In vitro multiplication, genetic fidelity and phytochemical potentials of Vaccinium arctostaphylos L.: An endangered medicinal plant

Bakhshipour, Mehdi; Mafakheri, Mohammad; Kordrostami, Mojtaba; Zakir, Ali; Rahimi, Najmeh; Feizi, Fatemeh; Mohseni, Mohsen

doi:10.1016/j.indcrop.2019.111812

Cited by 20 publications

(7 citation statements)

References 71 publications

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“…The current study results are in confirmation with the findings of Rao et al [ 31 ] that zeatin treatment produced a significantly higher number of shoots of Alpinia galanga (Zingiberaceae) compared with BAP, TDZ, and kinetin treatments. The significance of zeatin compared with BAP, kinetin, and TDZ has also been reported for shoot multiplication of several other herbaceous and non-herbaceous species [ 31 , 32 , 33 , 34 ]. Besides, zeatin was also found to be more effective for microrhizome induction of ‘Bentong’ ginger, but it was less effective for shoot multiplication compared with BAP [ 35 ].…”

Section: Discussionmentioning

confidence: 94%

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

Zahid

Jaafar

Hakiman

2021

Plants

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‘Bentong’ ginger is the most popular variety of Zingiber officinale in Malaysia. It is vegetatively propagated and requires a high proportion of rhizomes as starting planting materials. Besides, ginger vegetative propagation using its rhizomes is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied in many plant species to produce their disease-free planting materials. As ‘Bentong’ ginger is less known for its micropropagation, this study was conducted to investigate the effects of Clorox (5.25% sodium hypochlorite (NaOCl)) on explant surface sterilization, effects of plant growth regulators, and basal media on shoots’ multiplication and rooting. The secondary metabolites and antioxidant activities of the micropropagated plants were evaluated in comparison with conventionally propagated plants. Rhizome sprouted buds were effectively sterilized in 70% Clorox for 30 min by obtaining 75% contamination-free explants. Murashige and Skoog (MS) supplemented with 10 µM of zeatin was the suitable medium for shoot multiplication, which resulted in the highest number of shoots per explant (4.28). MS medium supplemented with 7.5 µM 1-naphthaleneacetic acid (NAA) resulted in the highest number of roots per plantlet. The in vitro-rooted plantlets were successfully acclimatized with a 95% survival rate in the ex vitro conditions. The phytochemical analysis showed that total phenolic acid and total flavonoid content and antioxidant activities of the micropropagated plants were not significantly different from the conventionally propagated plants of ‘Bentong’ ginger. In conclusion, the present study’s outcome can be adopted for large-scale propagation of disease-free planting materials of ‘Bentong’ ginger.

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Section: Discussionmentioning

confidence: 94%

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

Zahid

Jaafar

Hakiman

2021

Plants

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“…In all tests, methanolic extract of the leaves of the mother plant showed less activity compared to directly regenerated and callus-mediated regenerated plants. Similarly, addition of PGRs enhances the production of several bioactives including phenolics, flavonoids, and concomitant enhanced antioxidant activity in several medicinal plants like Tulbaghia violacea, Scutellaria altissima, and Vaccinium arctostaphylos (Ncube et al 2011;Grzegorczyk-Karolak et al 2015;Bakhshipour et al 2019). Conversely, addition of PGRs under in vitro condition results in reduced accumulation of bioactives in Swertia corymbosa (Mahendran and Bai 2014), resulting in variation of antioxidant properties between in vitro and conventionally grown plants (Aremu et al 2012) indicating species-specific physiological response.…”

Section: Total Phenolics Flavonoids and Antioxidant Potentialmentioning

confidence: 99%

In vitro regeneration, antioxidant potential, and genetic fidelity analysis of Asystasia gangetica (L.) T.Anderson

