topical products. This review discusses the alternative skin models that have been developed as surrogates for normal and diseased skin and examines the concepts of using model systems for in vitro–in vivo correlation and the demonstration of bioequivalence.

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Section: Models Used To Evaluate Dermal Absorptionmentioning

confidence: 99%

Skin models for the testing of transdermal drugs

Abd¹,

Yousef²,

Pastore³

et al. 2016

CPAA

232

227

View full text Add to dashboard Cite

show abstract

“…Although earlier studies using DSC were focused on studying the mechanical or biophysical properties of skin and its basic composition [11][12][13][14], the technique remains popular and continues to be employed in studies of cosmetic ingredients [15][16][17][18] and liposome-assisted drug delivery [19][20][21][22][23][24], where DSC serves as a primary tool in characterizing the matrix state, with polymorphism and drug incorporation in lipid dispersions [3].…”

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

Review of Modern Techniques for the Assessment of Skin Hydration

Qassem

Kyriacou

2019

Cosmetics

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Skin hydration is a complex process that influences the physical and mechanical properties of skin. Various technologies have emerged over the years to assess this parameter, with the current standard being electrical probe-based instruments. Nevertheless, their inability to provide detailed information has prompted the use of sophisticated spectroscopic and imaging methodologies, which are capable of in-depth skin analysis that includes structural and composition details. Modern imaging and spectroscopic techniques have transformed skin research in the dermatological and cosmetics disciplines, and are now commonly employed in conjunction with traditional methods for comprehensive assessment of both healthy and pathological skin. This article reviews current techniques employed in measuring skin hydration, and gives an account on their principle of operation and applications in skin-related research.

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“…Ciclopirox olamine (CPX), an antifungal agent commonly used for the dermatologic treatment of mycoses in clinical practice for more than 30 years [13, 14], has been shown recently to have antitumor properties in multiple cancers [15], including hematological malignancies, such as acute leukemia and multiple myeloma [16–19]. Yet, there is rare report on CPX’s antileukemia effect on ALL, especially on ALL with GC resistance.…”

Section: Introductionmentioning

confidence: 99%

Antileukemia Effect of Ciclopirox Olamine Is Mediated by Downregulation of Intracellular Ferritin and Inhibition β-Catenin-c-Myc Signaling Pathway in Glucocorticoid Resistant T-ALL Cell Lines

Liu

et al. 2016

PLoS ONE

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Ciclopirox olamine (CPX) is an antifungal drug that has been reported to have antitumor effects. In this study we investigated the antileukemia effects and the possible mechanisms of CPX on glucocorticoid (GC)-resistant T-cell acute lymphoblastic leukemia (T-ALL) cell lines. The results indicated that CPX inhibited the growth of GC-resistant T-ALL cells in a time- and dose-dependent manner, and this effect was closely correlated with the downregulation of intracellular ferritin. CPX induced cell cycle arrest at G1 phase by upregulation of cyclin-dependent kinase (CDK) inhibitor of p21 and downregulation of the expressions of cyclin D, retinoblastoma protein (Rb), and phosphorylated Rb (pRb). CPX also enhanced apoptotic cell death by downregulation of anti-apoptotic proteins such as Bcl-2, Bcl-xL, and Mcl-1. More importantly, CPX demonstrated a strong synergistic antileukemia effect with GC and this effect was mediated, at least in part, by inhibition of the β-catenin-c-Myc signaling pathway. These findings suggest that CPX could be a promising antileukemia drug, and modulation of the intracellular ferritin expression might be an effective method in the treatment of ALL. Therefore, integrating CPX into the current GC-containing ALL protocols could lead to the improvement of the outcome of ALL, especially GC-resistant ALL.

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In vitro model of infected stratum corneum for the efficacy evaluation of poloxamer 407-based formulations of ciclopirox olamine against Trichophyton rubrum as well as differential scanning calorimetry and stability studies

Cited by 10 publications

References 16 publications

Skin models for the testing of transdermal drugs

Skin models for the testing of transdermal drugs

Review of Modern Techniques for the Assessment of Skin Hydration

Antileukemia Effect of Ciclopirox Olamine Is Mediated by Downregulation of Intracellular Ferritin and Inhibition β-Catenin-c-Myc Signaling Pathway in Glucocorticoid Resistant T-ALL Cell Lines

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