In vitro mutagenicity, NMR metabolite characterization of azo and triphenylmethanes dyes by adherents bacteria and the role of the “cna” adhesion gene in activated sludge

Ayed, Lamia; Bakir, Karima; Mansour, Hédi Ben; Hammami, S.; Cheref, Abdelkrim; Bakhrouf, Amina

doi:10.1016/j.micpath.2016.12.016

Cited by 24 publications

(17 citation statements)

References 30 publications

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“…Azoreductases synthesized by azo dye decolorizing bacteria catalyze the azo bond under reducing conditions and have been documented in different species of Aeromonas, Enterococcus, Pseudomonas and Staphylococcus . A number of studies have shown that various microorganisms are able to degrade synthetic textile dyes in liquid medium and can be used for the treatment of textile effluent to prevent contamination of soil and water . The results imply that our isolated bacteria also carry an efficient enzymatic system for the cleavage of the azo bond present in reactive dyes.…”

Section: Discussionmentioning

confidence: 70%

Evaluation of bacteria isolated from textile wastewater and rhizosphere to simultaneously degrade azo dyes and promote plant growth

Shafqat

Khalid

Mahmood

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND: The release of untreated textile effluent to the environment and its subsequent application to cropland poses a serious threat to human health. Various bacterial species contained in the effluent can degrade azo dyes; however, no work has been done to elucidate the role of these bacteria for plant growth promotion (PGP). This study was designed to isolate and evaluate bacterial strains carrying dual traits for azo dye degradation and PGP. RESULTS: About 468 bacterial isolates were collected from textile effluent, sludge and dye-contaminated soil through enrichment in mineral salt medium (MSM) containing reactive black-5 dye (100 mg L -1 ). Seven isolates (I-15, S-10, 7.3, 11.4, AE-5, AE-7 and AE-8) with promising potential to treat the dye (100% color removal in 12-24 h) also exhibited significant production of indole acetic acid (9-21 g mL -1 ) and halo ring formation (diameter 6-11 mm), which is indicative of phosphate solubilization (P-solubilization). Inoculation of the isolates to maize (Zea mays) plants grown in water and MSM containing the dye resulted in decolorization of the dye while significantly improving plant biomass compared with the control plants. CONCLUSIONS: Our findings show that bacterial isolates possessing dual traits could be used simultaneously for textile effluent treatment and PGP. BOX polymerase chain reaction (BOX-PCR) and 16S rRNA gene amplificationThe BOX-PCR was performed in order to screen unique isolates before sequencing. Only those isolates with unique BOX-PCR fingerprints were sequenced by targeting a part of their 16S rRNA /jctb mustard (Brassica campestris L.) irrigated with distillery and tannery effluents. J Hazard Mater 162:1514-1521 (2009). 62 Garg V and Kaushik P, Influence of textile mill wastewater irrigation on the growth of sorghum cultivars. Appl Ecol Environ Res 6:1-12 (2008).

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Section: Discussionmentioning

confidence: 70%

Evaluation of bacteria isolated from textile wastewater and rhizosphere to simultaneously degrade azo dyes and promote plant growth

Shafqat

Khalid

Mahmood

et al. 2017

J of Chemical Tech & Biotech

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“…In addition, the degradation products were smaller compared to those reported in previous studies (Li et al, 2020;Zhang et al, 2017), which may suggest the degradation was more impactful using this method. Aniline blue has high phytotoxicity, high residue level, and other toxic and adverse effects (Lu et al, 2019), probably owing to the lipophilicity and binding to the cell membrane (Ayed et al, 2017). The phytotoxicity of aniline blue and its degraded metabolites was assessed in this study using Chinese cabbage seeds, and their corresponding germination percentage, root length, and seedling length were measured and used as phytotoxicity indices.…”

Section: Discussionmentioning

confidence: 99%

“…The biological method involves microbial degradation and biological enzymatic degradation (Zhang et al, 2017). The chemical and physical methods have disadvantages of high cost, low efficiency, and secondary contamination (Ayed et al, 2017;Holkar et al, 2014). Microbial degradation is reportedly unsuitable for aquatic environments, and the dye degradation potential of microorganisms in aquatic environments is low, probably because these microorganisms do not grow efficiently in aquatic environments (Li et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

