In vitro multiplication of Mentha piperita L. and comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method

Radomir, Ana-Maria; Stan, Ramona; Pandelea, Mariana Letiția; Vizitiu, Diana Elena

doi:10.24326/asphc.2022.4.5

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…Similar observations were reported in a study on tobacco [Cantrill et al 2001]. In vitro cultures are the primary tool for biotechnologists' work in the callus multiplication process to create new varieties and interspecies hybrids and obtain valuable metabolites and biopharmaceuticals [Radomir et al 2022]. Bidabadi and Jain [2020] point out that many events during the plant regeneration process can be controlled by manipulating the signaling pathways associated with phytohormone interaction, explant injury, and programmed cell death.…”

Section: Discussionsupporting

confidence: 64%

Regenerative potential and its variability in different topophysical zones of Kalanchoe daigremontiana leaves in in vitro culture conditions

Winiarczyk,

Czerska,

Denisow

et al. 2024

Acta Sci. Pol. Hortorum Cultus

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Plants of the genus Kalanchoe are the most frequently purchased potted plants because of their decorative qualities and easy cultivation. Modern commercial plants have unique shapes that are usually not genetically determined; hence, in vitro cultures are used to propagate such specimens. The efficiency of regeneration is significantly affected by the type of explant used when that explant was previously located on one of several parts of a complex plant organ. The study aimed to investigate the influence of the topophysical location of collected explants on the regeneration efficiency of adventitious shoots. Various regeneration pathways were observed in the in vitro micropropagation of Kalanchoe daigremontiana leaf fragments, i.e., direct and indirect organogenesis from the callus stage. The results show a dependent relationship between the location of the collected explant and its regenerative potential. The most remarkable regenerative capacity of K. daigremontiana was found in leaf blade fragments taken from the middle and proximal zones. In addition, the explants collected from the marginal zone of the leaf blade were notably more valuable than those from the part located near the midrib. The research results indicate that the growth and development of new seedlings propagated vegetatively with the in vitro method depends on their location on the mother plant.

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Section: Discussionsupporting

confidence: 64%

Regenerative potential and its variability in different topophysical zones of Kalanchoe daigremontiana leaves in in vitro culture conditions

Winiarczyk,

Czerska,

Denisow

et al. 2024

Acta Sci. Pol. Hortorum Cultus

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Mentha

Akram,

Akhtar,

Akram

et al. 2023

Essentials of Medicinal and Aromatic Crops

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Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants

Duta-Cornescu

Constantin

Pojoga

et al. 2023

IJMS

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Cell and tissue plant cultures are used either to save vulnerable species from extinction or to multiply valuable genotypes, or both, and are widely applied for economically important plant species. For medicinal plants, the use of in vitro technologies for the production of secondary metabolites and pathogen-free plants has been greatly developed. Two opposite aspects characterize the in vitro micropropagation of medicinal plants: maintaining genetic fidelity for the perpetuation and preservation of elites, and the identification and exploitation of somaclonal variations associated with new, useful traits. A balance between what is advantageous and what is undesirable is necessary, and this implies the identification of somaclonal variability at all levels, from the phenotypic to molecular ones. This review addresses the somaclonal variation arising from the in vitro multiplication of medicinal plants from three perspectives: cytogenetics, genetics, and epigenetics. The possible causes of the appearance of somaclones, the methods for their identification, and the extent to which they are desirable are presented comparatively for different plant species with therapeutic properties. The emphasis is on the subtle changes at the genetic and epigenetic level, as it results from the application of methods based on DNA markers.

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In vitro multiplication of Mentha piperita L. and comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method

Cited by 3 publications

References 15 publications

Regenerative potential and its variability in different topophysical zones of Kalanchoe daigremontiana leaves in in vitro culture conditions

Regenerative potential and its variability in different topophysical zones of Kalanchoe daigremontiana leaves in in vitro culture conditions

Mentha

Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants

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