In vitro Multiple Shoots Formation in Wild Lettuce (Launaea taraxacifolia) (Willd.) Amin ex C. Jeffrey

Obembe, Olawole O.; Bello, Oluwakemi A.; Aworunse, Oluwadurotimi S.; Popoola, Jacob O.; Akposibruke, Olivia; Olukanmi, Babafemi; Mary, Omwamba

doi:10.9734/arrb/2017/36212

Cited by 4 publications

(6 citation statements)

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“…T13 had the best outcome (100 % clean culture), T14 had the secondbest result (77 %), and T16 had the lowest percentage clean culture (40 %). It was also observed that pretreatment of Launaea taraxacifolia with 70% ethanol for 5 minutes, followed by step-wise double disinfection in 10% and 15% sodium hypochlorite, yielded good results (Obembe et al, 2017).…”

Section: Resultsmentioning

confidence: 94%

Surface Sterilization Protocol for Nodal Cultures of Potato (Solanum tuberosum L.)

2021

JBAH

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The process of surface sterilizing plant materials is a difficult step in constructing a plant tissue culture technique. Nodal explants from potted plants of Solanum tuberosum L. grown in the screenhouse were given various surface sterilization procedures before being introduced into sterile Murashige and Skoog (MS) culture. A total of 16 treatments were performed with varied periods of immersion in two sterilant successions: 70% (v/v) ethanol (20 s, 1 min, 3 min, and 5 min) and 10% (w/v) calcium hypochlorite (Ca(ClO)2) granules (5, 10, 15, and 20 min) at varying times, followed by three sterile distilled water rinses. After 4 weeks in culture, 70 % ethanol (20 s)+10 % Ca(ClO)2 (15 min) and 70 % ethanol (3 min)+10 % Ca(ClO)2 (20 min) treatments yielded 100 % clean culture. 70 % ethanol (20 seconds) Plus 10% calcium (ClO) 2 (15 min) is recommended as the optimum surface sterilizing protocol since it produced 90% growth compared to 60% growth in 70% ethanol (3 min) + 10% Ca(ClO)2 (20 min). This is a preliminary study, and more research is needed to develop an efficient, successful, and repeatable methodology for surface sterilization of various explants utilized for tissue culture of S. biafrae acquired from the open field.

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Section: Resultsmentioning

confidence: 94%

Surface Sterilization Protocol for Nodal Cultures of Potato (Solanum tuberosum L.)

2021

JBAH

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“…These reports showed that genotype, explant age, plant growth hormone concentrations, the amount of micro-and macronutrients, and the carbon source all play a vital role in regeneration. Callus and shoot induction using various explants have been performed in wild and cultivated lettuce (Park and Lim 1997;Mohebodini et al 2011;Ahsan et al 2015;Armas et al 2017;Obembe et al 2017) however, our study is the first to achieve the in vitro propagation of Lactuca indica.…”

Section: Discussionmentioning

confidence: 94%

“…Increasing demand, unregulated harvesting, and the lack of a proper strategy for replenishing medicinal plants such as Lactuca indica have led to a decline in these natural resources in their natural habitat (Anis and Ahmad 2016). In nature, Lactuca indica propagates through seed production, but as with other lettuces, the seed has low viability and dormancy problems when the light requirement for seed germination is offset by low temperature (Eenink 1977;Sakpere et al 2011;Obembe et al 2017). Vegetative propagation is also possible, but limited.…”

Section: Introductionmentioning

confidence: 99%

“…Also, this method provides a continuous supply of potential resources without any seasonal and environmental limitations, which ensures their practical application on a commercial scale (Kumari et al 2016). Most of the in vitro regeneration research in lettuce has focused on the cultivated lettuce (Lactuca sativa) (Koevaxy et al 1978;Alconero 1983;Webb et al 1984;Pink 1992;Teng et al 1992;Ampomah-Dwamena et al 1997;Park and Lim 1997;Pink and Carter 1987;Mohebodini et al 2011;Latif et al 2014;Ahsan et al 2015;Armas et al 2017), with very few focusing on wild lettuce (Koevaxy et al, 1978;Obembe et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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In vitro propagation, lactucin quantification, and antibacterial activity of Indian lettuce (Lactuca indica L.)

