In Vitro Models of Brain Disorders

Feber, Joost le

doi:10.1007/978-3-030-11135-9_2

Cited by 7 publications

(8 citation statements)

References 187 publications

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“…Specifically, brain disorders, which include a broad range of different conditions, suchas neurodegenerative diseases, demyelinating and neuroinflammatory diseases, tumors, dementias, infections or mental disorders, are a major public health problem, representing an important economic and social burden [ 4 ]. The brain is considered to be the most complex organ of the body, but its inaccessibility hinders the study of pathological processes [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Description of a CSF-Enriched miRNA Panel for the Study of Neurological Diseases

Martín

Gómez

Miguela

et al. 2021

Life

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Background: The study of circulating miRNAs in CSF has gained tremendous attention during the last years, as these molecules might be promising candidates to be used as biomarkers and provide new insights into the disease pathology of neurological disorders. Objective: The main aim of this study was to describe an OpenArray panel of CSF-enriched miRNAs to offer a suitable tool to identify and characterize new molecular signatures in different neurological diseases. Methods: Two hundred and fifteen human miRNAs were selected to be included in the panel, and their expression and abundance in CSF samples were analyzed. In addition, their stability was studied in order to propose suitable endogenous controls for CSF miRNA studies. Results: miR-143-3p and miR-23a-3p were detected in all CSF samples, while another 80 miRNAs were detected in at least 70% of samples. miR-770-5p was the most abundant miRNA in CSF, presenting the lowest mean Cq value. In addition, miR-26b-5p, miR-335-5p and miR-92b-3p were the most stable miRNAs and could be suitable endogenous normalizers for CSF miRNA studies. Conclusions: These OpenArray plates might be a suitable and efficient tool to identify and characterize new molecular signatures in different neurological diseases and would improve the yield of miRNA detection in CSF.

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Section: Introductionmentioning

confidence: 99%

Description of a CSF-Enriched miRNA Panel for the Study of Neurological Diseases

Martín

Gómez

Miguela

et al. 2021

Life

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“…The next stage of our study aimed to analyze changes in the adaptive capacity of the astrocytic network in response to modeling ischemia-like conditions. Hypoxia is one of the most common experimental models of brain cell damage [ 18 ]. Several studies have shown a significant decrease in the viability and functional activity of brain cells in models of 10-min acute hypoxia in vitro [ 18 , 19 , 20 ].…”

Section: Resultsmentioning

confidence: 99%

“…Hypoxia is one of the most common experimental models of brain cell damage [ 18 ]. Several studies have shown a significant decrease in the viability and functional activity of brain cells in models of 10-min acute hypoxia in vitro [ 18 , 19 , 20 ]. However, astrocytes are considered more resistant to hypoxic damage than neurons.…”

Section: Resultsmentioning

confidence: 99%

Signatures of the Consolidated Response of Astrocytes to Ischemic Factors In Vitro

Митрошина

Krivonosov

Burmistrov

et al. 2020

IJMS

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Whether and under what conditions astrocytes can mount a collective network response has recently become one of the central questions in neurobiology. Here, we address this problem, investigating astrocytic reactions to different biochemical stimuli and ischemic-like conditions in vitro. Identifying an emergent astrocytic network is based on a novel mathematical approach that extracts calcium activity from time-lapse fluorescence imaging and estimates the connectivity of astrocytes. The developed algorithm represents the astrocytic network as an oriented graph in which the nodes correspond to separate astrocytes, and the edges indicate high dynamical correlations between astrocytic events. We demonstrate that ischemic-like conditions decrease network connectivity in primary cultures in vitro, although calcium events persist. Importantly, we found that stimulation under normal conditions with 10 µM ATP increases the number of long-range connections and the degree of corresponding correlations in calcium activity, apart from the frequency of calcium events. This result indicates that astrocytes can form a large functional network in response to certain stimuli. In the post-ischemic interval, the response to ATP stimulation is not manifested, which suggests a deep lesion in functional astrocytic networks. The blockade of Connexin 43 during ischemic modeling preserves the connectivity of astrocytes in the post-hypoxic period.

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“…Native tissues additionally have different regions with differing physicochemical, biological, and topological features, that are essential for the wider system and consequentially in the corresponding artificial tissue models created to mimic specific behaviors (Figure 1). [39,40] As tissue function is inherently linked to tissue architecture, be it ducts, tubes or complex organizations, it is essential to replicate this native architecture to best achieve tissue specific functions. [41] For instance, in liver, architectural organization is believed to be essential to hepatic function.…”

Section: Mimicking Biology In Vitromentioning

confidence: 99%

Keeping It Organized: Multicompartment Constructs to Mimic Tissue Heterogeneity

Sanchez‐Rubio

Jayawarna

Maxwell

et al. 2023

Adv Healthcare Materials

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Tissue engineering aims at replicating tissues and organs to develop applications in vivo and in vitro. In vivo, by engineering artificial constructs using functional materials and cells to provide both physiological form and function. In vitro, by engineering three‐dimensional (3D) models to support drug discovery and enable understanding of fundamental biology. 3D culture constructs mimic cell–cell and cell–matrix interactions and use biomaterials seeking to increase the resemblance of engineered tissues with its in vivo homologues. Native tissues, however, include complex architectures, with compartmentalized regions of different properties containing different types of cells that can be captured by multicompartment constructs. Recent advances in fabrication technologies, such as micropatterning, microfluidics or 3D bioprinting, have enabled compartmentalized structures with defined compositions and properties that are essential in creating 3D cell‐laden multiphasic complex architectures. This review focuses on advances in engineered multicompartment constructs that mimic tissue heterogeneity. It includes multiphasic 3D implantable scaffolds and in vitro models, including systems that incorporate different regions emulating in vivo tissues, highlighting the emergence and relevance of 3D bioprinting in the future of biological research and medicine.

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In Vitro Models of Brain Disorders

Cited by 7 publications

References 187 publications

Description of a CSF-Enriched miRNA Panel for the Study of Neurological Diseases

Description of a CSF-Enriched miRNA Panel for the Study of Neurological Diseases

Signatures of the Consolidated Response of Astrocytes to Ischemic Factors In Vitro

Keeping It Organized: Multicompartment Constructs to Mimic Tissue Heterogeneity

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