In vitro models for the evaluation of angiogenic potential in bone engineering

Cenni, E.; Perut, Francesca; Baldini, Nicola

doi:10.1038/aps.2010.143

Cited by 31 publications

(26 citation statements)

References 120 publications

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“…Recently, membrane platforms were used for paracrine signaling study, but these studies suffer from major drawbacks, such as the lack of in vivo like functionality, separation of specific cells of interest and low pore density, micrometer‐scale film thickness, and optical non‐transparency of the membrane. [1c] On the contrary, direct cell coculture approach offers more in vivo like cell–cell communication environment, but this method also has many issues including cell cross contamination by xenogenic reaction and difficulties in manipulating and isolating each cell line for detailed individual analysis …”

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Transparent, Nanoporous, and Transferable Membrane‐Based Cell–Cell Paracrine Signaling Assay

et al. 2015

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signaling molecules from each cell line separately. Recently, membrane platforms were used for paracrine signaling study, but these studies suffer from major drawbacks, such as the lack of in vivo like functionality, separation of specifi c cells of interest and low pore density, micrometer-scale fi lm thickness, and optical non-transparency of the membrane. [ 1c ] On the contrary, direct cell coculture approach offers more in vivo like cell-cell communication environment, [ 6 ] but this method also has many issues including cell cross contamination by xenogenic reaction and diffi culties in manipulating and isolating each cell line for detailed individual analysis. [ 7 ] Herein, we designed and prepared cellulose acetate (CA)-based nanoporous thin fi lm as a transparent, nanoporous, and transferable (TNT) cell coculture membrane to address the aforementioned issues in cell coculture assays. The TNT membrane allows for cell adhesion within a short time and separation of single cell layer effi ciently after coculturing of multiple cell lines through good affi nity to cells and transferability of membrane, which can provide a new platform for controlling and analyzing cell-cell paracrine signaling. CA has been widely used as fi lter membranes due to its low static charges and high mechanical strength. [ 8 ] Also, CA has received great attention in biomedical fi elds as cell scaffold materials that exhibit a wide range of properties, such as high modulus, adequate fl exural, and tensile strength with biocompatibility. [ 9 ] The porous structure of the CA membrane was realized by nonsolvent (water) vapor-induced phase separation (NVIPS) during spin coating in a closed chamber with controlled relative humidity (RH) ( Figure 1 a, and Figure S1, Supporting Information). In cell culture medium, the transparency, fl exibility, and facile transferability of the CA-based TNT membrane originate from its nmscale dimension in fi lm thickness (482 ± 7 nm), small pore size (≤150 nm), and low water solubility (Figure 1 b). To the best of our knowledge, this work is the fi rst example in adopting spincasted ultrathin CA membranes with controlled pore size and density for cell coculture and paracrine signaling assays. The TNT membrane allows accurate analysis of paracrine communication between cocultured cells as well as easy isolation of each cell line for further manipulation of individual cell lines. Also, the high degree of freedom in stacking and destacking of the membranes enable us not only to coculture three or more different cell lines simultaneously but also to coculture cell lines in a sequentially different manner, which would offer a highly versatile in vitro platform to trace the history of stromal activations for metastatic cancer cells.In a typical proof-of-concept experiment, MDA-MB-231 (metastatic cancer cell; M) and human mesenchymal stem cell (hMSC; H) were fi rst grown separately on a TNT membrane and cocultured by stacking the cell-culture TNT membranes Cells communicate with one another typically through di...

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confidence: 99%

Transparent, Nanoporous, and Transferable Membrane‐Based Cell–Cell Paracrine Signaling Assay

et al. 2015

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“…110,111 Addition of various growth factors like BMPs, platelet-rich plasma, VEGF, and hormones like estrogen (based on its antiapoptotic properties), PTH, or vitamin D3 to the osteoblast culture may prove to be useful. 3,6,32,[112][113][114] Cheng et al reported that while BMP-2, 6, and 9 could play an important role in prompting osteoblastic differentiation of MSCs, most BMPs would stimulate osteogenesis in mature osteoblasts. 22 A study by Balasch has reported increased proliferation of osteoblasts and MSCs even with low doses of oestradiol.…”

Section: Applications In Bone Tissue Bioengineeringmentioning

confidence: 99%

“…Osteoblasts in bone tissue engineering are areas of intense research. 14,15,23,58,113,158 Also, the issue of biosafety in tissue engineering when osteoblast-like cells are used persists, as it is reported that these cells may eventually show numerous chromosomal abnormalities. 159 These issues, however, are all being addressed by researchers, as described above.…”

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Osteoblasts and their applications in bone tissue engineering

Rupani¹,

Balint²,

Cartmell³

2012

CHC

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Tissue engineering is an emerging therapy that offers a new solution to patients suffering from bone loss. It utilizes cells derived from such sources as a patient's own bone or bone marrow, which are laboratory-isolated, grown (so they multiply in number), and placed onto a degradable material, or scaffold, that has mechanical/chemical properties appropriate to the bone section that it is replacing. The cells plus the scaffold are then grown in a container, or bioreactor, which is necessary as it provides the correct environment required for the cells to proliferate, differentiate, and to produce extracellular matrix. The following review focuses on the use of osteoblasts for bone tissue engineering.

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“…Adequate vascularization of tissue‐engineered constructs is an important prerequisite and could be promoted by inducing stem cells or progenitor cells to reach the site of injury/replacement for tissue repair (Lapidot et al ., ; Pelosi et al ., ). The development of new approaches leading to fast and successful vascularization is therefore one of the most intensively studied subjects in the field of tissue engineering and regenerative medicine (Moon and West, ; Cenni et al ., ). Our previous studies demonstrated that endothelial progenitor cells from human peripheral blood or cord blood are a potential autologous cell source for cellular therapies aimed at enhancing the neovascularization of tissue‐engineered constructs (Fuchs et al ., ; Ghanaati et al ., ; Kolbe et al ., ; Dohle et al ., ).…”

Section: Introductionmentioning

confidence: 97%

TLR4 stimulation by LPS enhances angiogenesis in a co-culture system consisting of primary human osteoblasts and outgrowth endothelial cells

Dohle

et al. 2015

J Tissue Eng Regen Med

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In vitro models for the evaluation of angiogenic potential in bone engineering

Cited by 31 publications

References 120 publications

Transparent, Nanoporous, and Transferable Membrane‐Based Cell–Cell Paracrine Signaling Assay

Transparent, Nanoporous, and Transferable Membrane‐Based Cell–Cell Paracrine Signaling Assay

Osteoblasts and their applications in bone tissue engineering

TLR4 stimulation by LPS enhances angiogenesis in a co-culture system consisting of primary human osteoblasts and outgrowth endothelial cells

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