Oliya

Kim

Lee

2021

In Vitro Cell.Dev.Biol.-Plant

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Lactuca indica L. (Asteraceae), a wild lettuce, is used as a vegetable and in traditional medicine. This study aims to establish in vitro propagation protocol and evaluate lactucin and antibacterial property from in vitro and natural plant tissues. Leaf blades and petioles were cultured in vitro on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) and 1.2 mg L–1 indoleacetic acid (IAA). Leaf petioles and a lower BAP concentration (0.5 mg L–1) were optimal for direct shoot induction, while the leaf blade and a higher BAP (4 mg L–1) concentration performed best for callus induction. When the callus was subcultured, 98.7% of samples regenerated plants on MS medium supplemented with 1.5 mg L–1 BAP and 0.5 mg L–1 IAA. MS medium containing 1 mg L–1 IAA was best for in vitro rooting. A high-performance liquid chromatography analysis of the in vitro samples revealed a higher amount of lactucin (sesquiterpene lactones) in the root than the callus and the leaf, whereas in naturally grown plants, higher lactucin amounts were obtained from the juvenile root followed by the root of the flowering plant and juvenile leaf as the lowest concentration. All tissue extracts showed antibacterial activity against Pseudomonas fuscovaginae (a rice pathogen) and Escherichia coli, which was directly proportional to amount of lactucin produced. This in vitro regeneration and phytochemical investigation will facilitate the further exploitation of this useful wild plant.

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“…It is the only species in the Asteraceae family without any trichome. The leaf, stem, and nodes are potential explants for regeneration of Launaea taraxacifolia [ 6 – 10 ]. A picture of the leaves with flowers is shown in Figure 2 .…”

Section: Resultsmentioning

confidence: 99%

The Ethnopharmacological and Nutraceutical Relevance of Launaea taraxacifolia (Willd.) Amin ex C. Jeffrey

Adinortey

Sarfo

Kwarteng

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Launaea taraxacifolia (Willd.) Amin ex C. Jeffrey is a herb found mostly in tropical Africa. The plant, commonly found in West Africa, is used in the management of many diseases including cardiovascular, respiratory, haematological, endocrine, and metabolic diseases in Ghana, Nigeria, Benin, Serra Leone, and Senegal. This piece provides comprehensive and updated information on the traditional uses, phytochemical constituents, and pharmacological and toxicological information available on Launaea taraxacifolia to support its medicinal uses and also unearth knowledge gaps for future studies. An electronic literature search using search engines, namely, Google Scholar, ScienceDirect, and PubMed, was carried out to obtain information on the plant. Both common and scientific names of the plant were used as keywords for the search process. This paper captured information on Launaea taraxacifolia from 1985 to 2018. The search revealed that the leaves of the plant possess nutritional/pharmacological effects on diseases such as diabetes mellitus, hypertension, cancer, malaria, bacterial infections, and arthritis. The leaf has been shown to be a rich source of phytoconstituents such as flavonoids, phenolic acids, tannins, alkaloids, glycosides, coumarins, triterpenoids, ascorbic acid, lycopene, and β-carotene. Also, isolated phytoconstituents as well as the safety profile of the plant have been documented. This review on Launaea taraxacifolia has provided a one-stop documentation of information in support of the several purported ethnopharmacological uses of the plant. It also reveals information gaps such as the need to research into its pharmacokinetics, interactions with drugs of importance, and its development into a plant-based drug in order to expand its clinical use.

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In vitro Multiple Shoots Formation in Wild Lettuce (Launaea taraxacifolia) (Willd.) Amin ex C. Jeffrey

Cited by 4 publications

References 0 publications

Surface Sterilization Protocol for Nodal Cultures of Potato (Solanum tuberosum L.)

Surface Sterilization Protocol for Nodal Cultures of Potato (Solanum tuberosum L.)

In vitro propagation, lactucin quantification, and antibacterial activity of Indian lettuce (Lactuca indica L.)

The Ethnopharmacological and Nutraceutical Relevance of Launaea taraxacifolia (Willd.) Amin ex C. Jeffrey

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