In Vitro Mouse Ovarian Follicle Growth and Maturation in Alginate Hydrogel: Current State of the Art

Filatov, Maxim A.; Khramova, Yu. V.; Semenova, M. L.

doi:10.32607/20758251-2015-7-2-48-56

Cited by 25 publications

(20 citation statements)

References 43 publications

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“…For example, laminin and type IV collagen have been observed in the basal lamina of follicles in several different species [ 13 – 16 ], suggesting addition of ECM proteins to 2-D follicle cultures may help mimic normal environmental signaling that occurs in vivo to promote follicle growth in 2-D culture systems. However, it has been recognized that 2-D follicle culture is not sufficient to sustain the normal architecture of follicles in vitro [ 1 , 4 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, further technical advancement has emerged in the form of 3-D follicle culture, which better sustains normal follicle structure in vitro. In 3-D follicle culture systems, isolated follicles are individually encapsulated in various types of substrates such as collagen, alginate, hyaluronan hydrogel, PEG, or Matrigel to maintain the normal 3-D architecture of the follicle in vitro [ 1 , 4 , 17 ]. To date, encapsulation with alginate gel has been the most widely applied system for 3-D follicle culture [ 4 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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A Simplified Method for Three-Dimensional (3-D) Ovarian Tissue Culture Yielding Oocytes Competent to Produce Full-Term Offspring in Mice

et al. 2015

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In vitro growth of follicles is a promising technology to generate large quantities of competent oocytes from immature follicles and could expand the potential of assisted reproductive technologies (ART). Isolated follicle culture is currently the primary method used to develop and mature follicles in vitro. However, this procedure typically requires complicated, time-consuming procedures, as well as destruction of the normal ovarian microenvironment. Here we describe a simplified 3-D ovarian culture system that can be used to mature multilayered secondary follicles into antral follicles, generating developmentally competent oocytes in vitro. Ovaries recovered from mice at 14 days of age were cut into 8 pieces and placed onto a thick Matrigel drop (3-D culture) for 10 days of culture. As a control, ovarian pieces were cultured on a membrane filter without any Matrigel drop (Membrane culture). We also evaluated the effect of activin A treatment on follicle growth within the ovarian pieces with or without Matrigel support. Thus we tested four different culture conditions: C (Membrane/activin-), A (Membrane/activin+), M (Matrigel/activin-), and M+A (Matrigel/activin+). We found that the cultured follicles and oocytes steadily increased in size regardless of the culture condition used. However, antral cavity formation occurred only in the follicles grown in the 3-D culture system (M, M+A). Following ovarian tissue culture, full-grown GV oocytes were isolated from the larger follicles to evaluate their developmental competence by subjecting them to in vitro maturation (IVM) and in vitro fertilization (IVF). Maturation and fertilization rates were higher using oocytes grown in 3-D culture (M, M+A) than with those grown in membrane culture (C, A). In particular, activin A treatment further improved 3-D culture (M+A) success. Following IVF, two-cell embryos were transferred to recipients to generate full-term offspring. In summary, this simple and easy 3-D ovarian culture system using a Matrigel drop and activin A supplementation (M+A) provides optimal and convenient conditions to support growth of developmentally competent oocytes in vitro.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Simplified Method for Three-Dimensional (3-D) Ovarian Tissue Culture Yielding Oocytes Competent to Produce Full-Term Offspring in Mice

et al. 2015

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“…However, technologies of ovarian tissue in vitro culture are being actively developed (Roy & Treacy, 1993;Abir et al, 1997Abir et al, , 1999Abir et al, , 2001Nayudu et al, 2003;Xu et al, 2011b). The main aim of these technologies is to produce mature oocytes capable of fertilization from pieces of in vitro cultured ovarian tissue (Filatov et al, 2015). Embryos obtained after the fertilization of oocytes may be cryopreserved and transferred into the patient's uterus after full recovery.…”

Section: Main Strategies Of Female Fertility Preservationmentioning

confidence: 99%

Female fertility preservation strategies: cryopreservation and ovarian tissuein vitroculture, current state of the art and future perspectives

Filatov

Khramova

Kiseleva

et al. 2016

Zygote

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In the present review, the main strategies of female fertility preservation are covered. Procedures of fertility preservation are necessary for women who suffer from diseases whose treatment requires the use of aggressive therapies, such as chemotherapy and radiotherapy. These kinds of therapy negatively influence the health of gametes and their progenitors. The most commonly used method of female fertility preservation is ovarian tissue cryopreservation, followed by the retransplantation of thawed tissue. Another approach to female fertility preservation that has been actively developed lately is the ovarian tissue in vitro culture. The principal methods, advantages and drawbacks of these two strategies are discussed in this article.

