In vitro models of oxidative stress in rat erythrocytes: Effect of antioxidant supplements

Reddy, Chada S.; Subramanyam, M. V. V.; Vani, R.; Devi, S. Asha

doi:10.1016/j.tiv.2007.06.010

Cited by 86 publications

(38 citation statements)

References 28 publications

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“…GSH and CAT are major enzymes that control the biological effect of ROS [42]. While GSH quenches the free radicals by serving as electron donor, CAT converts hydrogen peroxide to water and oxygen [43]. Our data show, incubation with AgNPs results in significant decrease in GSH concentration and CAT activity compared to control.…”

Section: Discussionmentioning

confidence: 62%

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

Ferdous

Beegam

Tariq

et al. 2018

Cell Physiol Biochem

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Background/Aims: Silver nanoparticles (AgNPs) are increasingly used as antimicrobial agents and drug carriers in various biomedical fields. AgNPs can encounter erythrocytes either directly following intravenous injection, or indirectly via translocation from the site of administration. However, information regarding the pathophysiological effects and possible mechanism of action of AgNPs on the erythrocytes are still inadequately studied. Thus, the aim of our study was to investigate the mechanism underlying the effect of coating and concentration of AgNPs on mouse erythrocytes in vitro. Methods: We studied the interaction of polyvinylpyrrolidone (PVP) and citrate (CT) coated AgNPs (10 nm) at various concentrations (2.5, 10, 40 µg/ml) with mouse erythrocytes in vitro using various techniques including transmission electron microscopy (TEM), hemolysis, and colorimetric measurement of markers of oxidative stress comprising malondialdehyde (MDA), reduced glutathione (GSH), and catalase (CAT). Intracellular calcium (Ca²⁺) was determined using Fura 2AM fluorescence. Annexin V was quantified using ELISA and the caspase 3 was determined both flurometrically and by western blot technique. Results: Following incubation of the erythrocytes with AgNPs, both PVP- and CT- AgNPs induced significant and dose - dependent increase in hemolysis. TEM revealed that both PVP- and CT- AgNPs were taken up by erythrocytes. The erythrocyte susceptibility to lipid peroxidation measured by MDA was significantly increased in both PVP-and CT- AgNPs. The concentration of GSH and CAT activity were significantly decreased by both types of AgNPs. Additionally, PVP- and CT- AgNPs significantly increased intracellular Ca²⁺ in a dose -dependent manner. Likewise, the concentration of the cellular protein annexin V was significantly and dose - dependently enhanced by both types of AgNPs. Furthermore, PVP- and CT- AgNPs induced significant increase in calpain activity in incubated erythrocytes. Conclusion: We conclude that both PVP- and CT- AgNPs causes hemolysis, and are taken up by erythrocytes. Moreover, we demonstrated that AgNPs induces oxidative stress and eryptosis. These findings provide evidence for the potential pathophysiological effect of PVP-and CT- AgNPs on erythrocyte physiology.

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Section: Discussionmentioning

confidence: 62%

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

Ferdous

Beegam

Tariq

et al. 2018

Cell Physiol Biochem

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“…Our data corroborate the hemolysis findings; erythrocytes incubated with SiNP exhibited a significant and dose-dependent increase in MDA. This suggests that SiNP induced oxidative stress to the erythrocyte; the plasma membrane is often the initial site of damage, and the resulting peroxidation of membrane lipids causes hemolysis and cross-links between protein and lipid molecules to different extents [35]. We have recently reported that incubation of erythrocytes with DEP (1-100 µg/ml) caused a significant and dose-dependent increase in MDA in erythrocytes of rats, but not humans or mice [12].…”

Section: Discussionmentioning

confidence: 99%

“…Erythrocytes contain intracellular antioxidant enzymes such as SOD, catalase and GSH, that work together to prevent oxidative damage to the cell [35]. SOD catalyzes the conversion of superoxide radicals to hydrogen peroxide, while catalase converts hydrogen peroxide into water [35].…”

Section: Discussionmentioning

confidence: 99%

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Interaction of Amorphous Silica Nanoparticles with Erythrocytesin Vitro: Role of Oxidative Stress

Nemmar¹,

Beegam²,

Yuvaraju³

et al. 2014

Cell Physiol Biochem

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Background/Aims: The use of engineered nanomaterials in the form of nanoparticles (NP) for various biomedical applications, as well as in consumer products, has raised concerns about their safety for human health. These NP are intended to be administered directly into the circulation following intravenous injection, or they may reach the circulation following other routes of administration such as oral or inhalation, and interact with circulating cells such as erythrocytes. However, little is known about the interaction of amorphous SiNP with erythrocytes. Methods: We studied the interaction of amorphous silica nanoparticles (SiNP) at various concentrations (1, 5, 25 and 125µg/ml) with mouse erythrocytes in vitro. Results: Incubation of erythrocytes with SiNP caused a dose-dependent hemolytic effect. Likewise, the activity of lactate dehydrogenase was dose-dependently increased by SiNP. Transmission electron microscopy analysis revealed that SiNP are taken up by erythrocytes. Lipid erythrocyte susceptibility to in vitro peroxidation measured by malondialdehyde showed a significant and dose-dependent increase in erythrocytes. SiNP also enhanced the antioxidant activities of superoxide dismutase (SOD), catalase and reduced glutathione (GSH). Moreover, SiNP increased caspase 3, triggered annexin V-binding and caused a dose-dependent increase of cytosolic calcium concentration. Conclusion: It can be concluded that SiNP cause a dose-dependent hemolytic activity and are taken up by the erythrocytes. We also found that SiNP induce the occurrence of oxidative activity, apoptosis and increase cytosolic Ca²⁺, which may explain their haemolytic activity. Our in vitro data suggest that SiNP may, plausibly, lead to anemia and circulatory disorders in vivo.

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“…This could be responsible for the cationic flow disturbances and would result in the rigidity changes of the membrane. An earlier study by Shiva Shankar Reddy et al(2007) showed that the interaction between aluminum on Na-K-ATP ase and Ca-ATP ase activities could lead to greater membrane rigidity and changes in osmotic fragility test.…”

Section: Discussionmentioning

confidence: 99%

Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Taib

Budin

Ain

et al. 2009

J. Zhejiang Univ. Sci. B

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Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, chemopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat's red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.

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In vitro models of oxidative stress in rat erythrocytes: Effect of antioxidant supplements

Cited by 86 publications

References 28 publications

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

Interaction of Amorphous Silica Nanoparticles with Erythrocytesin Vitro: Role of Oxidative Stress

Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

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