New cost-effective mediator enhanced enzymatic degradation of aniline blue

Lu¹,

Luo²,

Dong³

et al. 2021

JEB

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Aim: In this study, the effects of various agricultural wastes as low-cost, readily available alternatives to chemical mediators for the enzymatic degradation of aniline blue were investigated. Methodology: Enzymes for degrading aniline blue were obtained through solid-state fermentation (SSF) of Pleurotus eryngii. Agricultural waste including grape skin/seed and orange peel, rich in organic acids, were used instead of chemical reagents as mediators. The degradation products were subjected to liquid chromatography-mass spectrometry (LC-MS) analysis. Results: The results show that the enzymes manganese peroxidase and versatile peroxidase were associated with aniline blue degradation. The organic acid-rich agricultural waste (grape seed/skin, orange peels) serving as substitutes for chemical reagents as mediators reduced the cost of the mediator by 99.8% at best, and the 2 hr degradation rate of aniline blue was 97.4% and 95.8%, respectively, increased by at least 4.3%. The LC-MS analysis revealed that aniline blue was degraded into smaller compounds with significantly lower toxicity. Interpretation: Overall, the enzyme–organic acid-rich agricultural waste mediator (grape seed/skin) system effectively degraded aniline blue. This cheap and efficient method for aniline blue degradation can serve as an environmentally friendly technology to treat waste with waste. Key words: Agricultural waste, Aniline blue, Enzymatic degradation, Organic acids, Pleurotus eryngii

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“…The RBO 3R and metabolic product extracted by ethyl acetate were dissolved separately in distilled water to obtain a solution of 1,000 ppm. The mutagenicity assay with Salmonella Typhimurium TA98 and TA100 was performed as described by Ayed et al [2]. The cytotoxicity test was carried out using human epithelial cells (Hep G2) and dedication of cell viability according to Ayed et al [23][24][25].…”

Section: Phytoxicity Mutagenicity Cytotoxicity and Biotoxicity Assmentioning

confidence: 99%

“…[1]. Dyes are toxic, carcinogenic, mutagenic, and teratogenic to humans and other life forms [2]. Synthetic dyes are divided into different classes on the basis of the chromophore group chemical structure [3].…”

Section: Introductionmentioning

confidence: 99%

Potential Use of Probiotic Consortium Isolated from Kefir for Textile Azo Dye Decolorization

Ayed¹,

Zmantar²,

Bayar³

et al. 2019

Journal of Microbiology and Biotechnology

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Azo dyes are recalcitrant pollutants, which are toxic, carcinogenic, mutagenic and teratogenic, that constitute a significant burden to the environment. The decolorization and the mineralization efficiency of Remazol Brillant Orange 3R (RBO 3R) was studied using a probiotic consortium (Lactobacillus acidophilus and Lactobacillus plantarum). Biodegradation of RBO 3R (750 ppm) was investigated under shaking condition in Mineral Salt Medium (MSM) solution at pH 11.5 and temperature 25 o C. The bio-decolorization process was further confirmed by FTIR and UV-Vis analysis. Under optimal conditions, the bacterial consortium was able to decolorize the dye completely (>99%) within 12 h. The color removal was 99.37% at 750 ppm. Muliplex PCR technique was used to detect the Lactobacillus genes. Using phytotoxicity, cytotoxicity, mutagenicity and biototoxicity endpoints, toxicological studies of RBO 3R before and after biodegradation were examined. A toxicity assay signaled that biodegradation led to detoxification of RBO 3R dye.

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In vitro mutagenicity, NMR metabolite characterization of azo and triphenylmethanes dyes by adherents bacteria and the role of the “cna” adhesion gene in activated sludge

Cited by 24 publications

References 30 publications

Evaluation of bacteria isolated from textile wastewater and rhizosphere to simultaneously degrade azo dyes and promote plant growth

Evaluation of bacteria isolated from textile wastewater and rhizosphere to simultaneously degrade azo dyes and promote plant growth

New cost-effective mediator enhanced enzymatic degradation of aniline blue

Potential Use of Probiotic Consortium Isolated from Kefir for Textile Azo Dye Decolorization

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