Dilkalal

Akula

Umesh

2021

In Vitro Cell.Dev.Biol.-Plant

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An effective protocol for the plant regeneration via direct and indirect organogenesis has been developed from leaf explants of Asystasia gangetica (L.), cultured on Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of auxin and cytokinins. Approximately 86% of explants produced direct shoots on MS medium containing 0.5 mg L −1 6-benzyladenine (BA) and 10 μg L −1 Triacontanol (TRIA) with a maximum of 4.82 ± 0.29 shoots per leaf segment. For production of callus-mediated plantlets (indirect), primarily callus was induced on MS medium containing 2 mg L −1 2,4dichlorophenoxyacetic acid (2,4-D), which was then subcultured on medium with 0.1 mg L −1 naphthaleneacetic acid (NAA), 0.5 mg L −1 BA, and 1 to 8 mg L −1 2-isopentenyl adenine (2iP) in order to develop organogenic callus and subsequent shoot induction. A maximum of 6.84 ± 0.05 shoots per callus clump was obtained on MS media supplemented with 4 mg L −1 2iP, 0.5 mg L −1 BA, and 0.1 mg L −1 NAA. The shootlets produced roots when cultured on half-strength MS media supplemented with 2 mg L −1 indole-3-butyric acid (IBA). In vitro propagated plantlets were hardened on soil rite and acclimatized to field condition with 85% survivability. The chlorophyll content of acclimatized plants was comparable with that of the mother plant, while stomatal micromorphology of regenerated plants exhibited no abnormalities. The radical scavenging and antioxidant activity of methanolic extract of leaves were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP), and phosphomolybdenum test. In all experiments, regenerated plants exhibited enhanced antioxidant potential indicating micropropagated plants could be exploited for isolation of novel biomolecules. Further, the genetic homogeneity of acclimatized plants was confirmed by PCR-based start codon targeted (SCoT) markers and ycf1b DNA barcoding primers which exhibited monomorphic bands identical to the normal mother plant and no variations were observed.

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“…Plant metabolites can be isolated from naturally grown plants, however, due to environmental limits, as seasonal restrictions, particular soil selection or growth conditions, the commercial production is limited [18]. Traditional methods take a long time since the plant needs several years to mature and attain the necessary level of metabolite production.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Vaccinium corymbosum L.: An Alternative Procedure for the Production of Secondary Metabolites

et al. 2022

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In vitro culture has become a dependable approach for the mass production of plant material as the market for innovative plant-derived medicinal approaches has grown significantly. Furthermore, because it permits manipulation of biosynthetic routes to boost the production and accumulation of certain compounds, this technology has enormous potential for the manufacture of natural bioactive chemicals. As a result, the goal of this study was to develop an efficient micropropagation system for biomass production and to investigate the accumulation of bioactive compounds from Vaccinium corymbosum L., Duke and Hortblue Petite cultivars. Two in vitro plant tissue culture systems were used for shoots production: a solid medium (5 g/L Plant agar) and liquid medium (Plantform bioreactor). The culture medium used was Woddy Plant Medium (WPM) supplemented with two growth regulators: 0.5 mg/L and 1 mg/L zeatina (Z) and 5 mg/L N6-(2-Isopentenyl) adenine (2iP). The content of phenolic compounds, carotenoids, and chlorophylls of the in vitro shoot extracts were examined via the HPLC-DAD-MS/MS technique. The results showed that cv. Hortblue Petite produced a higher amount of biomass compared with cv. Duke, on all variants of culture media in both systems (solid and liquid), while the shoots extract of the Duke variety in the liquid culture system (under all concentrations of growth regulators) had the highest content of total phenolic compounds (16,665.61 ± 424.93 μg/g). In the case of the lipophilic compounds analysed (chlorophylls and carotenoids), the solid medium reported the highest values, whereas media supplemented with 0.5 mg/L Z was proved to have the richest total content for both cultivars.

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In vitro multiplication, genetic fidelity and phytochemical potentials of Vaccinium arctostaphylos L.: An endangered medicinal plant

Cited by 20 publications

References 71 publications

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

In vitro regeneration, antioxidant potential, and genetic fidelity analysis of Asystasia gangetica (L.) T.Anderson

Micropropagation of Vaccinium corymbosum L.: An Alternative Procedure for the Production of Secondary Metabolites

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