In vitro mutagenesis of Chrysanthemum morifolium cultivars using ethylmethanesulphonate (EMS) and mutation assessment by ISSR and IRAP markers

Nasri, Fardin; Zakizadeh, Hedayat; Vafaee, Yavar; Mozafari, Ali Akbar

doi:10.1007/s11240-021-02163-7

Cited by 23 publications

(11 citation statements)

References 64 publications

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“…Colour of a ower is the primary feature with the highest appeal for consumers, even higher than ower scent (Nasri et al, 2021). In this study, four clones showed phenotypic variation in ower colour as compare to mother plant (Fig.…”

Section: Screening Of Somaclonesmentioning

confidence: 60%

Regeneration of chrysanthemum (Chrysanthemum morifolium) via somatic embryogenesis and screening of clones for agronomic traits

Ali

Raza

Shahzad

et al. 2023

Plant Cell Tiss Organ Cult

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Chrysanthemum morifolium propagation using conventional method of stem cutting produces weak plants and showed delayed anthesis above 20°C with reduced ower diameter. Therefore, in the present study chrysanthemum plantlets were produced through somatic embryogenic calli to exploit the somaclonal variation for its improvement. Various explants of variety Dante yellow were cultured on LS (Linsmaier and Skoog 1965) medium augmented with various concentrations of KT (Kinetin) and 2,4-D (2,4-dichlorophenoxyacetic acid) and their combinations for callus induction. Embryogenic calli were proliferated and regenerated by using different plant growth regulators. Regenerated plantlets were acclimatized and evaluated for agronomic characteristics and compared with mother plant in a replicated eld trial. Results revealed that young leaf explant cultured on LS medium containing 9.02 µM 2,4-D and 11.61 µM KT gave an ample amount of callus. Combination of 0.44 µM BAP (6-benzylaminopurine) and 5.37 µM NAA (1-naphthaleneacetic acid) yielded highest amount of callus proliferation (0.27g ± 0.03).Signi cant amount of shootlets (25 ± 0.8) from embryogenic callus was observed on the medium augmented with 0.45 µM 2, 4-D. During the eld experiment, Clone S84 showed considerable improvement in ower size as compare to mother plant and found to be a promising clone for commercialization.

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Section: Screening Of Somaclonesmentioning

confidence: 60%

Regeneration of chrysanthemum (Chrysanthemum morifolium) via somatic embryogenesis and screening of clones for agronomic traits

Ali

Raza

Shahzad

et al. 2023

Plant Cell Tiss Organ Cult

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“…Eventually, the mature plants reverted to typically shaped leaves. Changes in leaf shape from EMS exposure have been described in Abelmoschus esculentus (Gupta and Sood 2019), Chrysanthemum morifolium (Nasri et al 2021), and Cucumis sativus (Chen et al 2018).…”

Section: Resultsmentioning

confidence: 99%

Mutation Breeding of Salvia coccinea with Ethyl Methanesulfonate

Maynard

Ruter

2023

horts

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Salvia coccinea is a valuable flowering annual that attracts hummingbirds and bees to the garden, but few cultivars are commercially available. There is a limited range of petal colors and no leaf variegation. This research aimed to improve the ornamental value of S. coccinea by inducing mutations with ethyl methanesulfonate (EMS). The standard, red-flowered species was selected for treatment by exposing seeds to 0%, 0.4%, 0.8%, or 1.2% EMS for 8, 12, or 24 hours. The optimal treatment rate was determined to be 1.2% EMS for 8 hours, which generated desirable mutations near the median lethal dose (LD50). The M1 population had a 53% germination rate and was completely morphologically uniform. By the M2, mutations included differences in leaf shape and flower size in addition to albina, chlorina, virescens, and chimeral chlorophyll changes. A 1% mutation rate was achieved in this breeding program with seven unstable mutations and six stable mutations. The normalized difference vegetation index (NDVI) values were measured to determine differences in chlorophyll content between lethal albina mutations, chartreuse chlorina and virescens mutations, and typical leaf color. Future work will investigate the stability and heritability of chlorophyll variegation by hybridizing these selections with coral-flowered accessions of S. coccinea.

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“… Nasri et al. (2022) mutated four Chrysanthemum varieties (“ Homa ”, “ Fariba2 ”, “ Arina ”, and “ Delkash ”) with EMS (0-0.5%) as mutagens and leaf discs as explants to obtain new varieties.…”

Section: The Principles Methods and Process Of Ems Mutagenesismentioning

confidence: 99%

“…In particular, the EMS mutant population of sweet pepper variety 'Maoer' was established to study the genes regulating flower and plant structure (Siddiqe MI et al, 2020). Nasri et al (2022) mutated four Chrysanthemum varieties ("Homa", "Fariba2", "Arina", and "Delkash") with EMS (0-0.5%) as mutagens and leaf discs as explants to obtain new varieties. In addition, ISSR and IRAP molecular markers were used to detect the genetic polymorphism between the mutant and its parents.…”

Section: 372% Ems For 1 H and 2 H Determination Of Seed Oil Content A...mentioning

confidence: 99%

Current trends and insights on EMS mutagenesis application to studies on plant abiotic stress tolerance and development

Chen

Liu²,

Sun³

et al. 2023

Front. Plant Sci.

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Ethyl methanesulfonate (EMS)-induced mutagenesis is a powerful tool to generate genetic resource for identifying untapped genes and characterizing the function of genes to understand the molecular basis of important agronomic traits. This review focuses on application of contemporary EMS mutagenesis in the field of plant development and abiotic stress tolerance research, with particular focuses on reviewing the mutation types, mutagenesis site, mutagen concentration, mutagenesis duration, the identification and characterization of mutations responsible for altered stress tolerance responses. The application of EMS mutation breeding combined with genetic engineering in the future plant breeding and fundamental research was also discussed. The collective information in this review will provide good insight on how EMS mutagenesis is efficiently applied to improve abiotic stress tolerance of crops with the utilization of Next-generation sequencing (NGS) for mutation identification.

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In vitro mutagenesis of Chrysanthemum morifolium cultivars using ethylmethanesulphonate (EMS) and mutation assessment by ISSR and IRAP markers

Cited by 23 publications

References 64 publications

Regeneration of chrysanthemum (Chrysanthemum morifolium) via somatic embryogenesis and screening of clones for agronomic traits

Regeneration of chrysanthemum (Chrysanthemum morifolium) via somatic embryogenesis and screening of clones for agronomic traits

Mutation Breeding of Salvia coccinea with Ethyl Methanesulfonate

Current trends and insights on EMS mutagenesis application to studies on plant abiotic stress tolerance and development

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