In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes Using Different Explants

Abstract

Stevia rebaudiana Bertoni that is a member of the Compositae family is one of the most valuable tropical medicinal plants. The origin of Stevia is South America, where it can be seen as a wild plant. Further it could be found in semi-arid habitat ranging from grassland to scrub forest to mountain terrain. Conventional cultivation or propagation methods of Stevia are as well known for time consuming, unpredictable, unreliable and less productive. Therefore, there is a crucial need to develop methods for rapid multiplication of this valuable plant. Plantlet produced through stem cutting has unstability, however the micropropagation of Stevia which may overcome some limitations associated with conventional method can be used for a rapid multiplication. Accordingly, the study results showed that different explants such as leaf, stem and root transferred to different tissue culture media for in vitro multiplication of two Stevia rebaudiana (Bertoni) genotypes could be reproduced by using different explants.

Exploring the Potential of Blueberry Extracts as Natural Antifungal and Bioherbicidal Agents in Agriculture: Composition and Antioxidant Activity

“…The V. myrtillus plant collected from the Eastern Black Sea Region in Turkey exhibited a total phenolic content of 1035 ± 16 mg gallic acid/100 g fw and a total avonoid content of 150 ± 4 mg quercetin equivalent/100 g fw (Altıok et al, 2022). Furthermore, the total phenolic content of V. myrtillus fruits and leaves collected from various locations in the Rize Province ranged from 184.2 to 556.1 mg GAE/100 g DW and from 69.9 to 212.9 mg GAE/100 g DW, respectively (Seyis et al, 2019). In Romania, the total phenolic content of V. myrtillus populations collected from different locations and years varied between 144.50 and 796.30 mg GAE/100 g FW, depending on the location and year (Ciulca et al, 2021).…”

Effect of paclobutrazol on growth and root morphology of 12 crossed stevia in Vitro

“…The growth regulators that play the most role in root induction are auxins, especially IAA (Indole-3-Acetic Acid) because they are most abundant in plant tissues (Zhao, 2010). In the research of Merindasya et al (2013) the best concentration of IAA in stevia tissue culture was 0.5 mg/L and in the study of Seyis et al (2017) 1 mg/L IAA was also used as a root induction medium for the treatment of the Stevia genotype.…”

Chromosome stability of in vitro propagated Cucurbita cultivars

“…Plant population produced by direct organogenesis from shoot meristem and leaf explants are homogenous. Therefore, genetically identical plants could be provided via regeneration in large scales (Seyis et al, 2017). Some factors such as Plant Growth Regulator (PGR) balance, culture conditions, genotype and explant type are important for successful plant regeneration.…”