In vitro modelling of the physiological and diseased female reproductive system

Stejskalová, Anna; Vankelecom, Hugo; Sourouni, Marina; Ho, Magdalene Y; Götte, Martin; Almquist, Benjamin D.

doi:10.1016/j.actbio.2021.04.032

Cited by 17 publications

(12 citation statements)

References 350 publications

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“…Previous 2D cell culture studies have shown that Sdc‐1 plays a role in cancer progression of IBC 11,18,19 . However, 2D cell culture models harbour some disadvantages, exposing cells to unphysiologically high stiffness, non‐natural environments, and a limited directionality of cell–cell interactions 25 . We therefore decided to study a possible impact of Sdc‐1‐depletion on the progression of DCIS in a matrigel‐based 3D cell culture model.…”

Section: Resultsmentioning

confidence: 99%

“…11,18,19 However, 2D cell culture models harbour some disadvantages, exposing cells to unphysiologically high stiffness, nonnatural environments, and a limited directionality of cell-cell interactions. 25 We therefore decided to study a possible impact of Sdc-1-depletion on the progression of DCIS in a matrigel-based 3D cell culture model. For this purpose, Sdc-1 siRNA knockdown was induced in the DCIS model cell line MCF10A DCIS.com via siRNA-transfection.…”

Section: Syndecan-1-knockdown Promotes Invasion Of Mcf10a Dciscom Cel...mentioning

confidence: 99%

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The heparan sulphate proteoglycan Syndecan‐1 (CD138) regulates tumour progression in a 3D model of ductal carcinoma in situ of the breast

et al. 2022

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Ductal carcinoma in situ (DCIS) is a form of breast cancer that is restricted to the lactiferous ducts and has not yet invaded the surrounding breast tissue.Dysregulation of the transmembrane heparan sulphate proteoglycan Syndecan-1 (Sdc-1) plays a role in tumour progression of invasive breast cancer (IBC). In DCIS, Sdc-1, c-Met and E-cadherin are part of a proangiogenic expression signature. In this study, we employed a siRNA knockdown approach in the DCIS model cell line MCF10A DCIS.com to investigate a potential connection between Sdc-1 and epithelial mesenchymal transition (EMT), proteolysis and the Rho kinase pathway. Analysis of gene expression data of the TNMplot.com database revealed that Sdc-1 expression was higher in primary breast tumours compared to metastases. The impact of Sdc-1-depletion on the cellular phenotype was investigated in a Matrigel-based three-dimensional cell culture model. Sdc-1 depletion resulted in the formation of larger spheroids and the formation of invasive protrusions. Application of matrix metalloproteinase (MMP) and Rho kinase inhibitors could block the Sdc-1-induced phenotype. qPCR analysis of Sdc-1-depleted cells in twodimensional culture revealed upregulated expression of the EMT-markers CDH1, FN-1, CLDN1, the proteolysis markers MMP3, and MMP9, and HPSE, while MMP2, VIM and ROCK-2 were downregulated. Immunocytochemistry confirmed upregulation of MMP9 and fibronectin, the latter being particular prominent after ROCK inhibition. STRING analysis confirmed an interaction

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Section: Resultsmentioning

confidence: 99%

Section: Syndecan-1-knockdown Promotes Invasion Of Mcf10a Dciscom Cel...mentioning

confidence: 99%

The heparan sulphate proteoglycan Syndecan‐1 (CD138) regulates tumour progression in a 3D model of ductal carcinoma in situ of the breast

et al. 2022

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“…The pathophysiology of endometriosis is multifactorial; a selection of pathways is reported below. The historical lack of endometriosis models makes the identification of new targets and therapeutic agents challenging; however, in vitro [21][22][23][24][25][26][27][28] and in vivo [29][30][31] models of endometriosis are emerging. Table 1 shows a selection of active principles in different stages of clinical and preclinical development for endometriosis treatment, some of which were reviewed recently.…”

Section: Molecular Targets and Active Principles In Endometriosismentioning

confidence: 99%

Endometriosis, the Silent Disease: Molecular Targets, Active Principles, and Drug Delivery Systems

Teworte

Luciani

2022

Helvetica Chimica Acta

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In this perspective, we provide a broad overview of pathophysiological processes involved in endometriosis, including the role of inflammation, fibrosis, angiogenesis, hormonal regulation, and genetic factors. For each of the functional changes involved with the etiology of the disease, we showcase a selection of active principles in various stages of preclinical and clinical development. Finally, we highlight the role of drug delivery for endometriosis, particularly oral vs. vaginal administration. In doing so, we wish to draw the community's attention to this debilitating disease and highlight the need for interdisciplinary approaches to endometriosis treatment development.

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“…The physiological roles of endometrial epithelial and stromal cells, as well as the genesis of numerous pathologic diseases such as endometrial cancer, are well understood. Endometrial organoids will never have the context they need to be true mini organs until they have a fuller complement of cell types (Stejskalová et al, 2021).…”

Section: Endometrial Organoidsmentioning

confidence: 99%

“…The inaccessible luminal compartment and apical appearance of the epithelium of organoids make physiologically relevant research difficult. A luminal compartment might be created by unfolding the spherical organoid into a 2D planar tissue construct and monolayer culture of primary epithelial cells from organoids (Stejskalová et al, 2021).…”

Section: Endometrial Organoidsmentioning

confidence: 99%

In vitro 3D Model of Female Reproductive Tract: An Overview and Future Aspect

Capuzzo¹

2021

Preprint

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Hormones must be balanced and dynamically controlled for the Female Reproductive Tract (FRT) to function correctly during the menstrual cycle, pregnancy, and delivery. Gamete selection and successful transfer to the uterus, where it implants and pregnancy occurs, is supported by the mucosal epithelial lining of the FRT ovaries, uterus, cervix, fallopian tubes, and vagina. Successful implantation and placentation in humans and other animals rely on complex interactions between the embryo and a receptive female reproductive system. The FRT's recent breakthroughs in three-dimensional (3D) organoid systems now provide critical experimental models that match the organ's physiological, functional, and anatomical characteristics in vitro. This article summarizes the current state of the art on organoids generated from various parts of the FRT. The current analysis examines recent developments in the creation of organoid models of reproductive organs, as well as their future directions.

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In vitro modelling of the physiological and diseased female reproductive system

Cited by 17 publications

References 350 publications

The heparan sulphate proteoglycan Syndecan‐1 (CD138) regulates tumour progression in a 3D model of ductal carcinoma in situ of the breast

The heparan sulphate proteoglycan Syndecan‐1 (CD138) regulates tumour progression in a 3D model of ductal carcinoma in situ of the breast

Endometriosis, the Silent Disease: Molecular Targets, Active Principles, and Drug Delivery Systems

In vitro 3D Model of Female Reproductive Tract: An Overview and Future Aspect

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