In vitro models for the assessment of inflammatory and immuno-modulatory effects of the volatile organic compound chlorobenzene

Lehmann, Irina; Röder-Stolinski, Carmen; Nieber, K; Fischäder, Gundula

doi:10.1016/j.etp.2008.01.009

Cited by 37 publications

(20 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This phenomenon could be another reason for the extremely early and rapid macrophage accumulation in ATP-vesicle treated wounds because of the reciprocal recruitment [6] MCP-1 is also a strong stimulator of angiogenesis [73,74]. T cells treated with MCP-1 show enhanced production of IL-4 and IL-13 cytokines [75,76] whereas MCP-1 deficient mice cannot increase their Th2 response [77]. …”

Section: Discussionmentioning

confidence: 99%

Rapid tissue regeneration induced by intracellular ATP delivery—A preliminary mechanistic study

et al. 2017

View full text Add to dashboard Cite

We have reported a new phenomenon in acute wound healing following the use of intracellular ATP delivery—extremely rapid tissue regeneration, which starts less than 24 h after surgery, and is accompanied by massive macrophage trafficking, in situ proliferation, and direct collagen production. This unusual process bypasses the formation of the traditional provisional extracellular matrix and significantly shortens the wound healing process. Although macrophages/monocytes are known to play a critical role in the initiation and progression of wound healing, their in situ proliferation and direct collagen production in wound healing have never been reported previously. We have explored these two very specific pathways during wound healing, while excluding confounding factors in the in vivo environment by analyzing wound samples and performing in vitro studies. The use of immunohistochemical studies enabled the detection of in situ macrophage proliferation in ATP-vesicle treated wounds. Primary human macrophages and Raw 264.7 cells were used for an in vitro study involving treatment with ATP vesicles, free Mg-ATP alone, lipid vesicles alone, Regranex, or culture medium. Collagen type 1α 1, MCP-1, IL-6, and IL-10 levels were determined by ELISA of the culture supernatant. The intracellular collagen type 1α1 localization was determined with immunocytochemistry. ATP-vesicle treated wounds showed high immunoreactivity towards BrdU and PCNA antigens, indicating in situ proliferation. Most of the cultured macrophages treated with ATP-vesicles maintained their classic phenotype and expressed high levels of collagen type 1α1 for a longer duration than was observed with cells treated with Regranex. These studies provide the first clear evidence of in situ macrophage proliferation and direct collagen production during wound healing. These findings provide part of the explanation for the extremely rapid tissue regeneration, and this treatment may hold promise for acute and chronic wound care.

show abstract

Section: Discussionmentioning

confidence: 99%

Rapid tissue regeneration induced by intracellular ATP delivery—A preliminary mechanistic study

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Human monocytes in vitro and in tumor environments can be induced by MCP-1 to undergo M2 polarization (34). T cells treated with MCP-1 elaborate IL-4 and IL-13 (Th2 cytokines) (35, 36) and MCP-1-deficient mice cannot mount Th2 responses (37). Some of the influence of MCP-1 on the promotion of Th2 responses may be mediated through MCP-1-induced protein-1 (MCPIP1), a down regulator of inflammatory responses that degrades cytokine mRNAs and deubiquitinates TNF receptor-associated factor family proteins (38, 39), thus allowing an unfettered Th2 response.…”

Section: Discussionmentioning

confidence: 99%

Th1/M1 Conversion to Th2/M2 Responses in Models of Inflammation Lacking Cell Death Stimulates Maturation of Monocyte Precursors to Fibroblasts

Trial¹,

Cieslik²,

Haudek³

et al. 2013

Front. Immunol.

View full text Add to dashboard Cite

We have demonstrated that cardiac fibrosis arises from the differentiation of monocyte-derived fibroblasts. We present here evidence that this process requires sequential Th1 and Th2 induction promoting analogous M1 (classically activated) and M2 (alternatively activated) macrophage polarity. Our models are: (1) mice subjected to daily repetitive ischemia and reperfusion (I/R) without infarction and (2) the in vitro transmigration of human mononuclear leukocytes through human cardiac microvascular endothelium. In the mouse heart, leukocytes entered after I/R in response to monocyte chemoattractant protein-1 (MCP-1), which is the major cytokine induced by this protocol. Monocytes within the heart then differentiated into fibroblasts making collagen while bearing the markers of M2 macrophages. T cells were seen in these hearts as well as in the human heart with cardiomyopathy. In the in vitro model, transmigration of the leukocytes was likewise induced by MCP-1 and some monocytes matured into fibroblasts bearing M2 markers. In this model, the MCP-1 stimulus induced a transient Th1 and M1 response that developed into a predominantly Th2 and M2 response. An increase in the Th2 product IL-13 was present in both the human and the mouse models, consistent with its known role in fibrosis. In these simplified models, in which there is no cell death to stimulate an anti-inflammatory response, there is nonetheless a resolution of inflammation enabling a profibrotic environment. This induces the maturation of monocyte precursors into fibroblasts.

show abstract

“…Therefore, it is thought that the IL-8 may be upregulated by proinflammatory compounds and oxidants. Therefore, we chose chlorobenzene [31], sodium sulfite [32], and sphingosine-1-phosphate [33] as proinflammatory compounds, and paraquat [34] as an oxidant (Table 3). …”

Section: Resultsmentioning

confidence: 99%

A Novel Approach for a Toxicity Prediction Model of Environmental Pollutants by Using a Quantitative Structure-Activity Relationship Method Based on Toxicogenomics

et al. 2011

View full text Add to dashboard Cite

The development of automobile emission reduction technologies has decreased dramatically the particle concentration in emissions; however, there is a possibility that unexpected harmful chemicals are formed in emissions due to new technologies and fuels. Therefore, we attempted to develop new and efficient toxicity prediction models for the myriad environmental pollutants including those in automobile emissions. We chose 54 compounds related to engine exhaust and, by use of the DNA microarray, examined their effect on gene expression in human lung cells. We focused on IL-8 as a proinflammatory cytokine and developed a prediction model with quantitative structure-activity relationship (QSAR) for the IL-8 gene expression by using an in silico system. Our results demonstrate that this model showed high accuracy in predicting upregulation of the IL-8 gene. These results suggest that the prediction model with QSAR based on the gene expression from toxicogenomics may have great potential in predictive toxicology of environmental pollutants.

show abstract

In vitro models for the assessment of inflammatory and immuno-modulatory effects of the volatile organic compound chlorobenzene

Cited by 37 publications

References 18 publications

Rapid tissue regeneration induced by intracellular ATP delivery—A preliminary mechanistic study

Rapid tissue regeneration induced by intracellular ATP delivery—A preliminary mechanistic study

Th1/M1 Conversion to Th2/M2 Responses in Models of Inflammation Lacking Cell Death Stimulates Maturation of Monocyte Precursors to Fibroblasts

A Novel Approach for a Toxicity Prediction Model of Environmental Pollutants by Using a Quantitative Structure-Activity Relationship Method Based on Toxicogenomics

Contact Info

Product

Resources

About