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“…Alginate hydrogels have been used for encapsulation to provide support for culture and maturation of mouse follicles (Filatov et al, 2014; Hornick et al, 2012; Hornick et al, 2013; Telfer and Zelinski, 2013; Xiao et al, 2015a; Xu et al, 2009) and human follicles (Laronda et al, 2014; Xiao et al, 2015b) in vitro . Oocytes can be retrieved from alginate-encapsulated murine follicles for in vitro fertilization and subsequently implanted to produce healthy offspring (Xu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…In this report, we investigated a strategy for transplantation of ovarian follicles within nondegradable alginate hydrogels to allow in vivo follicle maturation, with subsequent retrieval of the graft to obtain mature oocytes for in vitro fertilization (IVF). Alginate hydrogels have been used for encapsulation to provide support for culture and maturation of mouse follicles (Filatov, Khramova, & Semenova, 2014;Hornick, Duncan, Shea, & Woodruff, 2012;Hornick, Duncan, Shea, & Woodruff, 2013;Telfer & Zelinski, 2013;Xiao, Duncan et al, 2015;Xu, Banc, Woodruff, & Shea, 2009) and human follicles (Laronda et al, 2014;Xiao, Zhang et al, 2015) in vitro. Oocytes can be retrieved from alginate-encapsulated murine follicles for IVF and subsequently implanted to produce healthy offspring (Xu, Kreeger, Shea, & Woodruff, 2006).…”

mentioning

confidence: 99%

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Rios

Kniazeva

Lee

et al. 2018

Biotech & Bioengineering

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Cancer survivorship rates have drastically increased due to improved efficacy of oncologic treatments. Consequently, clinical concerns have shifted from solely focusing on survival to quality of life, with fertility preservation as an important consideration. Among fertility preservation strategies for female patients, ovarian tissue cryopreservation and subsequent reimplantation has been the only clinical option available to cancer survivors with cryopreserved tissue. However, follicle atresia after transplantation and risk of reintroducing malignant cells have prevented this procedure from becoming widely adopted in clinics. Herein, we investigated the encapsulation of ovarian follicles in alginate hydrogels that isolate the graft from the host, yet allows for maturation after transplantation at a heterotopic (i.e., subcutaneous) site, a process we termed in vivo follicle maturation. Survival of multiple follicle populations was confirmed via histology, with the notable development of the antral follicles. Collected oocytes (63%) exhibited polar body extrusion and were fertilized by intracytoplasmic sperm injection and standard in vitro fertilization procedures. Successfully fertilized oocytes developed to the pronucleus (14%), two-cell (36%), and four-cell (7%) stages. Furthermore, ovarian follicles cotransplanted with metastatic breast cancer cells within the hydrogels allowed for retrieval of the follicles, and no mice developed tumors after removal of the implant, confirming that the hydrogel prevented seeding of disease within the host. Collectively, these findings demonstrate a viable option for safe use of potentially cancer-laden ovarian donor tissue for in vivo follicle maturation within a retrievable hydrogel and subsequent oocyte collection. Ultimately, this technology may provide novel options to preserve fertility for young female patients with cancer.

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In Vitro Mouse Ovarian Follicle Growth and Maturation in Alginate Hydrogel: Current State of the Art

Cited by 25 publications

References 43 publications

A Simplified Method for Three-Dimensional (3-D) Ovarian Tissue Culture Yielding Oocytes Competent to Produce Full-Term Offspring in Mice

A Simplified Method for Three-Dimensional (3-D) Ovarian Tissue Culture Yielding Oocytes Competent to Produce Full-Term Offspring in Mice

Female fertility preservation strategies: cryopreservation and ovarian tissuein vitroculture, current state of the art and future perspectives

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

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